Pararhodobacter oceanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pararhodobacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T8HVW2|A0A2T8HVW2_9RHOB DsbE family thiol:disulfide interchange protein OS=Pararhodobacter oceanensis OX=2172121 GN=DDE20_05495 PE=3 SV=1
MM1 pKa = 7.48TKK3 pKa = 10.41FISGRR8 pKa = 11.84FKK10 pKa = 11.06GVGGGVAAVAALSLPVLPITAAAQSEE36 pKa = 4.6GAAGGFAIEE45 pKa = 4.71VGPSIDD51 pKa = 3.34PYY53 pKa = 11.03LPVRR57 pKa = 11.84VGARR61 pKa = 11.84VFGEE65 pKa = 3.86ASEE68 pKa = 4.22PASGFVRR75 pKa = 11.84GCQGFVLPEE84 pKa = 3.55SAGAVFEE91 pKa = 4.48VTEE94 pKa = 4.37EE95 pKa = 3.99MATLAFTGAAEE106 pKa = 4.45GLASMVLGTPDD117 pKa = 5.11GLFQCALADD126 pKa = 3.78EE127 pKa = 5.15RR128 pKa = 11.84GLAVVNMAGVAPGRR142 pKa = 11.84YY143 pKa = 9.08SVWLGAAEE151 pKa = 4.45GAQIDD156 pKa = 3.88ARR158 pKa = 11.84LFASDD163 pKa = 3.9SVISAIEE170 pKa = 3.83IFGLDD175 pKa = 3.74MAHH178 pKa = 6.35MGEE181 pKa = 4.33PRR183 pKa = 11.84VGRR186 pKa = 11.84HH187 pKa = 4.95VFSASLEE194 pKa = 4.21SGRR197 pKa = 11.84QEE199 pKa = 4.07LASGAIVSAVSEE211 pKa = 4.32MRR213 pKa = 11.84PLSPDD218 pKa = 3.11YY219 pKa = 11.29CPGYY223 pKa = 9.59TNFDD227 pKa = 3.36AADD230 pKa = 3.45AVLTLDD236 pKa = 4.23AEE238 pKa = 4.35IDD240 pKa = 3.76RR241 pKa = 11.84FSVFATSQRR250 pKa = 11.84DD251 pKa = 3.18LTMAVVQPDD260 pKa = 3.78GAVICNDD267 pKa = 3.86DD268 pKa = 4.13AFEE271 pKa = 4.03LHH273 pKa = 6.95PGVVIDD279 pKa = 4.37RR280 pKa = 11.84AQAGDD285 pKa = 3.33YY286 pKa = 10.43HH287 pKa = 7.69VFVGAYY293 pKa = 8.98SQGGTASFDD302 pKa = 3.6LFASEE307 pKa = 4.87GGPAFSNAILDD318 pKa = 3.91LDD320 pKa = 3.95AAPRR324 pKa = 11.84AGSALFDD331 pKa = 3.66MDD333 pKa = 5.1AAGQGQLLAAGQVSAVDD350 pKa = 3.79PMASLATGNYY360 pKa = 8.77CPGYY364 pKa = 8.84TDD366 pKa = 3.7ISAPDD371 pKa = 3.71FVMTMDD377 pKa = 3.56QAQPMISLYY386 pKa = 10.82ARR388 pKa = 11.84SEE390 pKa = 4.13TDD392 pKa = 2.82LVLAVRR398 pKa = 11.84APDD401 pKa = 3.98GTWLCNDD408 pKa = 4.9DD409 pKa = 5.21NYY411 pKa = 11.12QLNPGVTLQEE421 pKa = 4.16AQAGDD426 pKa = 3.39YY427 pKa = 10.54HH428 pKa = 7.55VFVGSYY434 pKa = 8.21HH435 pKa = 5.71TGAMGGYY442 pKa = 9.46NLYY445 pKa = 11.19ASMGAPNWQEE455 pKa = 3.9TQGGGGSGLGVLDD468 pKa = 3.75ITAEE472 pKa = 3.99PAIGRR477 pKa = 11.84IGFGPDD483 pKa = 2.99TQIDD487 pKa = 3.54PRR489 pKa = 11.84IIFDD493 pKa = 3.67IEE495 pKa = 3.94ASQTEE500 pKa = 4.47AFGMGPGCAGFITPEE515 pKa = 4.09RR516 pKa = 11.84PDD518 pKa = 3.46LVINAEE524 pKa = 4.09AGLPQLMVYY533 pKa = 8.39MASEE537 pKa = 4.22ADD539 pKa = 3.43GTLVISGPDD548 pKa = 3.45GQIYY552 pKa = 10.66CNDD555 pKa = 4.11DD556 pKa = 3.66FEE558 pKa = 4.53QLNPGVMIPNAPAGDD573 pKa = 3.66YY574 pKa = 10.99SVFAGTYY581 pKa = 9.48SGTGGLATLGVTIAAPQWVMDD602 pKa = 4.34RR603 pKa = 11.84EE604 pKa = 4.4HH605 pKa = 6.82

Molecular weight: 62.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T8HVS9|A0A2T8HVS9_9RHOB DNA-directed RNA polymerase subunit beta' OS=Pararhodobacter oceanensis OX=2172121 GN=rpoC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3661

0

3661

1181261

29

2887

322.7

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.982 ± 0.058

0.881 ± 0.012

5.694 ± 0.031

5.848 ± 0.035

3.654 ± 0.027

8.666 ± 0.036

2.146 ± 0.017

5.208 ± 0.025

2.622 ± 0.032

10.307 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.018

2.511 ± 0.022

5.223 ± 0.029

3.382 ± 0.023

6.873 ± 0.035

5.18 ± 0.028

5.348 ± 0.035

7.102 ± 0.037

1.386 ± 0.018

2.161 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski