Tranquillimonas sp. M0103

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Mesobaculum

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3564 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A438AJ36|A0A438AJ36_9RHOB DeoR/GlpR transcriptional regulator OS=Tranquillimonas sp. M0103 OX=2486419 GN=EKE94_07305 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84LISLSTLAALAACPVAADD20 pKa = 4.15EE21 pKa = 5.76LDD23 pKa = 3.8TQAVALFEE31 pKa = 4.65AAFSDD36 pKa = 5.31DD37 pKa = 3.29ICPLYY42 pKa = 10.66FDD44 pKa = 5.71DD45 pKa = 4.72PAAPEE50 pKa = 4.48RR51 pKa = 11.84YY52 pKa = 9.78DD53 pKa = 3.12ITRR56 pKa = 11.84GEE58 pKa = 4.5GEE60 pKa = 3.97GTLTVFAFTCDD71 pKa = 3.35FGAYY75 pKa = 9.54NRR77 pKa = 11.84VDD79 pKa = 3.02AFVIQTNLDD88 pKa = 3.76GLHH91 pKa = 5.86VASFAQPALDD101 pKa = 3.86LTYY104 pKa = 10.51TDD106 pKa = 4.7EE107 pKa = 4.43NNTMEE112 pKa = 5.22SEE114 pKa = 4.11LAHH117 pKa = 6.59LGIAGYY123 pKa = 7.6EE124 pKa = 4.16TTATLINAAVDD135 pKa = 3.49EE136 pKa = 4.43RR137 pKa = 11.84TGQIVTQEE145 pKa = 3.66KK146 pKa = 8.91WRR148 pKa = 11.84GVGDD152 pKa = 3.76AMSAGTWDD160 pKa = 3.99LTLQGYY166 pKa = 7.28VLRR169 pKa = 11.84DD170 pKa = 3.63YY171 pKa = 11.08EE172 pKa = 4.26VDD174 pKa = 3.13ASYY177 pKa = 11.19DD178 pKa = 3.59GEE180 pKa = 4.56LNPVSLLKK188 pKa = 10.79NGQPVDD194 pKa = 3.32

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A438AEH9|A0A438AEH9_9RHOB Uncharacterized protein OS=Tranquillimonas sp. M0103 OX=2486419 GN=EKE94_15540 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3564

0

3564

1129772

26

1935

317.0

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.329 ± 0.067

0.824 ± 0.011

6.408 ± 0.035

5.776 ± 0.037

3.452 ± 0.026

9.194 ± 0.043

1.997 ± 0.023

4.701 ± 0.029

2.29 ± 0.033

10.115 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.019

2.077 ± 0.018

5.602 ± 0.037

2.976 ± 0.019

7.617 ± 0.04

4.871 ± 0.027

5.526 ± 0.027

7.202 ± 0.028

1.396 ± 0.019

2.063 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski