Helicobacter phage KHP40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; Helicobacter virus KHP40

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I7H0J2|I7H0J2_9CAUD Uncharacterized protein OS=Helicobacter phage KHP40 OX=1204178 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 9.99FLEE5 pKa = 4.11MLAISVLIFILGISFILAVYY25 pKa = 9.0FSVEE29 pKa = 3.91ALCYY33 pKa = 10.24GG34 pKa = 3.7

Molecular weight:
3.82 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I7GUT4|I7GUT4_9CAUD ddrB-ParB domain-containing protein OS=Helicobacter phage KHP40 OX=1204178 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84NYY4 pKa = 10.46KK5 pKa = 9.79HH6 pKa = 6.59ANKK9 pKa = 9.99NFKK12 pKa = 10.04PKK14 pKa = 9.66VWTNSVLLEE23 pKa = 3.99NGILEE28 pKa = 4.07ILEE31 pKa = 3.99ALKK34 pKa = 9.99TIEE37 pKa = 4.05NRR39 pKa = 11.84SRR41 pKa = 11.84SQVLEE46 pKa = 3.58RR47 pKa = 11.84LIIFFIEE54 pKa = 4.3TQKK57 pKa = 10.96GQSNEE62 pKa = 4.23MAWKK66 pKa = 10.1RR67 pKa = 11.84SQRR70 pKa = 11.84AYY72 pKa = 10.19KK73 pKa = 9.34RR74 pKa = 11.84TLTNKK79 pKa = 8.22TEE81 pKa = 4.07KK82 pKa = 10.72NKK84 pKa = 10.46LKK86 pKa = 10.44RR87 pKa = 11.84KK88 pKa = 7.37QFEE91 pKa = 4.52RR92 pKa = 11.84IEE94 pKa = 4.02KK95 pKa = 7.2TQKK98 pKa = 10.45KK99 pKa = 9.41KK100 pKa = 10.55QLQAGANYY108 pKa = 8.81PFSFFEE114 pKa = 4.23RR115 pKa = 11.84AA116 pKa = 3.06

Molecular weight:
13.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

8251

34

1725

257.8

29.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.799 ± 0.373

0.788 ± 0.254

5.308 ± 0.381

8.205 ± 0.389

4.715 ± 0.287

4.048 ± 0.26

1.454 ± 0.164

6.92 ± 0.282

10.896 ± 0.443

10.496 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.197

8.193 ± 0.443

2.485 ± 0.176

4.545 ± 0.338

4.072 ± 0.266

6.472 ± 0.322

4.569 ± 0.244

4.084 ± 0.432

0.545 ± 0.115

3.636 ± 0.244

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski