[Clostridium] spiroforme DSM 1552

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium; [Clostridium] spiroforme

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1C435|B1C435_9FIRM Uncharacterized protein OS=[Clostridium] spiroforme DSM 1552 OX=428126 GN=CLOSPI_02003 PE=4 SV=1
MM1 pKa = 7.22NKK3 pKa = 10.1KK4 pKa = 10.62LIILVIALIIVVVTGISIYY23 pKa = 9.92TLSKK27 pKa = 11.13DD28 pKa = 4.43DD29 pKa = 6.11DD30 pKa = 4.01NTTTANQQSDD40 pKa = 3.67NTSVEE45 pKa = 3.94NNKK48 pKa = 9.87NDD50 pKa = 3.75LEE52 pKa = 4.97AGNVLIVYY60 pKa = 9.42FSEE63 pKa = 4.12TGNTEE68 pKa = 3.69TVANIIHH75 pKa = 7.11DD76 pKa = 4.1NVGGDD81 pKa = 3.23IVKK84 pKa = 10.84LEE86 pKa = 4.15TTNAYY91 pKa = 8.73PSNYY95 pKa = 10.0DD96 pKa = 3.38EE97 pKa = 4.95LVDD100 pKa = 4.08YY101 pKa = 10.11AQQEE105 pKa = 4.42QQDD108 pKa = 3.84DD109 pKa = 3.94ARR111 pKa = 11.84PEE113 pKa = 3.77LSTAIEE119 pKa = 4.44NIEE122 pKa = 3.98QYY124 pKa = 10.02DD125 pKa = 4.38TIFLGYY131 pKa = 8.52PNWWGDD137 pKa = 3.59MPMALYY143 pKa = 9.76TFLDD147 pKa = 4.07TYY149 pKa = 11.21DD150 pKa = 4.47LSGKK154 pKa = 8.54TIAPFITHH162 pKa = 6.91GGSGLSGTPEE172 pKa = 4.15NIQEE176 pKa = 4.21EE177 pKa = 4.77EE178 pKa = 3.95PNAIVTEE185 pKa = 4.14GLAIDD190 pKa = 4.51GDD192 pKa = 4.07EE193 pKa = 4.45ASYY196 pKa = 11.05SSKK199 pKa = 11.26DD200 pKa = 3.38VVEE203 pKa = 4.32WLNSLEE209 pKa = 4.11FF210 pKa = 4.79

Molecular weight: 23.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1C5J8|B1C5J8_9FIRM Zn_dep_PLPC domain-containing protein OS=[Clostridium] spiroforme DSM 1552 OX=428126 GN=CLOSPI_02427 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2358

0

2358

715586

39

2169

303.5

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.634 ± 0.063

1.3 ± 0.022

6.196 ± 0.045

6.652 ± 0.047

4.296 ± 0.039

5.886 ± 0.05

1.667 ± 0.02

9.537 ± 0.081

8.511 ± 0.053

9.248 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.024

6.468 ± 0.046

2.68 ± 0.024

3.038 ± 0.029

3.185 ± 0.031

5.941 ± 0.037

5.131 ± 0.05

6.456 ± 0.04

0.683 ± 0.018

4.791 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski