Gaetbulibacter sp. 4G1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gaetbulibacter; unclassified Gaetbulibacter

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5GH64|A0A2G5GH64_9FLAO Uncharacterized protein OS=Gaetbulibacter sp. 4G1 OX=1891971 GN=BFR04_01600 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.55KK3 pKa = 10.42NILLLCIALLCVHH16 pKa = 6.46VLNAQCGPGEE26 pKa = 4.44DD27 pKa = 3.11TTAPVFGNAGDD38 pKa = 3.8GTMANPFRR46 pKa = 11.84NLLLSTVGGVPSGTYY61 pKa = 9.44YY62 pKa = 11.03FNFNGSTFQGALDD75 pKa = 4.11NDD77 pKa = 4.02TDD79 pKa = 3.91GGGWLMVLNYY89 pKa = 10.17VHH91 pKa = 7.36LAGDD95 pKa = 3.78NTDD98 pKa = 3.23LTIRR102 pKa = 11.84NTDD105 pKa = 3.47LPLLGSSTLGDD116 pKa = 3.8NEE118 pKa = 5.08AGTANWGHH126 pKa = 6.5MGNALAAAIDD136 pKa = 3.85FEE138 pKa = 4.79EE139 pKa = 3.83IRR141 pKa = 11.84FYY143 pKa = 11.63GEE145 pKa = 4.07TTGHH149 pKa = 4.66TRR151 pKa = 11.84IINFKK156 pKa = 8.74TPYY159 pKa = 9.94ISGLNYY165 pKa = 10.35LKK167 pKa = 10.16TGLGHH172 pKa = 7.11FGGINNPSNFTTLTGHH188 pKa = 6.32TANIPALAGNFFASHH203 pKa = 6.77GDD205 pKa = 3.34NALTEE210 pKa = 4.62FPFFSSGQNHH220 pKa = 5.55WGIRR224 pKa = 11.84GSGNRR229 pKa = 11.84WEE231 pKa = 4.8VDD233 pKa = 3.59DD234 pKa = 5.69APTAASPVNSQSTIHH249 pKa = 5.99RR250 pKa = 11.84VWVRR254 pKa = 11.84GDD256 pKa = 3.77LSPAGTPEE264 pKa = 4.18VSVTLDD270 pKa = 2.97NTGNVTVNPADD281 pKa = 3.65FGYY284 pKa = 8.86TVMDD288 pKa = 3.83NCSLEE293 pKa = 4.1ANINISLSQTDD304 pKa = 4.08FACIDD309 pKa = 3.32VGANIIQFTVTDD321 pKa = 3.87EE322 pKa = 4.37QNNSTVIDD330 pKa = 3.61VTVTVLEE337 pKa = 4.35SPPVITTPSGPFQRR351 pKa = 11.84ISLDD355 pKa = 3.65SNGEE359 pKa = 3.96LTLDD363 pKa = 3.92LATLNVTATDD373 pKa = 3.7DD374 pKa = 3.72CGIASTVISPATLDD388 pKa = 3.79CSDD391 pKa = 4.9AGFKK395 pKa = 10.54NATITVTDD403 pKa = 3.63VNGNASNSSMVFILIDD419 pKa = 3.32QVPPVIQCVPSYY431 pKa = 9.58TAEE434 pKa = 4.17LDD436 pKa = 3.59GTGSVTLDD444 pKa = 3.55PNSLLTSFTDD454 pKa = 3.28NCQTAGVSLDD464 pKa = 3.42KK465 pKa = 11.02TVFTCADD472 pKa = 3.08IGDD475 pKa = 4.08NLVTVTATDD484 pKa = 3.64GDD486 pKa = 4.67GNSTTCTTTVTITIPSCPGNLTLQAEE512 pKa = 4.35PNACGVTYY520 pKa = 10.4NYY522 pKa = 9.82PCASNVTAGPPSGTFLAVGTTTTFAYY548 pKa = 7.87DD549 pKa = 3.36TLDD552 pKa = 3.52NTGGTVTCSYY562 pKa = 11.24DD563 pKa = 3.27VTVVDD568 pKa = 4.3EE569 pKa = 4.75EE570 pKa = 5.19GPTFATQNITLDD582 pKa = 3.42VTMDD586 pKa = 3.46GTVSIVAEE594 pKa = 4.38DD595 pKa = 4.1LLGLDD600 pKa = 5.1LLASDD605 pKa = 4.18YY606 pKa = 11.15TVDD609 pKa = 3.46QTGTFNRR616 pKa = 11.84EE617 pKa = 4.27DD618 pKa = 3.19ISTTGTEE625 pKa = 3.53ISLFDD630 pKa = 4.21DD631 pKa = 3.9QVSSALPIGFEE642 pKa = 3.92FAFYY646 pKa = 10.46GALYY650 pKa = 10.06NDD652 pKa = 5.25FYY654 pKa = 10.94ISSNGFITFSGNSDD668 pKa = 3.83DD669 pKa = 4.73GCCTGQTLPDD679 pKa = 3.3VTEE682 pKa = 4.29PNNLIAFDD690 pKa = 3.81WDD692 pKa = 4.05DD693 pKa = 4.17YY694 pKa = 11.6DD695 pKa = 4.38PEE697 pKa = 5.63SGGTIRR703 pKa = 11.84YY704 pKa = 5.8QTIGTAPNRR713 pKa = 11.84ILIMDD718 pKa = 4.45FDD720 pKa = 4.69AVLHH724 pKa = 6.64IDD726 pKa = 3.77YY727 pKa = 10.94NDD729 pKa = 3.41NTDD732 pKa = 3.04ATTTQVKK739 pKa = 10.15LFEE742 pKa = 4.31GTNRR746 pKa = 11.84IEE748 pKa = 3.75IHH750 pKa = 5.91ATNIPDD756 pKa = 4.23LSNTKK761 pKa = 8.31TQGLEE766 pKa = 4.23NIDD769 pKa = 3.29GTAAIIVPGRR779 pKa = 11.84NAATWSASNDD789 pKa = 3.02VVAFVPVTGGVADD802 pKa = 3.64SCGVDD807 pKa = 3.2TFVASQTTFDD817 pKa = 4.5CSNLGANTVTLTATDD832 pKa = 3.54TNGNITEE839 pKa = 4.77KK840 pKa = 10.49IAIVTVTDD848 pKa = 4.22ANNFCPDD855 pKa = 3.11ILVSPKK861 pKa = 10.36VFLQGAALNPNVGEE875 pKa = 4.12EE876 pKa = 3.94TLMRR880 pKa = 11.84DD881 pKa = 3.98DD882 pKa = 5.64LRR884 pKa = 11.84IAGQIPTTSPYY895 pKa = 10.55TDD897 pKa = 4.25GLTCDD902 pKa = 3.55ASVFTVTGANAIVDD916 pKa = 4.23WVWLEE921 pKa = 3.69LRR923 pKa = 11.84DD924 pKa = 4.79PITSTTIVSSRR935 pKa = 11.84SALLQRR941 pKa = 11.84DD942 pKa = 3.61GDD944 pKa = 3.91IVDD947 pKa = 3.6IDD949 pKa = 4.06GVSTSVIFSEE959 pKa = 5.13VPGAYY964 pKa = 9.97YY965 pKa = 10.65LVVTHH970 pKa = 7.08RR971 pKa = 11.84NHH973 pKa = 6.83LGIRR977 pKa = 11.84SANTYY982 pKa = 9.88NLSSTTTTINLSTSSNSVAGGTNAIIDD1009 pKa = 3.99LGNGKK1014 pKa = 10.09FGLIGGDD1021 pKa = 3.66FDD1023 pKa = 6.99ANGQVQNTDD1032 pKa = 3.04LSGVRR1037 pKa = 11.84PVLGQSQYY1045 pKa = 11.52SAGDD1049 pKa = 3.48LDD1051 pKa = 4.62MNSQVQNTDD1060 pKa = 2.38INNLLNPNTGKK1071 pKa = 10.77GKK1073 pKa = 9.98QFAKK1077 pKa = 10.41QNLRR1081 pKa = 11.84AKK1083 pKa = 10.12LKK1085 pKa = 10.52KK1086 pKa = 10.36

Molecular weight: 114.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5GGQ7|A0A2G5GGQ7_9FLAO Uncharacterized protein OS=Gaetbulibacter sp. 4G1 OX=1891971 GN=BFR04_01035 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3344

0

3344

1180465

50

3150

353.0

39.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.985 ± 0.041

0.738 ± 0.014

5.673 ± 0.034

6.273 ± 0.04

5.357 ± 0.038

6.269 ± 0.046

1.757 ± 0.019

8.353 ± 0.041

8.259 ± 0.057

9.117 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.02

6.895 ± 0.042

3.227 ± 0.024

3.122 ± 0.022

3.173 ± 0.025

6.703 ± 0.034

5.804 ± 0.05

6.024 ± 0.028

1.091 ± 0.017

4.177 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski