Paraburkholderia lycopersici

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6THB8|A0A1G6THB8_9BURK Uncharacterized protein OS=Paraburkholderia lycopersici OX=416944 GN=SAMN05421548_11725 PE=4 SV=1
MM1 pKa = 6.95STVSSTASTIASTVASTTSASNAALQAAAQSIISGSTNSTMDD43 pKa = 3.23VNSLVTALVNSKK55 pKa = 10.08IAGQAATIAAEE66 pKa = 3.97QSADD70 pKa = 3.39NTEE73 pKa = 3.54ISAYY77 pKa = 8.13GTLSAALSALQAAVLPLSNGSTISSFSTSLDD108 pKa = 3.24GTGITATAGAGAVASSYY125 pKa = 11.18SLNVQQIAQAQVLTSAAYY143 pKa = 8.0TGPLVSAGTATMTLTVNGASTTISLNNSNSTVAGIASAINSASNNPGVTATVVNGADD200 pKa = 3.7GAHH203 pKa = 6.3LVLHH207 pKa = 5.66STSTGSSNGIGISIKK222 pKa = 10.68DD223 pKa = 3.87SNTSDD228 pKa = 3.39ALNGLSVTTAAGTAITSSSADD249 pKa = 3.41SAALLANQSTIVSGTDD265 pKa = 3.12PTTGLAGWKK274 pKa = 9.78QSVAAQDD281 pKa = 3.66AYY283 pKa = 10.77LTVDD287 pKa = 3.3NTGVTSSSNTVTTGVSGVTLNLTSAAIGTTQTLTVSQDD325 pKa = 2.81TSTQSAQINNFVSLYY340 pKa = 7.92NTLVTTMGTLTSYY353 pKa = 11.37SSTSTSQGVLLGDD366 pKa = 3.34PTLNVIRR373 pKa = 11.84NQLASIVSSAVGTGTNAHH391 pKa = 5.77TLGAIGITLQSDD403 pKa = 4.26GSLSIDD409 pKa = 3.88SDD411 pKa = 4.01TLNNALANDD420 pKa = 3.64QSTLSTLFNSTNGIAAQLNKK440 pKa = 10.51NVTNYY445 pKa = 9.19TSSTGIIQNRR455 pKa = 11.84TDD457 pKa = 3.97ALNADD462 pKa = 4.21LTSLSNQQTTLSTWQDD478 pKa = 3.48QLTSMYY484 pKa = 10.15NAQFTALNTLMATMNNNTQYY504 pKa = 10.12LTQLFGGTNSAGALATAKK522 pKa = 10.45SS523 pKa = 3.6

Molecular weight: 51.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7A040|A0A1G7A040_9BURK GMP synthase [glutamine-hydrolyzing] OS=Paraburkholderia lycopersici OX=416944 GN=guaA PE=3 SV=1
MM1 pKa = 7.12LTSSLLLLGVAAVLGQVAWTRR22 pKa = 11.84ARR24 pKa = 11.84ASLQPVRR31 pKa = 11.84VRR33 pKa = 11.84ATQPRR38 pKa = 11.84RR39 pKa = 11.84FRR41 pKa = 4.46

Molecular weight: 4.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6389

0

6389

2038431

26

3320

319.1

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.305 ± 0.046

0.971 ± 0.011

5.33 ± 0.023

5.275 ± 0.03

3.662 ± 0.02

8.267 ± 0.037

2.347 ± 0.015

4.494 ± 0.023

2.902 ± 0.021

10.204 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.014

2.742 ± 0.02

5.127 ± 0.028

3.551 ± 0.02

7.291 ± 0.032

5.472 ± 0.026

5.245 ± 0.025

7.705 ± 0.024

1.393 ± 0.014

2.408 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski