Amino acid dipepetide frequency for Tuber melanosporum (strain Mel28) (Perigord black truffle)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.646AlaAla: 7.646 ± 0.079
0.907AlaCys: 0.907 ± 0.016
3.508AlaAsp: 3.508 ± 0.032
5.031AlaGlu: 5.031 ± 0.053
2.768AlaPhe: 2.768 ± 0.035
5.925AlaGly: 5.925 ± 0.05
1.63AlaHis: 1.63 ± 0.027
4.078AlaIle: 4.078 ± 0.036
4.084AlaLys: 4.084 ± 0.042
7.261AlaLeu: 7.261 ± 0.055
1.734AlaMet: 1.734 ± 0.024
2.647AlaAsn: 2.647 ± 0.03
4.716AlaPro: 4.716 ± 0.057
2.79AlaGln: 2.79 ± 0.038
4.79AlaArg: 4.79 ± 0.047
6.501AlaSer: 6.501 ± 0.056
4.859AlaThr: 4.859 ± 0.047
5.151AlaVal: 5.151 ± 0.04
0.931AlaTrp: 0.931 ± 0.021
1.897AlaTyr: 1.897 ± 0.027
0.002AlaXaa: 0.002 ± 0.001
Cys
0.889CysAla: 0.889 ± 0.015
0.257CysCys: 0.257 ± 0.009
0.614CysAsp: 0.614 ± 0.015
0.647CysGlu: 0.647 ± 0.014
0.545CysPhe: 0.545 ± 0.013
1.021CysGly: 1.021 ± 0.023
0.302CysHis: 0.302 ± 0.011
0.645CysIle: 0.645 ± 0.014
0.538CysLys: 0.538 ± 0.013
1.224CysLeu: 1.224 ± 0.02
0.28CysMet: 0.28 ± 0.01
0.395CysAsn: 0.395 ± 0.01
0.675CysPro: 0.675 ± 0.016
0.378CysGln: 0.378 ± 0.01
0.756CysArg: 0.756 ± 0.017
0.933CysSer: 0.933 ± 0.02
0.674CysThr: 0.674 ± 0.015
0.791CysVal: 0.791 ± 0.015
0.19CysTrp: 0.19 ± 0.007
0.366CysTyr: 0.366 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
3.726AspAla: 3.726 ± 0.038
0.606AspCys: 0.606 ± 0.013
3.755AspAsp: 3.755 ± 0.049
4.368AspGlu: 4.368 ± 0.043
2.009AspPhe: 2.009 ± 0.025
4.131AspGly: 4.131 ± 0.04
1.056AspHis: 1.056 ± 0.022
2.914AspIle: 2.914 ± 0.034
2.342AspLys: 2.342 ± 0.03
4.681AspLeu: 4.681 ± 0.038
1.165AspMet: 1.165 ± 0.019
1.796AspAsn: 1.796 ± 0.024
3.123AspPro: 3.123 ± 0.037
1.528AspGln: 1.528 ± 0.024
2.947AspArg: 2.947 ± 0.037
3.84AspSer: 3.84 ± 0.038
2.718AspThr: 2.718 ± 0.036
3.413AspVal: 3.413 ± 0.036
0.756AspTrp: 0.756 ± 0.018
1.533AspTyr: 1.533 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.26GluAla: 5.26 ± 0.062
0.702GluCys: 0.702 ± 0.017
4.313GluAsp: 4.313 ± 0.047
6.576GluGlu: 6.576 ± 0.08
2.141GluPhe: 2.141 ± 0.029
4.817GluGly: 4.817 ± 0.046
1.27GluHis: 1.27 ± 0.018
3.376GluIle: 3.376 ± 0.035
4.444GluLys: 4.444 ± 0.05
5.411GluLeu: 5.411 ± 0.047
1.637GluMet: 1.637 ± 0.021
2.43GluAsn: 2.43 ± 0.028
2.528GluPro: 2.528 ± 0.035
2.123GluGln: 2.123 ± 0.03
4.475GluArg: 4.475 ± 0.05
4.179GluSer: 4.179 ± 0.042
3.253GluThr: 3.253 ± 0.037
4.152GluVal: 4.152 ± 0.041
0.884GluTrp: 0.884 ± 0.018
1.756GluTyr: 1.756 ± 0.025
0.002GluXaa: 0.002 ± 0.001
Phe
2.763PheAla: 2.763 ± 0.036
0.576PheCys: 0.576 ± 0.015
2.052PheAsp: 2.052 ± 0.027
2.156PheGlu: 2.156 ± 0.026
1.647PhePhe: 1.647 ± 0.029
2.848PheGly: 2.848 ± 0.046
0.864PheHis: 0.864 ± 0.017
1.717PheIle: 1.717 ± 0.029
1.552PheLys: 1.552 ± 0.025
3.615PheLeu: 3.615 ± 0.04
0.756PheMet: 0.756 ± 0.015
1.293PheAsn: 1.293 ± 0.019
2.039PhePro: 2.039 ± 0.027
1.22PheGln: 1.22 ± 0.022
2.01PheArg: 2.01 ± 0.026
3.124PheSer: 3.124 ± 0.034
2.116PheThr: 2.116 ± 0.028
2.398PheVal: 2.398 ± 0.031
0.548PheTrp: 0.548 ± 0.012
1.096PheTyr: 1.096 ± 0.021
0.001PheXaa: 0.001 ± 0.0
Gly
5.416GlyAla: 5.416 ± 0.046
0.901GlyCys: 0.901 ± 0.018
3.971GlyAsp: 3.971 ± 0.037
4.773GlyGlu: 4.773 ± 0.049
2.845GlyPhe: 2.845 ± 0.035
8.087GlyGly: 8.087 ± 0.087
1.514GlyHis: 1.514 ± 0.029
3.769GlyIle: 3.769 ± 0.037
4.167GlyLys: 4.167 ± 0.043
5.982GlyLeu: 5.982 ± 0.046
1.777GlyMet: 1.777 ± 0.024
2.78GlyAsn: 2.78 ± 0.033
3.225GlyPro: 3.225 ± 0.045
2.223GlyGln: 2.223 ± 0.029
4.838GlyArg: 4.838 ± 0.045
6.201GlySer: 6.201 ± 0.066
3.951GlyThr: 3.951 ± 0.039
5.116GlyVal: 5.116 ± 0.047
1.171GlyTrp: 1.171 ± 0.021
2.169GlyTyr: 2.169 ± 0.028
0.001GlyXaa: 0.001 ± 0.0
His
1.547HisAla: 1.547 ± 0.025
0.3HisCys: 0.3 ± 0.011
1.07HisAsp: 1.07 ± 0.019
1.219HisGlu: 1.219 ± 0.021
0.877HisPhe: 0.877 ± 0.018
1.59HisGly: 1.59 ± 0.025
0.971HisHis: 0.971 ± 0.026
1.167HisIle: 1.167 ± 0.019
0.967HisLys: 0.967 ± 0.017
2.21HisLeu: 2.21 ± 0.025
0.463HisMet: 0.463 ± 0.011
0.806HisAsn: 0.806 ± 0.016
1.815HisPro: 1.815 ± 0.031
0.984HisGln: 0.984 ± 0.021
1.57HisArg: 1.57 ± 0.031
1.952HisSer: 1.952 ± 0.032
1.284HisThr: 1.284 ± 0.024
1.275HisVal: 1.275 ± 0.021
0.275HisTrp: 0.275 ± 0.008
0.671HisTyr: 0.671 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
3.987IleAla: 3.987 ± 0.039
0.733IleCys: 0.733 ± 0.015
2.653IleAsp: 2.653 ± 0.035
3.016IleGlu: 3.016 ± 0.038
1.957IlePhe: 1.957 ± 0.029
3.256IleGly: 3.256 ± 0.034
1.21IleHis: 1.21 ± 0.021
2.51IleIle: 2.51 ± 0.035
2.376IleLys: 2.376 ± 0.027
4.67IleLeu: 4.67 ± 0.049
0.99IleMet: 0.99 ± 0.017
1.769IleAsn: 1.769 ± 0.026
3.249IlePro: 3.249 ± 0.032
1.697IleGln: 1.697 ± 0.027
2.934IleArg: 2.934 ± 0.037
4.164IleSer: 4.164 ± 0.036
2.994IleThr: 2.994 ± 0.031
3.209IleVal: 3.209 ± 0.04
0.656IleTrp: 0.656 ± 0.014
1.402IleTyr: 1.402 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.208LysAla: 4.208 ± 0.049
0.579LysCys: 0.579 ± 0.013
2.756LysAsp: 2.756 ± 0.03
3.978LysGlu: 3.978 ± 0.051
1.651LysPhe: 1.651 ± 0.023
3.567LysGly: 3.567 ± 0.036
1.162LysHis: 1.162 ± 0.021
2.588LysIle: 2.588 ± 0.032
3.998LysLys: 3.998 ± 0.054
4.355LysLeu: 4.355 ± 0.045
1.128LysMet: 1.128 ± 0.017
1.936LysAsn: 1.936 ± 0.026
2.889LysPro: 2.889 ± 0.044
1.759LysGln: 1.759 ± 0.025
3.943LysArg: 3.943 ± 0.045
3.744LysSer: 3.744 ± 0.042
2.94LysThr: 2.94 ± 0.032
3.185LysVal: 3.185 ± 0.037
0.663LysTrp: 0.663 ± 0.015
1.464LysTyr: 1.464 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
7.25LeuAla: 7.25 ± 0.046
1.18LeuCys: 1.18 ± 0.021
4.774LeuAsp: 4.774 ± 0.051
5.873LeuGlu: 5.873 ± 0.063
3.361LeuPhe: 3.361 ± 0.041
6.162LeuGly: 6.162 ± 0.062
2.166LeuHis: 2.166 ± 0.03
3.876LeuIle: 3.876 ± 0.044
4.664LeuLys: 4.664 ± 0.051
8.628LeuLeu: 8.628 ± 0.081
1.761LeuMet: 1.761 ± 0.025
3.012LeuAsn: 3.012 ± 0.032
5.582LeuPro: 5.582 ± 0.049
3.429LeuGln: 3.429 ± 0.039
5.817LeuArg: 5.817 ± 0.048
7.376LeuSer: 7.376 ± 0.064
4.808LeuThr: 4.808 ± 0.047
5.447LeuVal: 5.447 ± 0.051
1.107LeuTrp: 1.107 ± 0.025
2.278LeuTyr: 2.278 ± 0.028
0.003LeuXaa: 0.003 ± 0.001
Met
1.949MetAla: 1.949 ± 0.026
0.259MetCys: 0.259 ± 0.01
1.241MetAsp: 1.241 ± 0.02
1.493MetGlu: 1.493 ± 0.022
0.749MetPhe: 0.749 ± 0.016
1.642MetGly: 1.642 ± 0.021
0.457MetHis: 0.457 ± 0.015
1.027MetIle: 1.027 ± 0.017
1.106MetLys: 1.106 ± 0.019
1.777MetLeu: 1.777 ± 0.023
0.55MetMet: 0.55 ± 0.016
0.788MetAsn: 0.788 ± 0.017
1.21MetPro: 1.21 ± 0.019
0.724MetGln: 0.724 ± 0.016
1.271MetArg: 1.271 ± 0.02
1.825MetSer: 1.825 ± 0.026
1.172MetThr: 1.172 ± 0.017
1.372MetVal: 1.372 ± 0.021
0.252MetTrp: 0.252 ± 0.01
0.508MetTyr: 0.508 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.73AsnAla: 2.73 ± 0.027
0.451AsnCys: 0.451 ± 0.011
1.709AsnAsp: 1.709 ± 0.019
1.946AsnGlu: 1.946 ± 0.025
1.378AsnPhe: 1.378 ± 0.025
2.906AsnGly: 2.906 ± 0.037
0.861AsnHis: 0.861 ± 0.017
1.982AsnIle: 1.982 ± 0.026
1.65AsnLys: 1.65 ± 0.024
3.277AsnLeu: 3.277 ± 0.037
0.777AsnMet: 0.777 ± 0.016
1.537AsnAsn: 1.537 ± 0.028
2.577AsnPro: 2.577 ± 0.036
1.169AsnGln: 1.169 ± 0.021
2.075AsnArg: 2.075 ± 0.028
2.994AsnSer: 2.994 ± 0.036
2.171AsnThr: 2.171 ± 0.03
2.213AsnVal: 2.213 ± 0.027
0.494AsnTrp: 0.494 ± 0.013
1.027AsnTyr: 1.027 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
4.928ProAla: 4.928 ± 0.063
0.507ProCys: 0.507 ± 0.015
2.825ProAsp: 2.825 ± 0.034
3.78ProGlu: 3.78 ± 0.037
2.024ProPhe: 2.024 ± 0.029
4.146ProGly: 4.146 ± 0.045
1.486ProHis: 1.486 ± 0.021
2.725ProIle: 2.725 ± 0.032
2.91ProLys: 2.91 ± 0.035
4.927ProLeu: 4.927 ± 0.043
1.035ProMet: 1.035 ± 0.021
2.226ProAsn: 2.226 ± 0.028
6.933ProPro: 6.933 ± 0.115
2.449ProGln: 2.449 ± 0.041
3.518ProArg: 3.518 ± 0.044
6.122ProSer: 6.122 ± 0.071
4.477ProThr: 4.477 ± 0.056
3.597ProVal: 3.597 ± 0.04
0.581ProTrp: 0.581 ± 0.014
1.45ProTyr: 1.45 ± 0.025
0.002ProXaa: 0.002 ± 0.001
Gln
2.765GlnAla: 2.765 ± 0.033
0.37GlnCys: 0.37 ± 0.01
1.666GlnAsp: 1.666 ± 0.024
2.245GlnGlu: 2.245 ± 0.028
1.181GlnPhe: 1.181 ± 0.023
2.153GlnGly: 2.153 ± 0.032
0.955GlnHis: 0.955 ± 0.021
1.697GlnIle: 1.697 ± 0.026
1.975GlnLys: 1.975 ± 0.029
3.044GlnLeu: 3.044 ± 0.037
0.784GlnMet: 0.784 ± 0.017
1.437GlnAsn: 1.437 ± 0.024
2.211GlnPro: 2.211 ± 0.04
2.432GlnGln: 2.432 ± 0.07
2.39GlnArg: 2.39 ± 0.03
2.677GlnSer: 2.677 ± 0.03
2.058GlnThr: 2.058 ± 0.031
1.961GlnVal: 1.961 ± 0.029
0.42GlnTrp: 0.42 ± 0.013
0.97GlnTyr: 0.97 ± 0.02
0.001GlnXaa: 0.001 ± 0.001
Arg
4.765ArgAla: 4.765 ± 0.043
0.742ArgCys: 0.742 ± 0.018
3.252ArgAsp: 3.252 ± 0.037
4.535ArgGlu: 4.535 ± 0.046
2.107ArgPhe: 2.107 ± 0.025
4.546ArgGly: 4.546 ± 0.045
1.486ArgHis: 1.486 ± 0.022
3.146ArgIle: 3.146 ± 0.037
4.062ArgLys: 4.062 ± 0.042
5.335ArgLeu: 5.335 ± 0.05
1.492ArgMet: 1.492 ± 0.022
2.389ArgAsn: 2.389 ± 0.03
3.327ArgPro: 3.327 ± 0.038
2.322ArgGln: 2.322 ± 0.033
5.522ArgArg: 5.522 ± 0.065
4.932ArgSer: 4.932 ± 0.049
3.27ArgThr: 3.27 ± 0.036
3.79ArgVal: 3.79 ± 0.041
0.864ArgTrp: 0.864 ± 0.019
1.708ArgTyr: 1.708 ± 0.024
0.001ArgXaa: 0.001 ± 0.0
Ser
6.434SerAla: 6.434 ± 0.052
0.872SerCys: 0.872 ± 0.019
3.822SerAsp: 3.822 ± 0.041
4.251SerGlu: 4.251 ± 0.043
2.935SerPhe: 2.935 ± 0.028
6.226SerGly: 6.226 ± 0.061
1.904SerHis: 1.904 ± 0.029
3.982SerIle: 3.982 ± 0.042
3.732SerLys: 3.732 ± 0.038
7.344SerLeu: 7.344 ± 0.056
1.688SerMet: 1.688 ± 0.022
2.893SerAsn: 2.893 ± 0.035
6.288SerPro: 6.288 ± 0.082
2.845SerGln: 2.845 ± 0.038
5.134SerArg: 5.134 ± 0.054
9.112SerSer: 9.112 ± 0.1
5.633SerThr: 5.633 ± 0.058
4.554SerVal: 4.554 ± 0.044
0.956SerTrp: 0.956 ± 0.018
2.021SerTyr: 2.021 ± 0.028
0.001SerXaa: 0.001 ± 0.001
Thr
4.836ThrAla: 4.836 ± 0.044
0.701ThrCys: 0.701 ± 0.017
2.524ThrAsp: 2.524 ± 0.027
3.025ThrGlu: 3.025 ± 0.036
2.156ThrPhe: 2.156 ± 0.03
4.292ThrGly: 4.292 ± 0.049
1.335ThrHis: 1.335 ± 0.02
3.118ThrIle: 3.118 ± 0.032
2.686ThrLys: 2.686 ± 0.031
5.328ThrLeu: 5.328 ± 0.052
1.07ThrMet: 1.07 ± 0.019
2.035ThrAsn: 2.035 ± 0.024
4.809ThrPro: 4.809 ± 0.052
1.878ThrGln: 1.878 ± 0.027
3.267ThrArg: 3.267 ± 0.036
5.313ThrSer: 5.313 ± 0.058
4.423ThrThr: 4.423 ± 0.057
3.502ThrVal: 3.502 ± 0.037
0.696ThrTrp: 0.696 ± 0.014
1.518ThrTyr: 1.518 ± 0.024
0.001ThrXaa: 0.001 ± 0.001
Val
4.913ValAla: 4.913 ± 0.042
0.858ValCys: 0.858 ± 0.016
3.563ValAsp: 3.563 ± 0.041
4.396ValGlu: 4.396 ± 0.044
2.431ValPhe: 2.431 ± 0.034
4.644ValGly: 4.644 ± 0.041
1.363ValHis: 1.363 ± 0.022
3.068ValIle: 3.068 ± 0.035
3.228ValLys: 3.228 ± 0.036
5.726ValLeu: 5.726 ± 0.054
1.398ValMet: 1.398 ± 0.02
2.147ValAsn: 2.147 ± 0.028
3.469ValPro: 3.469 ± 0.043
2.039ValGln: 2.039 ± 0.026
3.728ValArg: 3.728 ± 0.037
4.608ValSer: 4.608 ± 0.041
3.405ValThr: 3.405 ± 0.039
4.597ValVal: 4.597 ± 0.045
0.842ValTrp: 0.842 ± 0.018
1.713ValTyr: 1.713 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
0.945TrpAla: 0.945 ± 0.018
0.186TrpCys: 0.186 ± 0.007
0.803TrpAsp: 0.803 ± 0.021
0.874TrpGlu: 0.874 ± 0.018
0.45TrpPhe: 0.45 ± 0.012
1.004TrpGly: 1.004 ± 0.021
0.272TrpHis: 0.272 ± 0.009
0.663TrpIle: 0.663 ± 0.015
0.799TrpLys: 0.799 ± 0.017
1.161TrpLeu: 1.161 ± 0.02
0.331TrpMet: 0.331 ± 0.01
0.531TrpAsn: 0.531 ± 0.013
0.466TrpPro: 0.466 ± 0.013
0.409TrpGln: 0.409 ± 0.01
0.921TrpArg: 0.921 ± 0.018
0.905TrpSer: 0.905 ± 0.019
0.727TrpThr: 0.727 ± 0.017
0.861TrpVal: 0.861 ± 0.018
0.265TrpTrp: 0.265 ± 0.01
0.363TrpTyr: 0.363 ± 0.011
0.001TrpXaa: 0.001 ± 0.0
Tyr
1.898TyrAla: 1.898 ± 0.024
0.437TyrCys: 0.437 ± 0.014
1.535TyrAsp: 1.535 ± 0.025
1.549TyrGlu: 1.549 ± 0.023
1.181TyrPhe: 1.181 ± 0.021
1.911TyrGly: 1.911 ± 0.025
0.746TyrHis: 0.746 ± 0.015
1.383TyrIle: 1.383 ± 0.021
1.216TyrLys: 1.216 ± 0.039
2.597TyrLeu: 2.597 ± 0.031
0.551TyrMet: 0.551 ± 0.014
1.078TyrAsn: 1.078 ± 0.017
1.515TyrPro: 1.515 ± 0.024
0.994TyrGln: 0.994 ± 0.019
1.685TyrArg: 1.685 ± 0.026
2.124TyrSer: 2.124 ± 0.03
1.576TyrThr: 1.576 ± 0.027
1.553TyrVal: 1.553 ± 0.023
0.383TyrTrp: 0.383 ± 0.013
0.916TyrTyr: 0.916 ± 0.019
0.001TyrXaa: 0.001 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.0
0.001XaaGlu: 0.001 ± 0.001
0.001XaaPhe: 0.001 ± 0.0
0.002XaaGly: 0.002 ± 0.001
0.001XaaHis: 0.001 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.002XaaPro: 0.002 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.002XaaArg: 0.002 ± 0.001
0.002XaaSer: 0.002 ± 0.001
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.001XaaTrp: 0.001 ± 0.001
0.001XaaTyr: 0.001 ± 0.0
4.15XaaXaa: 4.15 ± 2.488
Statistics based on 7494 proteins (3279832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski