Rubritalea profundi

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Rubritaleaceae; Rubritalea

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7U2U6|A0A2S7U2U6_9BACT Sec-independent protein translocase protein TatC OS=Rubritalea profundi OX=1658618 GN=tatC PE=3 SV=1
MM1 pKa = 6.84GHH3 pKa = 6.36NMGLSHH9 pKa = 7.61DD10 pKa = 4.13TTTSDD15 pKa = 3.21TIGYY19 pKa = 9.16YY20 pKa = 10.32GGHH23 pKa = 5.71GTGDD27 pKa = 3.9TSWGPIMGTGFNRR40 pKa = 11.84DD41 pKa = 2.8VTQWSKK47 pKa = 10.96GDD49 pKa = 3.56YY50 pKa = 8.21YY51 pKa = 11.63NSYY54 pKa = 10.98NEE56 pKa = 4.22STDD59 pKa = 3.52DD60 pKa = 3.71DD61 pKa = 4.38LAIISARR68 pKa = 11.84VPYY71 pKa = 10.44LADD74 pKa = 5.09DD75 pKa = 4.26YY76 pKa = 11.89GDD78 pKa = 5.57DD79 pKa = 4.5IATATPWFSEE89 pKa = 4.13TDD91 pKa = 3.59GSITQAGLIEE101 pKa = 4.47TTDD104 pKa = 3.75DD105 pKa = 3.82PDD107 pKa = 3.61VFEE110 pKa = 4.91FATGAGTVVFNANTFKK126 pKa = 11.03SDD128 pKa = 3.19AANSHH133 pKa = 5.59TNGGNLDD140 pKa = 3.59INLEE144 pKa = 4.17LRR146 pKa = 11.84DD147 pKa = 3.78SSGTLIALDD156 pKa = 3.93NPALGMNASISQSVAAGNYY175 pKa = 8.31FLFVIPSGAGSPLASSPTGYY195 pKa = 9.18TSYY198 pKa = 11.52GSLGQYY204 pKa = 10.2SITGTIVPTDD214 pKa = 3.74QIALTSPNGGEE225 pKa = 3.28EE226 pKa = 4.17WMRR229 pKa = 11.84STSQPVTWLSGMGGHH244 pKa = 6.67VKK246 pKa = 10.08IEE248 pKa = 4.03LFKK251 pKa = 11.3GGSLDD256 pKa = 3.8SSLAANTPNDD266 pKa = 3.61GLFNWVIPSDD276 pKa = 3.73QAEE279 pKa = 4.27GTDD282 pKa = 3.7YY283 pKa = 10.82KK284 pKa = 10.94IKK286 pKa = 9.37ITSIDD291 pKa = 3.6QPSKK295 pKa = 10.77LDD297 pKa = 3.45KK298 pKa = 11.01SSLNFSVLLEE308 pKa = 4.52SNDD311 pKa = 3.25ILIANMDD318 pKa = 4.39ADD320 pKa = 4.58PGFSTTGLFEE330 pKa = 4.24FGIPSGDD337 pKa = 3.16NDD339 pKa = 4.16AANTNTGTNIYY350 pKa = 9.07DD351 pKa = 3.54TDD353 pKa = 4.39LDD355 pKa = 4.29STCFTASTLTTYY367 pKa = 11.42ALDD370 pKa = 4.13CSNHH374 pKa = 6.17SNVSLEE380 pKa = 4.36FWAHH384 pKa = 5.72TYY386 pKa = 10.37LYY388 pKa = 9.59PDD390 pKa = 3.34YY391 pKa = 9.66TAVFEE396 pKa = 4.64VSNDD400 pKa = 3.59NINWTPLLSQAGMSTRR416 pKa = 11.84SWTKK420 pKa = 8.32YY421 pKa = 9.57SYY423 pKa = 10.82NISSVADD430 pKa = 3.98GQPTVYY436 pKa = 10.31VRR438 pKa = 11.84WSMLGSGTQRR448 pKa = 11.84PGGGLAIDD456 pKa = 4.47DD457 pKa = 4.22AQVVGDD463 pKa = 5.63FVPADD468 pKa = 4.02DD469 pKa = 5.66VSLTSPNGGEE479 pKa = 3.55NWIPNIQNQITWNSSMGGNVKK500 pKa = 9.95IEE502 pKa = 3.95LLEE505 pKa = 4.63GGSVNAVIALSTPNDD520 pKa = 3.59GSYY523 pKa = 11.09LWTAPTAQMLGGDD536 pKa = 3.88YY537 pKa = 10.37KK538 pKa = 11.41VRR540 pKa = 11.84IQSIEE545 pKa = 3.45NTFPYY550 pKa = 10.02RR551 pKa = 11.84RR552 pKa = 11.84EE553 pKa = 3.79

Molecular weight: 59.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7U3Q9|A0A2S7U3Q9_9BACT Protein RecA OS=Rubritalea profundi OX=1658618 GN=recA PE=3 SV=1
RR1 pKa = 7.43PCGIGYY7 pKa = 8.04LAPLGSRR14 pKa = 11.84RR15 pKa = 11.84ALSFWQITPAFVSFEE30 pKa = 4.18SLSGIKK36 pKa = 9.91IFFHH40 pKa = 6.33CALRR44 pKa = 11.84GASQRR49 pKa = 11.84INSTKK54 pKa = 9.76PNPYY58 pKa = 9.61SHH60 pKa = 6.42QKK62 pKa = 9.89NLRR65 pKa = 11.84LCGSARR71 pKa = 11.84GFEE74 pKa = 4.53FISRR78 pKa = 11.84AEE80 pKa = 4.08PHH82 pKa = 6.64SKK84 pKa = 8.55TT85 pKa = 3.73

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3389

0

3389

1083921

15

2907

319.8

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.847 ± 0.042

1.086 ± 0.014

5.618 ± 0.032

6.291 ± 0.037

4.03 ± 0.025

7.313 ± 0.041

2.299 ± 0.021

6.032 ± 0.031

6.1 ± 0.045

9.899 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.02

3.805 ± 0.029

4.443 ± 0.022

3.634 ± 0.028

4.987 ± 0.032

6.859 ± 0.04

5.557 ± 0.039

6.513 ± 0.038

1.415 ± 0.019

2.876 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski