Clostridium sp. CAG:149

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5MKX5|R5MKX5_9CLOT Endoribonuclease YbeY OS=Clostridium sp. CAG:149 OX=1262776 GN=ybeY PE=3 SV=1
MM1 pKa = 6.99FRR3 pKa = 11.84KK4 pKa = 9.66IACLMMAAVMIFSLSACGKK23 pKa = 9.85ARR25 pKa = 11.84PEE27 pKa = 4.25DD28 pKa = 3.92GEE30 pKa = 4.02ISVSIEE36 pKa = 3.91EE37 pKa = 4.54TPGGQVPDD45 pKa = 4.18GQEE48 pKa = 3.68KK49 pKa = 10.39DD50 pKa = 3.49AQEE53 pKa = 4.8PGTSLDD59 pKa = 3.54EE60 pKa = 4.26TEE62 pKa = 5.15AAQQEE67 pKa = 4.45QTASGTGNTLVVYY80 pKa = 7.33FSWSGNTEE88 pKa = 3.57EE89 pKa = 4.25MASYY93 pKa = 10.26LAEE96 pKa = 4.04QAGGEE101 pKa = 4.08LLEE104 pKa = 4.84IEE106 pKa = 4.45PAVPYY111 pKa = 10.53PEE113 pKa = 5.97DD114 pKa = 3.77YY115 pKa = 11.02SEE117 pKa = 5.18CGDD120 pKa = 3.53VAKK123 pKa = 10.82NEE125 pKa = 4.01RR126 pKa = 11.84DD127 pKa = 3.35EE128 pKa = 4.66DD129 pKa = 3.63EE130 pKa = 4.87RR131 pKa = 11.84PAIEE135 pKa = 4.61NLPEE139 pKa = 4.24SLEE142 pKa = 4.09QYY144 pKa = 9.49DD145 pKa = 4.62TILVGYY151 pKa = 7.0PIWWHH156 pKa = 5.05TAPMIIGTFLEE167 pKa = 4.87SYY169 pKa = 10.66DD170 pKa = 3.73LTGTDD175 pKa = 4.35IYY177 pKa = 10.61PFSQSASMDD186 pKa = 3.37TEE188 pKa = 3.96QFEE191 pKa = 4.27NSMDD195 pKa = 3.87FVRR198 pKa = 11.84EE199 pKa = 3.93SAGNANVHH207 pKa = 6.31EE208 pKa = 4.63GLFAEE213 pKa = 5.09PSDD216 pKa = 3.65TEE218 pKa = 5.11AIDD221 pKa = 4.77RR222 pKa = 11.84YY223 pKa = 9.25LTEE226 pKa = 4.69NGLIQQ231 pKa = 4.27

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5LYZ7|R5LYZ7_9CLOT ABC-type Fe3+ transport system periplasmic component OS=Clostridium sp. CAG:149 OX=1262776 GN=BN500_01278 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.57GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2596

0

2596

801876

29

2665

308.9

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.201 ± 0.052

1.511 ± 0.021

5.213 ± 0.044

8.281 ± 0.068

4.104 ± 0.032

7.924 ± 0.048

1.638 ± 0.022

6.58 ± 0.047

5.888 ± 0.047

9.286 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.093 ± 0.024

3.592 ± 0.028

3.559 ± 0.026

3.128 ± 0.027

5.615 ± 0.049

5.934 ± 0.044

5.053 ± 0.038

6.778 ± 0.04

0.922 ± 0.018

3.692 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski