Verrucomicrobiaceae bacterium SCGC AG-212-N21

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; unclassified Verrucomicrobiaceae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177RF53|A0A177RF53_9BACT SHSP domain-containing protein OS=Verrucomicrobiaceae bacterium SCGC AG-212-N21 OX=1799659 GN=AYO49_06305 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.59LILHH6 pKa = 6.86LLFASALITSASAGTYY22 pKa = 8.4TSSKK26 pKa = 8.71SAKK29 pKa = 9.84AVVPPAPLGCDD40 pKa = 3.18CFAPGFAIGIFGSGVLFNEE59 pKa = 5.07DD60 pKa = 5.49DD61 pKa = 4.07IDD63 pKa = 5.19DD64 pKa = 4.75DD65 pKa = 4.38SLGGGVLAEE74 pKa = 4.11YY75 pKa = 10.31FFTEE79 pKa = 4.25NIGIQGSYY87 pKa = 11.09GIFATEE93 pKa = 4.13KK94 pKa = 9.74EE95 pKa = 4.24LHH97 pKa = 6.48EE98 pKa = 4.51FDD100 pKa = 3.57AALVLRR106 pKa = 11.84YY107 pKa = 9.11PITSLCLAPYY117 pKa = 10.25IMGGAGYY124 pKa = 8.03STNSQDD130 pKa = 3.47AWNLFLGGGIDD141 pKa = 3.83VRR143 pKa = 11.84CPEE146 pKa = 4.41WNCVSLFADD155 pKa = 5.06GAYY158 pKa = 9.7HH159 pKa = 7.18WSEE162 pKa = 5.23DD163 pKa = 3.65DD164 pKa = 4.7DD165 pKa = 6.44ADD167 pKa = 3.8FTIVRR172 pKa = 11.84IGLKK176 pKa = 10.38FPLL179 pKa = 4.39

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177RAJ0|A0A177RAJ0_9BACT Uncharacterized protein OS=Verrucomicrobiaceae bacterium SCGC AG-212-N21 OX=1799659 GN=AYO49_04755 PE=4 SV=1
MM1 pKa = 7.76PMQHH5 pKa = 6.35EE6 pKa = 5.21AIQLEE11 pKa = 4.42AGQSFRR17 pKa = 11.84LLHH20 pKa = 6.12WKK22 pKa = 10.51DD23 pKa = 3.3NLRR26 pKa = 11.84EE27 pKa = 3.92VDD29 pKa = 3.76SLIGPEE35 pKa = 3.88EE36 pKa = 3.94RR37 pKa = 11.84VRR39 pKa = 11.84IHH41 pKa = 6.38GAGDD45 pKa = 3.06RR46 pKa = 11.84WHH48 pKa = 6.84HH49 pKa = 5.47HH50 pKa = 6.46RR51 pKa = 11.84EE52 pKa = 3.78MEE54 pKa = 4.1LTVVQRR60 pKa = 11.84GSGTRR65 pKa = 11.84FVGDD69 pKa = 3.73HH70 pKa = 6.64IGPFEE75 pKa = 4.28SLDD78 pKa = 3.59VVLIGANVPHH88 pKa = 6.97FWKK91 pKa = 10.66GMRR94 pKa = 11.84KK95 pKa = 8.71SSGYY99 pKa = 8.5AVQWRR104 pKa = 11.84FEE106 pKa = 4.18RR107 pKa = 11.84EE108 pKa = 3.41HH109 pKa = 7.3ALWSFPEE116 pKa = 4.22SGALRR121 pKa = 11.84VLWEE125 pKa = 4.11RR126 pKa = 11.84AAHH129 pKa = 6.02GLRR132 pKa = 11.84FTGATADD139 pKa = 3.77RR140 pKa = 11.84ALALVQSMARR150 pKa = 11.84RR151 pKa = 11.84EE152 pKa = 4.42GCGRR156 pKa = 11.84LAMLFEE162 pKa = 5.24LLDD165 pKa = 4.01KK166 pKa = 10.95LATAPRR172 pKa = 11.84RR173 pKa = 11.84EE174 pKa = 4.18CQKK177 pKa = 10.65LSQRR181 pKa = 11.84PFDD184 pKa = 3.9LSGAHH189 pKa = 5.95AHH191 pKa = 5.62QPAIEE196 pKa = 3.98RR197 pKa = 11.84VIRR200 pKa = 11.84YY201 pKa = 8.57ILQHH205 pKa = 5.5FRR207 pKa = 11.84EE208 pKa = 5.15PIALAEE214 pKa = 4.16VLRR217 pKa = 11.84LAGMSKK223 pKa = 9.24ATFARR228 pKa = 11.84QFRR231 pKa = 11.84RR232 pKa = 11.84HH233 pKa = 5.87AGRR236 pKa = 11.84PFSAFVNQVRR246 pKa = 11.84LDD248 pKa = 3.57HH249 pKa = 6.84ACRR252 pKa = 11.84EE253 pKa = 4.34LVGSGMSVSEE263 pKa = 4.03AAFASGFNSLSYY275 pKa = 10.34FNRR278 pKa = 11.84AFRR281 pKa = 11.84SAHH284 pKa = 5.16RR285 pKa = 11.84CSPKK289 pKa = 9.68EE290 pKa = 3.72YY291 pKa = 9.49RR292 pKa = 11.84RR293 pKa = 11.84KK294 pKa = 10.49ARR296 pKa = 11.84VSLL299 pKa = 3.86

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1135

0

1135

384530

57

2303

338.8

37.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.721 ± 0.086

1.068 ± 0.023

5.424 ± 0.052

5.886 ± 0.066

3.947 ± 0.045

8.143 ± 0.094

2.411 ± 0.038

4.442 ± 0.047

4.556 ± 0.072

9.85 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.037

3.066 ± 0.059

5.481 ± 0.061

3.427 ± 0.045

6.329 ± 0.07

5.845 ± 0.068

5.74 ± 0.085

7.199 ± 0.062

1.702 ± 0.035

2.385 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski