Influenza A virus (A/chicken/Gansu/2/99(H9N2))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H9N2 subtype

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0T1J0|A0T1J0_9INFA RNA-directed RNA polymerase catalytic subunit OS=Influenza A virus (A/chicken/Gansu/2/99(H9N2)) OX=412150 GN=PB1 PE=3 SV=1
MM1 pKa = 7.82EE2 pKa = 5.88DD3 pKa = 3.77FVRR6 pKa = 11.84QCFSPMIVEE15 pKa = 4.42LAEE18 pKa = 4.23KK19 pKa = 10.56AMKK22 pKa = 10.25EE23 pKa = 3.99YY24 pKa = 11.35GEE26 pKa = 4.4DD27 pKa = 3.65PKK29 pKa = 10.9IEE31 pKa = 4.27TNKK34 pKa = 9.76FAAICAHH41 pKa = 6.65LEE43 pKa = 4.03VCFMYY48 pKa = 10.77SDD50 pKa = 3.5FHH52 pKa = 8.77FIDD55 pKa = 3.6EE56 pKa = 4.51RR57 pKa = 11.84GEE59 pKa = 4.16SIIVEE64 pKa = 4.21FGDD67 pKa = 3.75PNVLLKK73 pKa = 10.76HH74 pKa = 5.97RR75 pKa = 11.84FEE77 pKa = 6.41IIEE80 pKa = 4.15GRR82 pKa = 11.84DD83 pKa = 2.82RR84 pKa = 11.84TMAWTVVNSICNTAGVEE101 pKa = 4.17KK102 pKa = 10.34PKK104 pKa = 10.62YY105 pKa = 10.59LPDD108 pKa = 4.59LYY110 pKa = 10.77DD111 pKa = 3.39YY112 pKa = 11.23KK113 pKa = 11.02EE114 pKa = 3.78NRR116 pKa = 11.84FIEE119 pKa = 4.17IGVTRR124 pKa = 11.84RR125 pKa = 11.84EE126 pKa = 3.67VHH128 pKa = 5.53TYY130 pKa = 10.32YY131 pKa = 10.84LEE133 pKa = 4.18KK134 pKa = 10.77ANKK137 pKa = 8.43IKK139 pKa = 10.59SEE141 pKa = 3.97RR142 pKa = 11.84THH144 pKa = 6.89IHH146 pKa = 5.65IFSFTGEE153 pKa = 3.88EE154 pKa = 4.15MATKK158 pKa = 10.42ADD160 pKa = 3.63YY161 pKa = 10.43TLDD164 pKa = 3.45EE165 pKa = 4.41EE166 pKa = 4.38SRR168 pKa = 11.84ARR170 pKa = 11.84IKK172 pKa = 10.03TRR174 pKa = 11.84LFTIRR179 pKa = 11.84QEE181 pKa = 4.06LASRR185 pKa = 11.84GLWDD189 pKa = 3.92SFRR192 pKa = 11.84QSEE195 pKa = 4.27RR196 pKa = 11.84GEE198 pKa = 3.89EE199 pKa = 4.16TIEE202 pKa = 3.68EE203 pKa = 4.17RR204 pKa = 11.84FEE206 pKa = 3.72ITGTMRR212 pKa = 11.84RR213 pKa = 11.84LADD216 pKa = 3.3QSLPPNFSSPEE227 pKa = 3.7NFRR230 pKa = 11.84AYY232 pKa = 11.01VDD234 pKa = 3.5GFEE237 pKa = 4.68PNGCIEE243 pKa = 4.47GKK245 pKa = 9.96LSQMSKK251 pKa = 10.05EE252 pKa = 3.75VTARR256 pKa = 11.84IEE258 pKa = 4.23PFLKK262 pKa = 8.2TTPRR266 pKa = 11.84PLKK269 pKa = 10.81LPDD272 pKa = 4.66GPPCSQRR279 pKa = 11.84SKK281 pKa = 10.91FLLMDD286 pKa = 4.4ALKK289 pKa = 10.88LSIEE293 pKa = 4.56DD294 pKa = 4.07PSHH297 pKa = 6.12EE298 pKa = 4.34GEE300 pKa = 5.39GIPLYY305 pKa = 10.72DD306 pKa = 5.54AIKK309 pKa = 10.45CMKK312 pKa = 9.82TFFGWKK318 pKa = 7.76EE319 pKa = 3.63PKK321 pKa = 9.75IIKK324 pKa = 9.11PHH326 pKa = 5.98EE327 pKa = 4.3KK328 pKa = 10.41GINPNYY334 pKa = 9.91LLAWKK339 pKa = 9.87QVLAEE344 pKa = 4.36LQDD347 pKa = 3.42IEE349 pKa = 4.52NDD351 pKa = 3.27EE352 pKa = 5.18KK353 pKa = 11.01IPKK356 pKa = 7.63TKK358 pKa = 10.62NMKK361 pKa = 8.74KK362 pKa = 8.8TSQLKK367 pKa = 8.56WALGEE372 pKa = 3.97NMAPEE377 pKa = 4.21KK378 pKa = 10.99VDD380 pKa = 5.68FEE382 pKa = 4.64DD383 pKa = 4.74CKK385 pKa = 11.37DD386 pKa = 3.67VGDD389 pKa = 4.39LKK391 pKa = 11.01QYY393 pKa = 11.25DD394 pKa = 3.81SDD396 pKa = 3.89EE397 pKa = 4.77PEE399 pKa = 4.58PKK401 pKa = 10.3SLASWIQSEE410 pKa = 4.32FNKK413 pKa = 10.5ACEE416 pKa = 4.4LTDD419 pKa = 3.89SSWVEE424 pKa = 3.47LDD426 pKa = 4.17EE427 pKa = 5.93IGEE430 pKa = 4.15DD431 pKa = 3.56VAPIEE436 pKa = 5.34HH437 pKa = 6.74IASMRR442 pKa = 11.84RR443 pKa = 11.84NYY445 pKa = 8.17FTAEE449 pKa = 3.92VSHH452 pKa = 6.6CRR454 pKa = 11.84ATEE457 pKa = 3.87YY458 pKa = 10.63IMKK461 pKa = 10.18GVYY464 pKa = 10.04INTALLNASCAAMDD478 pKa = 4.67DD479 pKa = 4.09FQLIPMISKK488 pKa = 10.11CRR490 pKa = 11.84TKK492 pKa = 10.42EE493 pKa = 3.77GRR495 pKa = 11.84RR496 pKa = 11.84KK497 pKa = 7.6TNLYY501 pKa = 10.52GFIIKK506 pKa = 9.94GRR508 pKa = 11.84SHH510 pKa = 7.02LRR512 pKa = 11.84NDD514 pKa = 3.25TDD516 pKa = 3.55VVNFVSMEE524 pKa = 4.12FSLTDD529 pKa = 3.59PRR531 pKa = 11.84LEE533 pKa = 3.7PHH535 pKa = 6.34KK536 pKa = 10.03WEE538 pKa = 4.54KK539 pKa = 10.88YY540 pKa = 9.17CVLEE544 pKa = 4.66VGDD547 pKa = 4.62MLLKK551 pKa = 10.16TSIGQVSRR559 pKa = 11.84PMFLYY564 pKa = 10.52VRR566 pKa = 11.84TNGTSKK572 pKa = 10.61IKK574 pKa = 9.96MKK576 pKa = 9.82WGMEE580 pKa = 3.71MRR582 pKa = 11.84RR583 pKa = 11.84CLLQSLQQIEE593 pKa = 4.49SMIEE597 pKa = 3.67AEE599 pKa = 4.44SSIKK603 pKa = 10.3EE604 pKa = 3.68KK605 pKa = 11.36DD606 pKa = 3.08MTKK609 pKa = 10.61EE610 pKa = 3.6FFEE613 pKa = 5.03NKK615 pKa = 9.97SEE617 pKa = 3.88MWPVGEE623 pKa = 4.18SPKK626 pKa = 10.72GVEE629 pKa = 4.03EE630 pKa = 4.32GSIGKK635 pKa = 8.56VCRR638 pKa = 11.84TLLAKK643 pKa = 10.3SVFNSLYY650 pKa = 10.64ASPQLEE656 pKa = 4.23GFSAEE661 pKa = 4.07SRR663 pKa = 11.84KK664 pKa = 10.13LLLIVQALRR673 pKa = 11.84DD674 pKa = 3.86NLEE677 pKa = 4.36PGTFDD682 pKa = 4.59IEE684 pKa = 4.3GLYY687 pKa = 10.04EE688 pKa = 4.75AIEE691 pKa = 4.31EE692 pKa = 4.51CLVNDD697 pKa = 3.92PWVLLNASWFNSFLTHH713 pKa = 6.92ALKK716 pKa = 10.78

Molecular weight: 82.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0T1J1|A0T1J1_9INFA Polymerase basic protein 2 OS=Influenza A virus (A/chicken/Gansu/2/99(H9N2)) OX=412150 GN=PB2 PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 6.09RR3 pKa = 11.84IKK5 pKa = 10.45EE6 pKa = 3.96LRR8 pKa = 11.84EE9 pKa = 3.83LMSQSRR15 pKa = 11.84TRR17 pKa = 11.84EE18 pKa = 3.86ILTKK22 pKa = 8.81TTVDD26 pKa = 2.86HH27 pKa = 5.76MAIIKK32 pKa = 10.06KK33 pKa = 7.84YY34 pKa = 7.91TSGRR38 pKa = 11.84QEE40 pKa = 4.09RR41 pKa = 11.84NPSLRR46 pKa = 11.84MKK48 pKa = 10.25WMMAMKK54 pKa = 10.45YY55 pKa = 10.07PITADD60 pKa = 3.19KK61 pKa = 10.67RR62 pKa = 11.84IMEE65 pKa = 4.94MIPEE69 pKa = 4.29RR70 pKa = 11.84NEE72 pKa = 3.3QGQILWSKK80 pKa = 9.58TNDD83 pKa = 3.26AGSNRR88 pKa = 11.84VMVSPLAVTWWNRR101 pKa = 11.84NGPTTSTVHH110 pKa = 5.17YY111 pKa = 8.49PKK113 pKa = 10.47VYY115 pKa = 8.32KK116 pKa = 10.23TYY118 pKa = 9.43FEE120 pKa = 4.01KK121 pKa = 11.11VEE123 pKa = 4.08RR124 pKa = 11.84LKK126 pKa = 11.04HH127 pKa = 4.72GTFGPVHH134 pKa = 6.7FRR136 pKa = 11.84NQVKK140 pKa = 9.39IRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84VDD146 pKa = 3.34VNPGHH151 pKa = 7.42ADD153 pKa = 3.81LSAKK157 pKa = 9.4EE158 pKa = 3.98AQDD161 pKa = 3.52VIMEE165 pKa = 4.23VVFPNEE171 pKa = 3.21VGARR175 pKa = 11.84ILTSEE180 pKa = 4.22SQLAITKK187 pKa = 8.39EE188 pKa = 4.21KK189 pKa = 10.81KK190 pKa = 10.3EE191 pKa = 3.92EE192 pKa = 3.95LQDD195 pKa = 3.83CKK197 pKa = 10.5IAPLMVAYY205 pKa = 9.47MLEE208 pKa = 4.18RR209 pKa = 11.84EE210 pKa = 4.67LVRR213 pKa = 11.84KK214 pKa = 7.75TRR216 pKa = 11.84FLPVSGGTSSVYY228 pKa = 10.33IEE230 pKa = 4.48VLHH233 pKa = 5.81LTQGICWEE241 pKa = 4.02QMYY244 pKa = 9.43TPGGEE249 pKa = 4.01VRR251 pKa = 11.84NDD253 pKa = 3.78DD254 pKa = 3.61VDD256 pKa = 3.41QSLIIAARR264 pKa = 11.84NIVRR268 pKa = 11.84RR269 pKa = 11.84ATVSADD275 pKa = 3.32PLLSLLEE282 pKa = 4.39MCHH285 pKa = 5.37STQIGGIRR293 pKa = 11.84MVDD296 pKa = 3.44ILRR299 pKa = 11.84QNPTEE304 pKa = 3.98EE305 pKa = 4.03QAVDD309 pKa = 2.96ICKK312 pKa = 10.16AALGLRR318 pKa = 11.84ISSSFSFGGFTFKK331 pKa = 10.07RR332 pKa = 11.84TSGSSVKK339 pKa = 10.45KK340 pKa = 10.2EE341 pKa = 3.91EE342 pKa = 4.07EE343 pKa = 4.18VLTGNLQTLRR353 pKa = 11.84IKK355 pKa = 10.1IHH357 pKa = 6.09EE358 pKa = 4.86GYY360 pKa = 10.82EE361 pKa = 3.92EE362 pKa = 3.95FTMVGRR368 pKa = 11.84RR369 pKa = 11.84ATAILRR375 pKa = 11.84KK376 pKa = 8.18ATKK379 pKa = 10.2RR380 pKa = 11.84LIQLIVSGRR389 pKa = 11.84DD390 pKa = 3.26EE391 pKa = 3.91QSIAEE396 pKa = 4.44AIIVAMVFSQEE407 pKa = 3.4EE408 pKa = 4.39CMIKK412 pKa = 10.34AGRR415 pKa = 11.84GDD417 pKa = 4.02LNFVNRR423 pKa = 11.84ANQRR427 pKa = 11.84LNPMHH432 pKa = 6.37QLLRR436 pKa = 11.84HH437 pKa = 5.04FQKK440 pKa = 10.58DD441 pKa = 3.4AKK443 pKa = 11.02VLFQNWGIEE452 pKa = 4.2PIDD455 pKa = 3.91NVMGMIGILPDD466 pKa = 3.56MTPSTEE472 pKa = 3.52MSLRR476 pKa = 11.84GVRR479 pKa = 11.84VSKK482 pKa = 10.41MGVDD486 pKa = 4.11EE487 pKa = 4.4YY488 pKa = 11.6SSTEE492 pKa = 3.83RR493 pKa = 11.84VTVSIDD499 pKa = 2.69RR500 pKa = 11.84FLRR503 pKa = 11.84VRR505 pKa = 11.84DD506 pKa = 3.39QLGNVLLSPEE516 pKa = 4.55EE517 pKa = 3.94VSEE520 pKa = 4.32TQGTEE525 pKa = 3.64KK526 pKa = 10.4LTITYY531 pKa = 9.18SSSMMWEE538 pKa = 3.7INGPEE543 pKa = 3.98SVLVNTYY550 pKa = 7.57QWIIRR555 pKa = 11.84NWEE558 pKa = 3.94TVKK561 pKa = 10.56IQWSQDD567 pKa = 2.67PTMLYY572 pKa = 10.81NKK574 pKa = 9.63MEE576 pKa = 4.24FEE578 pKa = 4.66PFQSLVPKK586 pKa = 10.32AARR589 pKa = 11.84SLYY592 pKa = 10.09SGFVRR597 pKa = 11.84TLFQQMRR604 pKa = 11.84DD605 pKa = 3.01ILGTFDD611 pKa = 3.19TVQIIKK617 pKa = 10.36LLPFAAAPPEE627 pKa = 3.92QSRR630 pKa = 11.84MQFSSLTVNVRR641 pKa = 11.84GSGMRR646 pKa = 11.84ILVRR650 pKa = 11.84GNSPVFNYY658 pKa = 10.9NKK660 pKa = 7.72VTKK663 pKa = 10.24RR664 pKa = 11.84LTVLGKK670 pKa = 10.13DD671 pKa = 3.2AGALTEE677 pKa = 5.33DD678 pKa = 4.67PDD680 pKa = 3.58EE681 pKa = 4.54GTAGVEE687 pKa = 4.29SAVLRR692 pKa = 11.84GFLILGKK699 pKa = 9.19EE700 pKa = 3.99DD701 pKa = 3.41RR702 pKa = 11.84RR703 pKa = 11.84YY704 pKa = 10.93GPALSISEE712 pKa = 4.47LSNLAKK718 pKa = 10.63GEE720 pKa = 4.09KK721 pKa = 10.32ANVLIGQGDD730 pKa = 3.9VVLVMKK736 pKa = 10.39RR737 pKa = 11.84KK738 pKa = 9.29RR739 pKa = 11.84DD740 pKa = 3.62SSILTDD746 pKa = 3.31SQTATKK752 pKa = 9.73RR753 pKa = 11.84IRR755 pKa = 11.84MAINN759 pKa = 3.15

Molecular weight: 85.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4225

217

759

528.1

59.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.846 ± 0.532

1.846 ± 0.399

4.521 ± 0.273

7.243 ± 0.731

3.645 ± 0.329

6.746 ± 0.565

1.68 ± 0.116

6.627 ± 0.359

5.728 ± 0.617

8.024 ± 0.603

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.621 ± 0.413

5.538 ± 0.656

3.953 ± 0.229

3.74 ± 0.313

6.817 ± 0.649

7.432 ± 0.42

6.722 ± 0.469

5.893 ± 0.44

1.538 ± 0.206

2.84 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski