Paracoccus sp. J56

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus; unclassified Paracoccus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3752 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7IFA6|A0A1X7IFA6_9RHOB Mannose-binding protein /fructose-binding protein /ribose-binding protein OS=Paracoccus sp. J56 OX=935850 GN=SAMN02746000_00664 PE=4 SV=1
MM1 pKa = 7.18ATINGTDD8 pKa = 3.63GADD11 pKa = 3.56TLTGTPDD18 pKa = 3.42ADD20 pKa = 4.44VINGLAGNDD29 pKa = 3.86VITGGSGNDD38 pKa = 3.54TLRR41 pKa = 11.84GGSGDD46 pKa = 3.64DD47 pKa = 3.25TFLIGGTSPGTDD59 pKa = 2.66IFDD62 pKa = 4.29GGDD65 pKa = 3.23GADD68 pKa = 3.99RR69 pKa = 11.84IRR71 pKa = 11.84LNGNLTVSNLQLTSANVISTEE92 pKa = 3.92TLDD95 pKa = 3.57FSIYY99 pKa = 10.18SIQGTGANDD108 pKa = 3.43TFNISGITYY117 pKa = 8.34TSSYY121 pKa = 11.14DD122 pKa = 2.87WFYY125 pKa = 11.48LYY127 pKa = 10.68DD128 pKa = 4.24GNDD131 pKa = 3.15SFVGYY136 pKa = 10.02VGNDD140 pKa = 3.4SVDD143 pKa = 3.24GGAGNDD149 pKa = 3.93TLNGGAGNDD158 pKa = 3.54VLTGGGGNDD167 pKa = 3.61HH168 pKa = 7.36LIGGSGNDD176 pKa = 3.29TFQIGGTDD184 pKa = 2.8IGYY187 pKa = 9.82DD188 pKa = 3.2IYY190 pKa = 11.62NGGDD194 pKa = 3.07GDD196 pKa = 4.16DD197 pKa = 4.47RR198 pKa = 11.84IRR200 pKa = 11.84LTSNVTVSNLQLTSANVISTEE221 pKa = 3.92TLDD224 pKa = 3.57FSIYY228 pKa = 10.18SIQGTGANDD237 pKa = 3.43TFNISGITYY246 pKa = 8.34TSSYY250 pKa = 11.14DD251 pKa = 2.87WFYY254 pKa = 11.48LYY256 pKa = 10.68DD257 pKa = 4.24GNDD260 pKa = 3.15SFVGYY265 pKa = 10.02VGNDD269 pKa = 3.4SVDD272 pKa = 3.24GGAGNDD278 pKa = 3.93TLNGGAGNDD287 pKa = 3.54VLTGGGGNDD296 pKa = 3.61HH297 pKa = 7.36LIGGSGNDD305 pKa = 3.29TFQIGGTDD313 pKa = 2.8IGYY316 pKa = 9.82DD317 pKa = 3.2IYY319 pKa = 11.62NGGDD323 pKa = 3.07GDD325 pKa = 4.16DD326 pKa = 4.47RR327 pKa = 11.84IRR329 pKa = 11.84LTSNVTVSNLQLTSANVISTEE350 pKa = 3.92TLDD353 pKa = 3.57FSIYY357 pKa = 10.18SIQGTGANDD366 pKa = 3.43TFNISGITYY375 pKa = 7.83TSSYY379 pKa = 11.0DD380 pKa = 3.05RR381 pKa = 11.84FYY383 pKa = 10.88MHH385 pKa = 7.78DD386 pKa = 3.96GNDD389 pKa = 3.26SFIGYY394 pKa = 9.19SGNDD398 pKa = 3.42AVDD401 pKa = 3.79GGAGNDD407 pKa = 3.78TLRR410 pKa = 11.84GEE412 pKa = 4.57AGNDD416 pKa = 3.28QLIGGSGNDD425 pKa = 3.41VLIGGTGNDD434 pKa = 3.84TLDD437 pKa = 3.87GGTGSDD443 pKa = 3.0TALYY447 pKa = 10.57DD448 pKa = 3.65GATSGVRR455 pKa = 11.84VNLGSTSAQAIGGGQGTDD473 pKa = 2.79VLTSIEE479 pKa = 4.16HH480 pKa = 5.18VRR482 pKa = 11.84GSRR485 pKa = 11.84YY486 pKa = 9.75SDD488 pKa = 3.29SLTGSGASNVLNAGAGNDD506 pKa = 4.03TLSGGAGNDD515 pKa = 3.29RR516 pKa = 11.84LIGGDD521 pKa = 3.4GNDD524 pKa = 3.65YY525 pKa = 11.22LKK527 pKa = 11.18GGTGNDD533 pKa = 3.63TLEE536 pKa = 4.69GGAGTDD542 pKa = 3.16TALYY546 pKa = 10.47DD547 pKa = 3.69SATGGVKK554 pKa = 10.42VNLGSASAQAIGGGQGTDD572 pKa = 2.86VLTGIEE578 pKa = 4.08NVRR581 pKa = 11.84GSRR584 pKa = 11.84YY585 pKa = 9.73NDD587 pKa = 3.24SLTGSGGNNILNAGAGNDD605 pKa = 4.03TLSGGAGNDD614 pKa = 3.39RR615 pKa = 11.84LIGGAGNDD623 pKa = 3.78YY624 pKa = 11.27LNGGTGNDD632 pKa = 3.79TLDD635 pKa = 3.58GSTGSDD641 pKa = 2.72TVLYY645 pKa = 10.6DD646 pKa = 3.45GATSAVTVNLGSTKK660 pKa = 10.37AQAIGGGYY668 pKa = 8.17GTDD671 pKa = 3.02VLSSIEE677 pKa = 3.93NVRR680 pKa = 11.84GSRR683 pKa = 11.84YY684 pKa = 9.67SDD686 pKa = 3.32SLTGSGGNNVLNAGAGNDD704 pKa = 4.03TLSGGAGSDD713 pKa = 3.47RR714 pKa = 11.84LIGGSGNDD722 pKa = 3.62YY723 pKa = 11.24LNGGTGNDD731 pKa = 3.62TLEE734 pKa = 4.85GGTGTDD740 pKa = 2.94TVLYY744 pKa = 10.38DD745 pKa = 3.61GATSAVTINLGSTKK759 pKa = 10.27AQAIGGGYY767 pKa = 8.17GTDD770 pKa = 3.02VLSSIEE776 pKa = 3.93NVRR779 pKa = 11.84GSRR782 pKa = 11.84YY783 pKa = 9.67SDD785 pKa = 3.32SLTGSGGNNVLNAGAGNDD803 pKa = 4.03TLSGGAGNDD812 pKa = 3.32RR813 pKa = 11.84LIGGSGNDD821 pKa = 3.62YY822 pKa = 11.24LNGGTGNDD830 pKa = 3.77TLDD833 pKa = 3.73GGTGIDD839 pKa = 3.17TALYY843 pKa = 10.57DD844 pKa = 4.03GATSGIKK851 pKa = 10.38VNLGSTSAQAIGGGYY866 pKa = 8.08GTDD869 pKa = 3.01VLNSIEE875 pKa = 4.26NIRR878 pKa = 11.84GSRR881 pKa = 11.84YY882 pKa = 9.57SDD884 pKa = 3.3SLTGSGANNVLNAGAGNDD902 pKa = 3.99TVSGGAGNDD911 pKa = 3.36QLIGGTGNDD920 pKa = 3.47RR921 pKa = 11.84LLGGTGNDD929 pKa = 3.77TLTGGAGNDD938 pKa = 3.62VLSGGSGADD947 pKa = 2.85VFVFSVRR954 pKa = 11.84DD955 pKa = 3.48NADD958 pKa = 4.85RR959 pKa = 11.84ISDD962 pKa = 3.67WQNGSDD968 pKa = 4.15VIRR971 pKa = 11.84IVAGTYY977 pKa = 10.08DD978 pKa = 3.53GEE980 pKa = 4.78RR981 pKa = 11.84YY982 pKa = 10.4DD983 pKa = 5.18SFDD986 pKa = 4.77DD987 pKa = 3.97LTITQSGSNTVVSFGGTTVTLTGINSGLLDD1017 pKa = 3.81ASDD1020 pKa = 3.93FVFVV1024 pKa = 4.16

Molecular weight: 101.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7KEG8|A0A1X7KEG8_9RHOB Putative spermidine/putrescine transport system permease protein OS=Paracoccus sp. J56 OX=935850 GN=SAMN02746000_02353 PE=3 SV=1
MM1 pKa = 7.0TLIWMTAIAMVSAGMVALRR20 pKa = 11.84LRR22 pKa = 11.84APQPQAVAARR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 3.41

Molecular weight: 3.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3752

0

3752

1148692

11

2099

306.2

33.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.762 ± 0.059

0.862 ± 0.012

5.606 ± 0.03

5.845 ± 0.038

3.481 ± 0.026

8.888 ± 0.043

2.098 ± 0.019

5.267 ± 0.028

2.699 ± 0.029

10.421 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.02

2.408 ± 0.019

5.489 ± 0.03

3.295 ± 0.021

7.584 ± 0.042

4.954 ± 0.026

5.117 ± 0.026

7.047 ± 0.035

1.439 ± 0.015

2.041 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski