Pleurotus ostreatus virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Betapartitivirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5GHF1|Q5GHF1_9VIRU RNA-dependent RNA polymerase OS=Pleurotus ostreatus virus 1 OX=674983 PE=4 SV=1
MM1 pKa = 7.41SSSAARR7 pKa = 11.84IQTASRR13 pKa = 11.84VAPAEE18 pKa = 3.71IRR20 pKa = 11.84TPPALAAIAKK30 pKa = 9.48KK31 pKa = 9.42FAPVILQDD39 pKa = 3.32NDD41 pKa = 3.63VPVPPSNADD50 pKa = 2.98YY51 pKa = 10.32FGRR54 pKa = 11.84MNLPTNIEE62 pKa = 4.12LDD64 pKa = 3.19SGNYY68 pKa = 9.79HH69 pKa = 6.72NIDD72 pKa = 3.33VQFDD76 pKa = 3.36TRR78 pKa = 11.84FILNYY83 pKa = 10.1IMYY86 pKa = 9.61HH87 pKa = 5.24VMNLAPEE94 pKa = 4.61LNFKK98 pKa = 9.16GHH100 pKa = 7.38PYY102 pKa = 10.3FSPLSYY108 pKa = 9.75IGYY111 pKa = 9.84CMHH114 pKa = 7.32LFYY117 pKa = 11.22ALLLACDD124 pKa = 3.74CTFRR128 pKa = 11.84SDD130 pKa = 3.43KK131 pKa = 10.66SYY133 pKa = 10.31HH134 pKa = 6.47ASRR137 pKa = 11.84FMTDD141 pKa = 2.99QEE143 pKa = 4.41RR144 pKa = 11.84KK145 pKa = 9.99DD146 pKa = 3.61LYY148 pKa = 10.86EE149 pKa = 4.05VLLNCQMPTFLSDD162 pKa = 4.55LFLEE166 pKa = 4.8LAPVYY171 pKa = 10.36DD172 pKa = 3.83PRR174 pKa = 11.84RR175 pKa = 11.84NNLLFVPSLAGHH187 pKa = 5.71SFEE190 pKa = 5.06HH191 pKa = 7.56DD192 pKa = 3.36YY193 pKa = 11.63GRR195 pKa = 11.84TLLPSMFYY203 pKa = 10.33AAHH206 pKa = 6.6HH207 pKa = 5.98MLASTRR213 pKa = 11.84TNKK216 pKa = 10.37DD217 pKa = 3.26PNDD220 pKa = 3.97VIDD223 pKa = 4.66DD224 pKa = 3.93CMALHH229 pKa = 7.04VITAGTTNFTISNYY243 pKa = 10.28LGTWYY248 pKa = 8.76ATGHH252 pKa = 6.29HH253 pKa = 7.03DD254 pKa = 3.12NWLNRR259 pKa = 11.84DD260 pKa = 3.55FLAFFNPLVGRR271 pKa = 11.84FLTQRR276 pKa = 11.84PTFARR281 pKa = 11.84MHH283 pKa = 6.69FEE285 pKa = 4.28TEE287 pKa = 3.96VLPIDD292 pKa = 4.02GTGNPYY298 pKa = 8.94TAFLLASDD306 pKa = 4.81EE307 pKa = 4.3NTSLATTVLTAPSTFVNANDD327 pKa = 3.91PKK329 pKa = 10.25SPKK332 pKa = 9.81LGSVLASLSGTLLLNYY348 pKa = 10.03AIEE351 pKa = 4.76PPTLPTWTAATYY363 pKa = 10.32NQDD366 pKa = 4.57DD367 pKa = 4.37NPSDD371 pKa = 3.69ISDD374 pKa = 3.3KK375 pKa = 11.1TFATEE380 pKa = 3.95HH381 pKa = 6.18NFLVDD386 pKa = 3.97EE387 pKa = 4.41STHH390 pKa = 7.07DD391 pKa = 3.45KK392 pKa = 9.91TLSYY396 pKa = 10.79PDD398 pKa = 5.49DD399 pKa = 4.01DD400 pKa = 4.44TGLNAAWYY408 pKa = 9.44AIAKK412 pKa = 8.65VKK414 pKa = 8.41HH415 pKa = 5.33TKK417 pKa = 10.21ARR419 pKa = 11.84TPFKK423 pKa = 10.73HH424 pKa = 6.87VIFNVKK430 pKa = 10.12DD431 pKa = 3.32HH432 pKa = 6.32LTPYY436 pKa = 10.45VLYY439 pKa = 8.92FQPYY443 pKa = 8.43DD444 pKa = 3.69VSPSSLDD451 pKa = 3.26LTIAAGIKK459 pKa = 9.41IEE461 pKa = 4.69HH462 pKa = 6.81GDD464 pKa = 3.17ISGFAINIEE473 pKa = 4.22QPDD476 pKa = 3.78SSLDD480 pKa = 3.69DD481 pKa = 3.73NNAQILQAAIRR492 pKa = 11.84LSKK495 pKa = 10.3VIRR498 pKa = 11.84VNAQTSAGDD507 pKa = 3.56NRR509 pKa = 11.84VSIAVRR515 pKa = 11.84EE516 pKa = 4.04PLDD519 pKa = 3.48RR520 pKa = 11.84TKK522 pKa = 10.89QGVMYY527 pKa = 10.44IFRR530 pKa = 11.84SLLKK534 pKa = 10.61SVFPVLDD541 pKa = 3.8NEE543 pKa = 4.69DD544 pKa = 3.59VKK546 pKa = 11.19STDD549 pKa = 2.93TVLPTNVGLTIEE561 pKa = 4.3RR562 pKa = 11.84GHH564 pKa = 6.48YY565 pKa = 10.86SFDD568 pKa = 2.97QGFNVKK574 pKa = 10.15AGSNGDD580 pKa = 3.34IKK582 pKa = 10.32TSDD585 pKa = 3.34DD586 pKa = 5.2SIYY589 pKa = 10.33LWSSYY594 pKa = 10.72RR595 pKa = 11.84YY596 pKa = 8.02VHH598 pKa = 6.49KK599 pKa = 10.6KK600 pKa = 10.09KK601 pKa = 10.65NPAPADD607 pKa = 3.24ISMLASLRR615 pKa = 11.84PFYY618 pKa = 11.07GNNVTLSRR626 pKa = 11.84SKK628 pKa = 11.12NPTLLIPHH636 pKa = 7.39

Molecular weight: 71.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GHF1|Q5GHF1_9VIRU RNA-dependent RNA polymerase OS=Pleurotus ostreatus virus 1 OX=674983 PE=4 SV=1
MM1 pKa = 7.36SFLRR5 pKa = 11.84IRR7 pKa = 11.84DD8 pKa = 3.73YY9 pKa = 9.7FTEE12 pKa = 4.11RR13 pKa = 11.84LKK15 pKa = 11.09HH16 pKa = 6.71LSRR19 pKa = 11.84DD20 pKa = 3.03WKK22 pKa = 10.52IFQQSDD28 pKa = 3.47SDD30 pKa = 4.31PEE32 pKa = 4.34STLATHH38 pKa = 7.31LDD40 pKa = 3.3SDD42 pKa = 4.13IARR45 pKa = 11.84LEE47 pKa = 4.12HH48 pKa = 6.66GIKK51 pKa = 10.38SSLSDD56 pKa = 3.56DD57 pKa = 3.84QRR59 pKa = 11.84QQAYY63 pKa = 7.92EE64 pKa = 3.77RR65 pKa = 11.84EE66 pKa = 4.34YY67 pKa = 11.57NRR69 pKa = 11.84IHH71 pKa = 7.09SALHH75 pKa = 6.13DD76 pKa = 3.88KK77 pKa = 10.68ARR79 pKa = 11.84QDD81 pKa = 3.44GFPDD85 pKa = 2.95EE86 pKa = 5.17FYY88 pKa = 10.85RR89 pKa = 11.84SRR91 pKa = 11.84SIDD94 pKa = 3.76DD95 pKa = 4.56LPDD98 pKa = 3.2NRR100 pKa = 11.84IPPSGIIPLPYY111 pKa = 9.42EE112 pKa = 3.75YY113 pKa = 10.28HH114 pKa = 6.85RR115 pKa = 11.84SQVVISTEE123 pKa = 4.1EE124 pKa = 3.86VPEE127 pKa = 4.15TGFQIDD133 pKa = 3.67PRR135 pKa = 11.84IVRR138 pKa = 11.84ILRR141 pKa = 11.84NKK143 pKa = 8.39YY144 pKa = 8.56PQYY147 pKa = 10.38LPHH150 pKa = 5.07VTKK153 pKa = 10.7YY154 pKa = 10.38VRR156 pKa = 11.84PLGTTDD162 pKa = 2.98ATVKK166 pKa = 10.8DD167 pKa = 4.24FFKK170 pKa = 10.7PQIPSDD176 pKa = 4.99PIPEE180 pKa = 3.94ARR182 pKa = 11.84KK183 pKa = 9.59QRR185 pKa = 11.84ILDD188 pKa = 3.69LVISFLACTPFLPLHH203 pKa = 6.77FIDD206 pKa = 4.92SLWDD210 pKa = 3.28KK211 pKa = 10.8TPLHH215 pKa = 6.47TGTGYY220 pKa = 10.87FNRR223 pKa = 11.84HH224 pKa = 4.3SFAARR229 pKa = 11.84IHH231 pKa = 6.6AMFSAPRR238 pKa = 11.84LYY240 pKa = 10.58EE241 pKa = 3.7RR242 pKa = 11.84RR243 pKa = 11.84TTSKK247 pKa = 10.94GYY249 pKa = 9.87FINYY253 pKa = 8.87FLEE256 pKa = 4.42TARR259 pKa = 11.84STIHH263 pKa = 6.46NIKK266 pKa = 10.43LHH268 pKa = 6.1GFPFDD273 pKa = 3.74PSKK276 pKa = 11.58VPDD279 pKa = 4.11LGSALRR285 pKa = 11.84SFILKK290 pKa = 10.01RR291 pKa = 11.84PTMLFTRR298 pKa = 11.84NHH300 pKa = 5.58ISDD303 pKa = 3.44RR304 pKa = 11.84DD305 pKa = 3.78GNLKK309 pKa = 9.56QRR311 pKa = 11.84PVYY314 pKa = 11.14AMDD317 pKa = 4.44DD318 pKa = 3.43LFIRR322 pKa = 11.84LEE324 pKa = 4.1SMITFPLHH332 pKa = 5.13VMARR336 pKa = 11.84KK337 pKa = 9.6IEE339 pKa = 4.05CCIMYY344 pKa = 9.9GYY346 pKa = 8.32EE347 pKa = 4.21TIRR350 pKa = 11.84GSNRR354 pKa = 11.84QIDD357 pKa = 4.26KK358 pKa = 9.76IASSFRR364 pKa = 11.84SFFTIDD370 pKa = 2.61WSGFDD375 pKa = 3.15QRR377 pKa = 11.84LPRR380 pKa = 11.84VITDD384 pKa = 3.62IFWSDD389 pKa = 3.2FLEE392 pKa = 4.26RR393 pKa = 11.84MIVISHH399 pKa = 7.01GYY401 pKa = 8.47QPTYY405 pKa = 10.6DD406 pKa = 3.72YY407 pKa = 11.06PSYY410 pKa = 10.97PDD412 pKa = 3.52LTPDD416 pKa = 4.63KK417 pKa = 10.16MFQRR421 pKa = 11.84MDD423 pKa = 3.78NILFFLHH430 pKa = 5.31TWYY433 pKa = 11.09NNLVFVTADD442 pKa = 2.98GFAYY446 pKa = 9.65IRR448 pKa = 11.84TCAGVPSGLLNTQYY462 pKa = 11.45LDD464 pKa = 3.46SFSNLFLIFDD474 pKa = 3.96GLIEE478 pKa = 4.56FGCSDD483 pKa = 3.66AEE485 pKa = 4.06IYY487 pKa = 10.65QIFLLVMGDD496 pKa = 3.81DD497 pKa = 3.92NSAFTLWSIAKK508 pKa = 10.1LEE510 pKa = 4.09EE511 pKa = 3.95FLSFFEE517 pKa = 4.73SYY519 pKa = 10.89ALRR522 pKa = 11.84RR523 pKa = 11.84FGMVLSKK530 pKa = 9.28TKK532 pKa = 10.75SVITVIRR539 pKa = 11.84GKK541 pKa = 9.98IEE543 pKa = 3.88TLSYY547 pKa = 8.71QCNYY551 pKa = 9.43GAPKK555 pKa = 10.23RR556 pKa = 11.84PLAKK560 pKa = 10.21LVAQLCYY567 pKa = 10.0PEE569 pKa = 4.84RR570 pKa = 11.84GPRR573 pKa = 11.84AKK575 pKa = 9.4YY576 pKa = 8.69TSARR580 pKa = 11.84AIGMAYY586 pKa = 9.68AACAMDD592 pKa = 3.87RR593 pKa = 11.84TFHH596 pKa = 6.75DD597 pKa = 4.25LCRR600 pKa = 11.84DD601 pKa = 3.19IYY603 pKa = 11.45YY604 pKa = 10.74EE605 pKa = 4.17FLDD608 pKa = 5.01DD609 pKa = 4.66SASPDD614 pKa = 3.17EE615 pKa = 4.52PFFFEE620 pKa = 5.15HH621 pKa = 5.37VQAYY625 pKa = 10.17LPGILRR631 pKa = 11.84TDD633 pKa = 3.51EE634 pKa = 4.25SLSTQISLSSFPSFLTVQQHH654 pKa = 4.89ISRR657 pKa = 11.84WQGPLSYY664 pKa = 10.45YY665 pKa = 9.65PKK667 pKa = 9.13WDD669 pKa = 3.4RR670 pKa = 11.84AHH672 pKa = 7.35FINDD676 pKa = 3.65PDD678 pKa = 4.36VIPPSAEE685 pKa = 3.34TMAEE689 pKa = 3.88YY690 pKa = 10.32RR691 pKa = 11.84SRR693 pKa = 11.84NSIPRR698 pKa = 11.84RR699 pKa = 11.84DD700 pKa = 4.36IPSLWQQ706 pKa = 3.32

Molecular weight: 82.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1342

636

706

671.0

76.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.855 ± 0.975

1.043 ± 0.17

7.526 ± 0.014

3.577 ± 0.493

6.334 ± 0.652

4.024 ± 0.042

3.204 ± 0.168

6.557 ± 0.903

3.875 ± 0.141

9.538 ± 0.45

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.028

4.471 ± 1.407

6.632 ± 0.019

2.981 ± 0.514

6.185 ± 1.28

8.271 ± 0.27

6.557 ± 0.757

4.396 ± 0.73

1.043 ± 0.17

4.844 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski