Butyrivibrio sp. INlla21

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7FCH3|A0A1I7FCH3_9FIRM Uncharacterized protein OS=Butyrivibrio sp. INlla21 OX=1520811 GN=SAMN02910342_00139 PE=4 SV=1
MM1 pKa = 7.06KK2 pKa = 9.7TINFNSVSFNLVNDD16 pKa = 4.34LNVDD20 pKa = 4.28LTRR23 pKa = 11.84LQATIYY29 pKa = 9.03PSNYY33 pKa = 8.68TMEE36 pKa = 4.51QIVASTKK43 pKa = 9.69DD44 pKa = 3.37CNIIKK49 pKa = 10.42VLDD52 pKa = 4.18DD53 pKa = 3.91GKK55 pKa = 10.82IVGQYY60 pKa = 8.92TGYY63 pKa = 7.75TQPLAYY69 pKa = 8.81TMNYY73 pKa = 8.67IDD75 pKa = 5.12SEE77 pKa = 4.84TPVISIEE84 pKa = 4.18LGNADD89 pKa = 5.43VITQLNSLNDD99 pKa = 3.65QISAQAEE106 pKa = 4.45TINALSSVVSDD117 pKa = 3.4VDD119 pKa = 3.82DD120 pKa = 4.38AVAMLASVIGGEE132 pKa = 4.28NNDD135 pKa = 3.44

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7HVB1|A0A1I7HVB1_9FIRM Alpha-glucosidase OS=Butyrivibrio sp. INlla21 OX=1520811 GN=SAMN02910342_01233 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3162

0

3162

1012116

39

2948

320.1

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.374 ± 0.053

1.354 ± 0.016

6.6 ± 0.036

7.398 ± 0.043

4.312 ± 0.033

6.923 ± 0.036

1.577 ± 0.02

7.818 ± 0.04

7.506 ± 0.04

8.338 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.02

4.806 ± 0.029

3.096 ± 0.023

2.526 ± 0.025

3.887 ± 0.028

6.163 ± 0.034

5.271 ± 0.033

6.829 ± 0.03

0.843 ± 0.015

4.387 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski