Pseudomonas phage YMC11/06/C171_PPU_BP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Kantovirus; Pseudomonas virus C171

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127KNN6|A0A127KNN6_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC11/06/C171_PPU_BP OX=1777063 GN=C171_00010 PE=4 SV=1
MM1 pKa = 7.73LPQLQAAVHH10 pKa = 6.32KK11 pKa = 10.19GLSDD15 pKa = 3.74SLHH18 pKa = 6.16AAIDD22 pKa = 3.47TGNQEE27 pKa = 4.15EE28 pKa = 5.58VYY30 pKa = 10.6GAVDD34 pKa = 3.28TAEE37 pKa = 3.97EE38 pKa = 4.21AFALGQIDD46 pKa = 5.42AIQLGDD52 pKa = 3.97LLDD55 pKa = 4.86DD56 pKa = 3.88FRR58 pKa = 11.84IWEE61 pKa = 4.08EE62 pKa = 3.97SLLL65 pKa = 4.04

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127KNG8|A0A127KNG8_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC11/06/C171_PPU_BP OX=1777063 GN=C171_00180 PE=4 SV=1
MM1 pKa = 7.48AFEE4 pKa = 4.58SKK6 pKa = 11.26GNVRR10 pKa = 11.84YY11 pKa = 10.22NKK13 pKa = 9.4LAKK16 pKa = 10.13RR17 pKa = 11.84EE18 pKa = 4.16AKK20 pKa = 10.17VQEE23 pKa = 4.22LRR25 pKa = 11.84NEE27 pKa = 4.1GTPEE31 pKa = 3.8SRR33 pKa = 11.84KK34 pKa = 8.41QLKK37 pKa = 10.57AMLDD41 pKa = 3.51WRR43 pKa = 11.84PEE45 pKa = 3.77WEE47 pKa = 4.29NAGHH51 pKa = 6.54RR52 pKa = 11.84RR53 pKa = 11.84AVTPNGGMMHH63 pKa = 6.01NPKK66 pKa = 10.25GKK68 pKa = 9.33RR69 pKa = 11.84HH70 pKa = 5.34VKK72 pKa = 10.35GSSAKK77 pKa = 9.73CDD79 pKa = 3.94RR80 pKa = 11.84INRR83 pKa = 11.84GHH85 pKa = 6.92RR86 pKa = 11.84AKK88 pKa = 10.73LNQGKK93 pKa = 10.32GII95 pKa = 3.96

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13373

39

1264

272.9

29.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.351 ± 0.424

1.069 ± 0.16

6.438 ± 0.209

5.399 ± 0.314

3.343 ± 0.166

8.24 ± 0.316

1.78 ± 0.164

4.292 ± 0.184

4.031 ± 0.321

8.809 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.17

4.105 ± 0.242

4.247 ± 0.218

4.277 ± 0.298

5.96 ± 0.306

5.81 ± 0.274

6.364 ± 0.354

7.044 ± 0.29

1.496 ± 0.155

3.088 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski