Garlic virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus; Acarallexivirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|O09499|O09499_9VIRU Helicase OS=Garlic virus X OX=150284 PE=4 SV=1
MM1 pKa = 6.78TTVQKK6 pKa = 11.0LLDD9 pKa = 4.13QISDD13 pKa = 3.73PNARR17 pKa = 11.84AAYY20 pKa = 10.16SQDD23 pKa = 3.41CLSAAKK29 pKa = 10.15NAATKK34 pKa = 11.03AMTIAPYY41 pKa = 10.82AVTQTEE47 pKa = 4.29ALTLEE52 pKa = 4.81RR53 pKa = 11.84IGITTSPFATTSNTHH68 pKa = 6.16AADD71 pKa = 4.2KK72 pKa = 10.59IIEE75 pKa = 4.22NDD77 pKa = 3.33CLEE80 pKa = 4.37IIGRR84 pKa = 11.84YY85 pKa = 8.47LPKK88 pKa = 10.68EE89 pKa = 4.03PVTLIQLKK97 pKa = 9.75RR98 pKa = 11.84AKK100 pKa = 10.29LHH102 pKa = 5.43LLKK105 pKa = 10.58RR106 pKa = 11.84GPNTDD111 pKa = 2.38IFQNYY116 pKa = 7.15CHH118 pKa = 6.58EE119 pKa = 4.73PKK121 pKa = 10.52DD122 pKa = 3.63VLRR125 pKa = 11.84FGLTNPHH132 pKa = 5.72VCPVVNTSCAVISDD146 pKa = 4.03TLHH149 pKa = 6.31FMSSQQVWTLFKK161 pKa = 10.81RR162 pKa = 11.84NPKK165 pKa = 9.49LQRR168 pKa = 11.84LYY170 pKa = 9.82GTLVLPVEE178 pKa = 5.0ALNKK182 pKa = 10.18LPSLFPEE189 pKa = 4.6IYY191 pKa = 10.29KK192 pKa = 10.57LEE194 pKa = 4.36YY195 pKa = 9.78YY196 pKa = 10.35DD197 pKa = 3.79NHH199 pKa = 6.38FAYY202 pKa = 9.97MPGGHH207 pKa = 6.74GGGAYY212 pKa = 8.55IHH214 pKa = 7.06SYY216 pKa = 8.85GTLKK220 pKa = 10.24WLSTAQIGTEE230 pKa = 4.13SNHH233 pKa = 6.12LSIEE237 pKa = 4.23KK238 pKa = 10.15IEE240 pKa = 4.33TKK242 pKa = 10.12AAHH245 pKa = 6.96HH246 pKa = 6.53IMLIQRR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84PEE256 pKa = 4.56LPWPLPPLWVFHH268 pKa = 6.55ASDD271 pKa = 4.09YY272 pKa = 10.96VRR274 pKa = 11.84LPEE277 pKa = 3.9IFYY280 pKa = 10.1PSEE283 pKa = 4.18ANVQRR288 pKa = 11.84AYY290 pKa = 9.87PHH292 pKa = 6.03VFIKK296 pKa = 10.56RR297 pKa = 11.84LQLYY301 pKa = 8.31CFSVKK306 pKa = 10.2AVSLRR311 pKa = 11.84DD312 pKa = 3.18IFAKK316 pKa = 9.72IRR318 pKa = 11.84QVIPTEE324 pKa = 4.06DD325 pKa = 3.34LSRR328 pKa = 11.84FSTADD333 pKa = 4.48LIRR336 pKa = 11.84LANYY340 pKa = 10.44LLFITGMQQTSDD352 pKa = 3.74YY353 pKa = 10.41QSPLMEE359 pKa = 4.71SLLGKK364 pKa = 10.15LCLSIRR370 pKa = 11.84ARR372 pKa = 11.84VRR374 pKa = 11.84EE375 pKa = 4.06FFQNLLGKK383 pKa = 9.96SSYY386 pKa = 10.95AALLTVTDD394 pKa = 3.49VRR396 pKa = 11.84PVYY399 pKa = 9.33FTTKK403 pKa = 9.88PIRR406 pKa = 11.84RR407 pKa = 11.84PICGQPWFDD416 pKa = 3.55EE417 pKa = 4.76AEE419 pKa = 4.38VEE421 pKa = 4.7SKK423 pKa = 10.48PATPTITGYY432 pKa = 10.09PNPSPDD438 pKa = 5.05DD439 pKa = 4.15LDD441 pKa = 4.19LLAEE445 pKa = 4.52FEE447 pKa = 4.23QHH449 pKa = 5.0EE450 pKa = 4.19RR451 pKa = 11.84AMMEE455 pKa = 4.36EE456 pKa = 3.99NCFTSKK462 pKa = 9.74TSATANRR469 pKa = 11.84AQNPQQNRR477 pKa = 11.84EE478 pKa = 3.74RR479 pKa = 11.84VTPEE483 pKa = 3.61KK484 pKa = 10.3LAHH487 pKa = 6.48SSDD490 pKa = 3.7EE491 pKa = 4.04SHH493 pKa = 6.06TQITSSQGVNDD504 pKa = 3.99HH505 pKa = 6.29TWPTEE510 pKa = 3.6QSQPTNTCKK519 pKa = 10.73EE520 pKa = 3.9ITDD523 pKa = 4.77DD524 pKa = 3.68EE525 pKa = 4.96NMMDD529 pKa = 3.73GHH531 pKa = 5.5VTDD534 pKa = 4.25WTDD537 pKa = 2.9SDD539 pKa = 4.64SDD541 pKa = 4.03HH542 pKa = 7.63SDD544 pKa = 3.24EE545 pKa = 6.35DD546 pKa = 4.39IEE548 pKa = 4.55TQPEE552 pKa = 4.32TNLSQSIHH560 pKa = 7.54DD561 pKa = 4.78DD562 pKa = 3.86LLPDD566 pKa = 4.2EE567 pKa = 5.66SDD569 pKa = 3.96LPDD572 pKa = 4.33LEE574 pKa = 4.9SVADD578 pKa = 4.08EE579 pKa = 4.65QSDD582 pKa = 4.08AEE584 pKa = 4.62DD585 pKa = 4.39NGDD588 pKa = 3.75STIDD592 pKa = 3.65QANLCNTTCDD602 pKa = 3.6AEE604 pKa = 4.42TDD606 pKa = 3.64KK607 pKa = 11.4SAQTPAEE614 pKa = 4.27AKK616 pKa = 10.21SSPKK620 pKa = 9.88SASRR624 pKa = 11.84VDD626 pKa = 4.0LTPLDD631 pKa = 3.93SDD633 pKa = 3.79TVEE636 pKa = 3.8ILRR639 pKa = 11.84LHH641 pKa = 6.78GFTNYY646 pKa = 9.77KK647 pKa = 8.53PQHH650 pKa = 6.67DD651 pKa = 4.76GDD653 pKa = 4.02FQIAPVFYY661 pKa = 10.68NHH663 pKa = 6.09QACNLPLYY671 pKa = 10.76DD672 pKa = 5.57GITNSAQSKK681 pKa = 8.72FIEE684 pKa = 4.25MAHH687 pKa = 5.57ALKK690 pKa = 10.46RR691 pKa = 11.84NCYY694 pKa = 10.31NYY696 pKa = 9.74ILEE699 pKa = 4.42PRR701 pKa = 11.84RR702 pKa = 11.84ATAFMSDD709 pKa = 3.27VKK711 pKa = 11.38NNLTGLVLPKK721 pKa = 10.11LDD723 pKa = 4.63RR724 pKa = 11.84DD725 pKa = 4.2LLTSWVALSEE735 pKa = 4.15NACRR739 pKa = 11.84KK740 pKa = 9.44VGLIVIHH747 pKa = 6.15GAGGAGKK754 pKa = 10.05SRR756 pKa = 11.84ALQEE760 pKa = 3.94ILRR763 pKa = 11.84SNSIDD768 pKa = 3.48TNQVNIIVPTINLAVDD784 pKa = 3.84WKK786 pKa = 10.95KK787 pKa = 10.79KK788 pKa = 10.06LPKK791 pKa = 9.34IDD793 pKa = 3.65PRR795 pKa = 11.84RR796 pKa = 11.84VMTFEE801 pKa = 4.09KK802 pKa = 10.45ACEE805 pKa = 3.99RR806 pKa = 11.84EE807 pKa = 4.42GKK809 pKa = 10.11SVVIMDD815 pKa = 5.34DD816 pKa = 3.58YY817 pKa = 11.94GKK819 pKa = 10.9LPAGYY824 pKa = 8.89IDD826 pKa = 5.1AYY828 pKa = 10.95LAIKK832 pKa = 10.04FNVEE836 pKa = 3.71IVILTGDD843 pKa = 3.22QRR845 pKa = 11.84QSVFHH850 pKa = 7.11NDD852 pKa = 3.21RR853 pKa = 11.84KK854 pKa = 10.63DD855 pKa = 3.44SQIALLTSNTAHH867 pKa = 7.2FKK869 pKa = 10.47RR870 pKa = 11.84YY871 pKa = 9.29CDD873 pKa = 3.61YY874 pKa = 11.17YY875 pKa = 11.6LNATHH880 pKa = 6.81RR881 pKa = 11.84QPRR884 pKa = 11.84RR885 pKa = 11.84LANPIRR891 pKa = 11.84VHH893 pKa = 6.64AEE895 pKa = 3.83RR896 pKa = 11.84EE897 pKa = 3.84VGGAIKK903 pKa = 10.33HH904 pKa = 5.22STLIPTHH911 pKa = 6.63AMTLVPAFRR920 pKa = 11.84SQSLLTDD927 pKa = 4.09LGRR930 pKa = 11.84QAMTYY935 pKa = 10.18AGCQGLTLPHH945 pKa = 6.01LTIVLDD951 pKa = 4.36KK952 pKa = 10.04DD953 pKa = 3.68TPLCSDD959 pKa = 3.5EE960 pKa = 4.38VLYY963 pKa = 9.62TALSRR968 pKa = 11.84ASEE971 pKa = 4.34TITFVNTYY979 pKa = 10.81SNNKK983 pKa = 9.72DD984 pKa = 3.54FLDD987 pKa = 4.27KK988 pKa = 11.06LDD990 pKa = 3.66STPYY994 pKa = 10.72LKK996 pKa = 9.81TLISGVRR1003 pKa = 11.84EE1004 pKa = 3.99DD1005 pKa = 4.62EE1006 pKa = 4.17QVGWEE1011 pKa = 4.16NPPPEE1016 pKa = 4.8PEE1018 pKa = 4.22VRR1020 pKa = 11.84DD1021 pKa = 3.97APTKK1025 pKa = 7.99THH1027 pKa = 6.83IPVANNNVQLEE1038 pKa = 4.49SKK1040 pKa = 10.32IEE1042 pKa = 3.98TMEE1045 pKa = 4.78DD1046 pKa = 3.09KK1047 pKa = 10.11DD1048 pKa = 4.63TRR1050 pKa = 11.84EE1051 pKa = 3.87LWSGEE1056 pKa = 3.84EE1057 pKa = 4.18KK1058 pKa = 10.4TNLMQTQDD1066 pKa = 3.52PVIQLFPHH1074 pKa = 5.38QQAKK1078 pKa = 10.89DD1079 pKa = 3.24EE1080 pKa = 4.47ALFKK1084 pKa = 10.13ITINEE1089 pKa = 4.29RR1090 pKa = 11.84IKK1092 pKa = 10.87LASPEE1097 pKa = 4.39TNCKK1101 pKa = 9.64ALKK1104 pKa = 8.4EE1105 pKa = 4.16TLNTGNLLFEE1115 pKa = 5.23AYY1117 pKa = 10.21ADD1119 pKa = 3.65FMNVPRR1125 pKa = 11.84EE1126 pKa = 3.97TQPFDD1131 pKa = 3.22KK1132 pKa = 10.56RR1133 pKa = 11.84LWAHH1137 pKa = 6.37CRR1139 pKa = 11.84QLALRR1144 pKa = 11.84TYY1146 pKa = 10.54LSKK1149 pKa = 9.75PTTNLQQGAMQDD1161 pKa = 3.23PDD1163 pKa = 3.78FPEE1166 pKa = 3.79NAIALFNKK1174 pKa = 8.34SQWVKK1179 pKa = 10.35KK1180 pKa = 10.25LEE1182 pKa = 4.06KK1183 pKa = 10.47VGCKK1187 pKa = 9.95FKK1189 pKa = 11.02AGQTISAFKK1198 pKa = 10.55QSTVLLTTTMALYY1211 pKa = 10.12LRR1213 pKa = 11.84KK1214 pKa = 9.53KK1215 pKa = 10.33RR1216 pKa = 11.84EE1217 pKa = 3.63SHH1219 pKa = 5.86QPDD1222 pKa = 3.56NVFVMCEE1229 pKa = 3.73KK1230 pKa = 10.34TPEE1233 pKa = 4.02QFNSFVLTRR1242 pKa = 11.84WDD1244 pKa = 3.69FTRR1247 pKa = 11.84PNYY1250 pKa = 9.75TSDD1253 pKa = 3.13YY1254 pKa = 8.03TQYY1257 pKa = 11.34DD1258 pKa = 3.52QSQDD1262 pKa = 3.34SAFLNFEE1269 pKa = 4.01LRR1271 pKa = 11.84KK1272 pKa = 9.85AKK1274 pKa = 10.41HH1275 pKa = 6.16LGLPDD1280 pKa = 3.69EE1281 pKa = 4.85VVDD1284 pKa = 5.53FYY1286 pKa = 12.15AFIKK1290 pKa = 8.86THH1292 pKa = 6.42AKK1294 pKa = 9.19TFLGNLAVMRR1304 pKa = 11.84LSGEE1308 pKa = 4.14GPTFDD1313 pKa = 6.1ANTEE1317 pKa = 4.05CNIAYY1322 pKa = 8.34DD1323 pKa = 3.78ALRR1326 pKa = 11.84FQLDD1330 pKa = 3.55STVNASYY1337 pKa = 11.04AGDD1340 pKa = 3.71DD1341 pKa = 3.79LVRR1344 pKa = 11.84DD1345 pKa = 4.02RR1346 pKa = 11.84ARR1348 pKa = 11.84SEE1350 pKa = 4.04RR1351 pKa = 11.84PGWKK1355 pKa = 9.3YY1356 pKa = 11.13AEE1358 pKa = 4.31PLFSLKK1364 pKa = 10.51AKK1366 pKa = 10.13PLVTSKK1372 pKa = 10.96PDD1374 pKa = 3.17FCGWRR1379 pKa = 11.84LTKK1382 pKa = 10.71YY1383 pKa = 10.46GIVKK1387 pKa = 10.52SPVQLYY1393 pKa = 10.28QSLQLALRR1401 pKa = 11.84LGKK1404 pKa = 10.19VEE1406 pKa = 4.03EE1407 pKa = 4.42VKK1409 pKa = 10.49RR1410 pKa = 11.84SYY1412 pKa = 11.23AIDD1415 pKa = 3.38YY1416 pKa = 9.93LFAYY1420 pKa = 10.43RR1421 pKa = 11.84LGDD1424 pKa = 3.38QLYY1427 pKa = 10.56DD1428 pKa = 3.39IFDD1431 pKa = 3.81EE1432 pKa = 5.39DD1433 pKa = 4.24EE1434 pKa = 4.2LAKK1437 pKa = 10.5HH1438 pKa = 5.31QLVTRR1443 pKa = 11.84TLIKK1447 pKa = 10.51KK1448 pKa = 9.59GMQPPSSGEE1457 pKa = 3.8HH1458 pKa = 6.19LPTFHH1463 pKa = 6.1VTSDD1467 pKa = 3.01RR1468 pKa = 11.84LIRR1471 pKa = 11.84DD1472 pKa = 3.8PKK1474 pKa = 10.2AIQVLSYY1481 pKa = 10.1EE1482 pKa = 4.44SDD1484 pKa = 3.59RR1485 pKa = 11.84IMVPTDD1491 pKa = 3.2VIEE1494 pKa = 4.34DD1495 pKa = 3.91HH1496 pKa = 6.35FTLASRR1502 pKa = 11.84TDD1504 pKa = 3.83RR1505 pKa = 11.84DD1506 pKa = 3.96DD1507 pKa = 3.14MTAEE1511 pKa = 4.1SNRR1514 pKa = 11.84RR1515 pKa = 11.84ASGLCTPINPGDD1527 pKa = 3.72STGALLTDD1535 pKa = 4.26MFPSIATLL1543 pKa = 3.41

Molecular weight:
174.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|O09501|O09501_9VIRU 32 kDa protein OS=Garlic virus X OX=150284 PE=4 SV=1
MM1 pKa = 7.54SFTPPPDD8 pKa = 3.28HH9 pKa = 6.5TKK11 pKa = 10.94SFLALATGAAAGLLIYY27 pKa = 7.97TLRR30 pKa = 11.84SNQLAPVGDD39 pKa = 3.91NTHH42 pKa = 5.94QLPHH46 pKa = 6.52GGRR49 pKa = 11.84YY50 pKa = 8.86RR51 pKa = 11.84DD52 pKa = 3.45GNKK55 pKa = 9.04FVSYY59 pKa = 10.64NGPNTGASSHH69 pKa = 6.08SIWWPAITAITLTIVIHH86 pKa = 6.01FLSSTRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84VCVRR98 pKa = 11.84CSEE101 pKa = 4.3STLPHH106 pKa = 6.36

Molecular weight:
11.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2541

106

1543

423.5

47.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.477 ± 0.497

1.889 ± 0.334

5.864 ± 0.952

5.549 ± 0.557

4.211 ± 0.475

4.565 ± 0.735

3.109 ± 0.496

4.998 ± 0.266

5.195 ± 0.521

10.114 ± 0.687

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.85 ± 0.345

4.88 ± 0.389

6.061 ± 0.401

4.526 ± 0.439

5.549 ± 0.486

7.399 ± 0.651

8.107 ± 0.516

4.801 ± 0.372

0.826 ± 0.245

3.03 ± 0.299

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski