Pea leaf distortion virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8JPK8|A0A1P8JPK8_9GEMI Protein V2 OS=Pea leaf distortion virus OX=1931111 GN=V2 PE=3 SV=1
MM1 pKa = 7.31APKK4 pKa = 10.4RR5 pKa = 11.84FVIYY9 pKa = 10.27SKK11 pKa = 11.13NYY13 pKa = 7.97FLTYY17 pKa = 9.19PKK19 pKa = 10.53CSLTKK24 pKa = 10.66EE25 pKa = 4.11EE26 pKa = 5.14ALSQIQNLQTPTSQKK41 pKa = 9.77YY42 pKa = 10.02IKK44 pKa = 9.7ICRR47 pKa = 11.84EE48 pKa = 3.53LHH50 pKa = 6.26EE51 pKa = 4.93NGEE54 pKa = 4.28PHH56 pKa = 6.66LHH58 pKa = 6.03VLIQFEE64 pKa = 4.78GKK66 pKa = 9.95YY67 pKa = 9.77KK68 pKa = 10.46CQNQRR73 pKa = 11.84FFDD76 pKa = 3.9LVSPNRR82 pKa = 11.84SAHH85 pKa = 5.06FHH87 pKa = 6.56PNIQGAKK94 pKa = 9.25SSSDD98 pKa = 3.11VKK100 pKa = 11.24SYY102 pKa = 10.79IDD104 pKa = 3.7KK105 pKa = 11.33DD106 pKa = 3.49GDD108 pKa = 3.67TLEE111 pKa = 4.14WGEE114 pKa = 3.84FQIDD118 pKa = 3.21GRR120 pKa = 11.84SARR123 pKa = 11.84GGQQTANDD131 pKa = 4.05AYY133 pKa = 10.69AAALNAGSKK142 pKa = 10.29SEE144 pKa = 3.86ALRR147 pKa = 11.84VIRR150 pKa = 11.84EE151 pKa = 4.09LAPKK155 pKa = 10.52DD156 pKa = 3.64FVLQFHH162 pKa = 6.7NLNANLDD169 pKa = 4.29RR170 pKa = 11.84IFQEE174 pKa = 4.24PPAPYY179 pKa = 9.91VSPFSSSSFDD189 pKa = 3.41QVPEE193 pKa = 3.95EE194 pKa = 4.04LEE196 pKa = 3.96EE197 pKa = 4.17WVSEE201 pKa = 4.2NVVDD205 pKa = 4.8AAARR209 pKa = 11.84PLRR212 pKa = 11.84PQSIVIEE219 pKa = 4.44GDD221 pKa = 3.12SRR223 pKa = 11.84TGKK226 pKa = 8.52TMWARR231 pKa = 11.84SLGPHH236 pKa = 6.71NYY238 pKa = 10.18LCGHH242 pKa = 7.38LDD244 pKa = 4.18LSPKK248 pKa = 10.15VYY250 pKa = 11.07SNDD253 pKa = 2.25AWYY256 pKa = 10.92NVIDD260 pKa = 4.79DD261 pKa = 4.33VDD263 pKa = 3.73PHH265 pKa = 6.08FLKK268 pKa = 10.67HH269 pKa = 5.9FKK271 pKa = 10.69EE272 pKa = 4.43FMGAQRR278 pKa = 11.84DD279 pKa = 3.81WQSNTKK285 pKa = 9.6YY286 pKa = 10.57GKK288 pKa = 9.12PVQIKK293 pKa = 10.41GGIPTIFLCNPGPNSSYY310 pKa = 11.34KK311 pKa = 10.62EE312 pKa = 3.85FLDD315 pKa = 3.82EE316 pKa = 4.66EE317 pKa = 4.61KK318 pKa = 11.18NSALKK323 pKa = 10.53NWALKK328 pKa = 10.11NAIFITLEE336 pKa = 4.08GPLYY340 pKa = 10.77SGSNQSTAQGSEE352 pKa = 3.9EE353 pKa = 3.88AQQEE357 pKa = 4.37EE358 pKa = 4.97EE359 pKa = 4.31SRR361 pKa = 11.84SS362 pKa = 3.6

Molecular weight: 40.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8JPJ2|A0A1P8JPJ2_9GEMI Replication-associated protein OS=Pea leaf distortion virus OX=1931111 GN=Rep PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.32VRR18 pKa = 11.84HH19 pKa = 5.85RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.88ACRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.63SRR42 pKa = 11.84MWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.39EE124 pKa = 4.08NIKK127 pKa = 10.76SKK129 pKa = 10.6NHH131 pKa = 5.54TNNVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.77QVLRR180 pKa = 11.84KK181 pKa = 9.41WYY183 pKa = 8.08ATVTGGQYY191 pKa = 9.96GAKK194 pKa = 9.62EE195 pKa = 3.57QALVKK200 pKa = 10.7KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.08YY219 pKa = 8.01EE220 pKa = 4.07NHH222 pKa = 6.29SEE224 pKa = 4.11NALMLYY230 pKa = 7.58MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1119

102

362

186.5

21.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.183 ± 0.924

2.234 ± 0.539

4.915 ± 0.297

5.094 ± 0.981

4.021 ± 0.429

5.004 ± 0.34

3.753 ± 0.723

5.004 ± 0.776

5.63 ± 0.863

7.239 ± 1.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.699

5.63 ± 0.544

5.898 ± 0.532

5.541 ± 0.902

6.613 ± 0.997

9.383 ± 1.584

5.004 ± 1.105

5.987 ± 1.536

1.519 ± 0.209

4.111 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski