Lacipirellula limnantheis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Lacipirellula

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517U649|A0A517U649_9BACT Uncharacterized protein OS=Lacipirellula limnantheis OX=2528024 GN=I41_53530 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 5.33KK3 pKa = 10.79YY4 pKa = 11.18QPLRR8 pKa = 11.84DD9 pKa = 3.72ADD11 pKa = 4.3TNGANYY17 pKa = 10.57DD18 pKa = 3.79LDD20 pKa = 4.92ADD22 pKa = 4.04QIIAQLQTWDD32 pKa = 3.19AKK34 pKa = 11.17YY35 pKa = 10.71GVTLSDD41 pKa = 3.54VAHH44 pKa = 7.08DD45 pKa = 3.7AVTVTFNAIPVDD57 pKa = 4.13DD58 pKa = 4.7VPALAAEE65 pKa = 4.49IYY67 pKa = 8.66EE68 pKa = 4.27FCPDD72 pKa = 4.13TIDD75 pKa = 3.45QHH77 pKa = 6.46FGCFAEE83 pKa = 4.62MIEE86 pKa = 4.44MADD89 pKa = 3.48EE90 pKa = 4.49TGEE93 pKa = 4.04EE94 pKa = 4.49LPPEE98 pKa = 4.34LLEE101 pKa = 4.3LTTGVDD107 pKa = 5.24FEE109 pKa = 4.72DD110 pKa = 3.62EE111 pKa = 4.37QYY113 pKa = 11.64GLEE116 pKa = 3.96LLQRR120 pKa = 11.84SLAKK124 pKa = 10.06HH125 pKa = 5.34RR126 pKa = 11.84QVALWWDD133 pKa = 3.36

Molecular weight: 15.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517TYJ6|A0A517TYJ6_9BACT Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol mannoside mannosyltransferase OS=Lacipirellula limnantheis OX=2528024 GN=I41_26310 PE=3 SV=1
MM1 pKa = 7.46YY2 pKa = 10.46SPTCHH7 pKa = 6.37SSRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84VLGSSKK18 pKa = 10.6ACHH21 pKa = 5.74QPVEE25 pKa = 4.06LLARR29 pKa = 11.84RR30 pKa = 11.84VVNGARR36 pKa = 11.84IVQRR40 pKa = 11.84PGSSYY45 pKa = 10.76HH46 pKa = 6.32HH47 pKa = 7.2APLPADD53 pKa = 3.44SRR55 pKa = 11.84PVGRR59 pKa = 11.84LALPKK64 pKa = 10.35GDD66 pKa = 3.49TAIDD70 pKa = 3.51QEE72 pKa = 4.34EE73 pKa = 4.3AVAVMVEE80 pKa = 4.29KK81 pKa = 10.43AASISTFEE89 pKa = 4.12GKK91 pKa = 9.77VRR93 pKa = 11.84LRR95 pKa = 11.84WYY97 pKa = 10.66VKK99 pKa = 10.32LQTPEE104 pKa = 3.44ARR106 pKa = 11.84IMLRR110 pKa = 11.84CLRR113 pKa = 11.84LTAAFTCIMLCSVFIVFWMRR133 pKa = 11.84SYY135 pKa = 11.04KK136 pKa = 10.32VQDD139 pKa = 3.88GVTVWYY145 pKa = 8.38ATGKK149 pKa = 7.88TVAFGRR155 pKa = 11.84NEE157 pKa = 3.62QRR159 pKa = 11.84PLISRR164 pKa = 11.84VIVGSTNGTAAVRR177 pKa = 11.84WAAEE181 pKa = 4.3HH182 pKa = 6.12VFSGTPEE189 pKa = 3.81RR190 pKa = 11.84VRR192 pKa = 11.84VEE194 pKa = 3.82FLRR197 pKa = 11.84DD198 pKa = 3.19GGYY201 pKa = 10.73DD202 pKa = 3.57SQWAFKK208 pKa = 10.01KK209 pKa = 10.5RR210 pKa = 11.84SGFAFSEE217 pKa = 4.13NNEE220 pKa = 3.97RR221 pKa = 11.84AVQVAAPYY229 pKa = 9.95WFVTLVTGTAGTLLWMNRR247 pKa = 11.84PYY249 pKa = 11.06RR250 pKa = 11.84FTLRR254 pKa = 11.84GSLAVTTLIALVLGASVVFSRR275 pKa = 5.25

Molecular weight: 30.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5493

0

5493

1881987

29

3101

342.6

37.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.005 ± 0.044

1.065 ± 0.016

5.747 ± 0.026

5.864 ± 0.038

3.631 ± 0.02

8.381 ± 0.051

2.038 ± 0.015

4.531 ± 0.024

3.144 ± 0.031

9.871 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.015

3.078 ± 0.03

5.431 ± 0.028

3.751 ± 0.025

6.833 ± 0.033

5.927 ± 0.03

5.39 ± 0.033

7.285 ± 0.026

1.578 ± 0.016

2.365 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski