Candidatus Tokpelaia hoelldoblerii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9JSK4|A0A1U9JSK4_9RHIZ 6-carboxy-5 6 7 8-tetrahydropterin synthase OS=Candidatus Tokpelaia hoelldoblerii OX=1902579 GN=queD PE=3 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.78VVTDD7 pKa = 2.96NCIRR11 pKa = 11.84CKK13 pKa = 10.23YY14 pKa = 7.51TDD16 pKa = 3.93CVEE19 pKa = 4.15VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.21NMLVIHH36 pKa = 7.24PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.44PEE49 pKa = 5.05CPAEE53 pKa = 4.42AIKK56 pKa = 10.65PDD58 pKa = 4.05TEE60 pKa = 4.72PGLDD64 pKa = 2.96QWLVLNAEE72 pKa = 4.8YY73 pKa = 10.26AAKK76 pKa = 9.48WPNITIKK83 pKa = 10.19KK84 pKa = 9.47DD85 pKa = 3.57QMPEE89 pKa = 3.57AAEE92 pKa = 3.95MDD94 pKa = 3.84GVPDD98 pKa = 3.19KK99 pKa = 10.99RR100 pKa = 11.84QYY102 pKa = 10.82FSEE105 pKa = 4.54KK106 pKa = 9.76PGSGDD111 pKa = 3.0

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9JWG6|A0A1U9JWG6_9RHIZ DUF2293 domain-containing protein OS=Candidatus Tokpelaia hoelldoblerii OX=1902579 GN=BHV28_14900 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84GVRR5 pKa = 11.84RR6 pKa = 11.84IITKK10 pKa = 9.83AAAGGVQLMGMLLFPPGCPGCGRR33 pKa = 11.84AIAAIGTICSDD44 pKa = 3.51CWLEE48 pKa = 5.08LPFLTPPFCPVMGIPFHH65 pKa = 7.25SDD67 pKa = 2.87YY68 pKa = 11.63GDD70 pKa = 4.76GILSGEE76 pKa = 4.41ALASPPPFDD85 pKa = 3.32HH86 pKa = 6.75ARR88 pKa = 11.84AVVAHH93 pKa = 6.77EE94 pKa = 3.87GLARR98 pKa = 11.84NLVTRR103 pKa = 11.84LKK105 pKa = 10.71YY106 pKa = 10.19GARR109 pKa = 11.84TDD111 pKa = 3.85LAPWMATWMVRR122 pKa = 11.84AGYY125 pKa = 10.14DD126 pKa = 3.47LVSACDD132 pKa = 3.67MIVPVPLHH140 pKa = 6.53RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84FWKK146 pKa = 10.19RR147 pKa = 11.84GYY149 pKa = 9.39NQSAEE154 pKa = 4.21LARR157 pKa = 11.84AIARR161 pKa = 11.84ICGKK165 pKa = 8.96PFCPQLLARR174 pKa = 11.84RR175 pKa = 11.84KK176 pKa = 6.41PTRR179 pKa = 11.84PQVGLSAKK187 pKa = 9.79RR188 pKa = 11.84RR189 pKa = 11.84ADD191 pKa = 3.25NVRR194 pKa = 11.84GVFAVPPRR202 pKa = 11.84GAAALKK208 pKa = 9.78HH209 pKa = 4.82KK210 pKa = 10.39HH211 pKa = 5.69IVLVDD216 pKa = 3.45DD217 pKa = 4.81VYY219 pKa = 9.55TTGATVKK226 pKa = 10.31AAARR230 pKa = 11.84ILKK233 pKa = 9.83RR234 pKa = 11.84AGAARR239 pKa = 11.84VDD241 pKa = 3.51VLTFSRR247 pKa = 11.84VVIRR251 pKa = 11.84YY252 pKa = 7.25EE253 pKa = 3.67

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1683

0

1683

556338

31

6177

330.6

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.415 ± 0.096

0.861 ± 0.021

5.461 ± 0.084

5.727 ± 0.064

3.986 ± 0.05

8.195 ± 0.089

2.159 ± 0.032

5.916 ± 0.05

4.347 ± 0.051

9.924 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.034

3.253 ± 0.058

4.469 ± 0.042

3.695 ± 0.038

6.446 ± 0.066

5.259 ± 0.046

5.31 ± 0.054

7.216 ± 0.051

1.164 ± 0.026

2.542 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski