Phormidium virus WMP4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Wumpquatrovirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0GBV3|Q0GBV3_9CAUD PfWMP4_13 OS=Phormidium virus WMP4 OX=400567 PE=4 SV=1
MM1 pKa = 7.54NEE3 pKa = 3.47QDD5 pKa = 3.41EE6 pKa = 4.86YY7 pKa = 11.72GVFANEE13 pKa = 4.91DD14 pKa = 3.07AWSLINEE21 pKa = 4.54WLRR24 pKa = 11.84EE25 pKa = 4.13CGSTEE30 pKa = 3.48RR31 pKa = 11.84FTPHH35 pKa = 6.94KK36 pKa = 10.19SNLSGYY42 pKa = 9.57EE43 pKa = 3.63VLTIAKK49 pKa = 9.43ACVYY53 pKa = 10.43YY54 pKa = 10.58DD55 pKa = 3.62YY56 pKa = 11.13DD57 pKa = 3.97IDD59 pKa = 4.68DD60 pKa = 5.33FINFGEE66 pKa = 4.24LL67 pKa = 3.14

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0GBS5|Q0GBS5_9CAUD PfWMP4_41 OS=Phormidium virus WMP4 OX=400567 PE=4 SV=1
MM1 pKa = 7.72SKK3 pKa = 10.38RR4 pKa = 11.84SQPQVVYY11 pKa = 10.96APMPTPTQLPQQSPEE26 pKa = 3.69QAQFLTTMQQQMQKK40 pKa = 8.19MQQSYY45 pKa = 8.73DD46 pKa = 3.46QQVRR50 pKa = 11.84GLNEE54 pKa = 3.75QYY56 pKa = 10.69SANSSSTSSILAQLQQQLASQQQLSQQAATEE87 pKa = 3.96LAKK90 pKa = 10.79ARR92 pKa = 11.84EE93 pKa = 4.09SSAAQLSLMEE103 pKa = 4.64GMRR106 pKa = 11.84NMQQGQLADD115 pKa = 3.78ARR117 pKa = 11.84QEE119 pKa = 4.2QNNLTSSLLGRR130 pKa = 11.84MSRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84QARR138 pKa = 11.84QTIYY142 pKa = 10.83SAA144 pKa = 3.62

Molecular weight: 16.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12160

40

1459

270.2

30.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.956 ± 0.506

0.683 ± 0.119

6.028 ± 0.202

5.872 ± 0.321

3.347 ± 0.223

6.587 ± 0.241

1.563 ± 0.155

4.729 ± 0.179

4.836 ± 0.299

9.211 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.233

4.35 ± 0.276

5.123 ± 0.286

5.452 ± 0.43

5.592 ± 0.255

6.472 ± 0.24

6.554 ± 0.313

6.768 ± 0.272

1.414 ± 0.124

3.775 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski