Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b)

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Leptotrichiaceae; Leptotrichia; Leptotrichia buccalis

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7N9L2|C7N9L2_LEPBD Uncharacterized protein OS=Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b) OX=523794 GN=Lebu_0940 PE=4 SV=1
MM1 pKa = 6.85LQWYY5 pKa = 9.31GEE7 pKa = 4.19QIDD10 pKa = 4.86IIDD13 pKa = 3.76NLYY16 pKa = 10.41EE17 pKa = 4.03FLSKK21 pKa = 10.9RR22 pKa = 11.84EE23 pKa = 4.13DD24 pKa = 3.55DD25 pKa = 4.82DD26 pKa = 5.72EE27 pKa = 5.08ILEE30 pKa = 5.19FIWEE34 pKa = 4.11NKK36 pKa = 8.73NIKK39 pKa = 9.87IKK41 pKa = 10.52GRR43 pKa = 11.84YY44 pKa = 8.72LEE46 pKa = 4.14QSEE49 pKa = 4.78SNYY52 pKa = 9.82NSEE55 pKa = 4.04YY56 pKa = 11.12AEE58 pKa = 4.85GYY60 pKa = 9.56FDD62 pKa = 4.41NIKK65 pKa = 10.52EE66 pKa = 4.16IGFEE70 pKa = 4.2GYY72 pKa = 9.56IYY74 pKa = 10.67EE75 pKa = 4.3VLSEE79 pKa = 5.37VKK81 pKa = 10.76DD82 pKa = 3.4EE83 pKa = 4.16WLDD86 pKa = 3.52EE87 pKa = 4.11NFIGRR92 pKa = 11.84DD93 pKa = 3.31SEE95 pKa = 4.56GVITAFISVYY105 pKa = 10.21YY106 pKa = 9.39YY107 pKa = 10.23PNEE110 pKa = 3.52IRR112 pKa = 11.84NFF114 pKa = 3.55

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7NEN6|C7NEN6_LEPBD Uncharacterized protein OS=Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b) OX=523794 GN=Lebu_0486 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.09RR4 pKa = 11.84TYY6 pKa = 10.42QPNKK10 pKa = 9.53RR11 pKa = 11.84KK12 pKa = 9.8RR13 pKa = 11.84KK14 pKa = 8.43KK15 pKa = 9.19NHH17 pKa = 5.02GFRR20 pKa = 11.84KK21 pKa = 10.09RR22 pKa = 11.84MQDD25 pKa = 2.9KK26 pKa = 10.68NGRR29 pKa = 11.84NVLKK33 pKa = 10.49RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.53GRR40 pKa = 11.84AKK42 pKa = 10.69LSAA45 pKa = 3.92

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2218

0

2218

684996

31

3644

308.8

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.26 ± 0.068

0.664 ± 0.017

5.319 ± 0.033

8.44 ± 0.079

4.974 ± 0.049

6.204 ± 0.078

1.218 ± 0.021

9.35 ± 0.064

10.566 ± 0.078

8.752 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.025

6.813 ± 0.058

2.475 ± 0.025

2.418 ± 0.024

3.474 ± 0.033

5.664 ± 0.043

4.814 ± 0.061

6.145 ± 0.047

0.688 ± 0.016

4.307 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski