Marinobacter vinifirmus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4CRA7|A0A2T4CRA7_9ALTE Polymerase (Fragment) OS=Marinobacter vinifirmus OX=355591 GN=C9985_01025 PE=4 SV=1
MM1 pKa = 7.49TIKK4 pKa = 10.68KK5 pKa = 9.28NVLIASVATAGIAMAGAASAQDD27 pKa = 3.74MYY29 pKa = 11.44KK30 pKa = 10.43SGVGGLYY37 pKa = 10.58AGGNYY42 pKa = 9.16TFMNADD48 pKa = 3.26FDD50 pKa = 4.68GVGDD54 pKa = 4.22ADD56 pKa = 3.76VGTLSGKK63 pKa = 9.25VGVMATEE70 pKa = 4.07YY71 pKa = 10.64FGLEE75 pKa = 3.49ARR77 pKa = 11.84AGFGVDD83 pKa = 3.47DD84 pKa = 3.93DD85 pKa = 4.4TVRR88 pKa = 11.84GVDD91 pKa = 3.49VEE93 pKa = 4.24LDD95 pKa = 3.6NFFGGYY101 pKa = 8.27ATFNMVNEE109 pKa = 4.45SPITPYY115 pKa = 10.93GVLGFTRR122 pKa = 11.84IEE124 pKa = 3.94AQAGNVEE131 pKa = 4.9DD132 pKa = 5.17DD133 pKa = 5.24DD134 pKa = 6.03SDD136 pKa = 3.72FSYY139 pKa = 11.1GAGVNFAFAPNLSANLEE156 pKa = 4.0YY157 pKa = 10.04MRR159 pKa = 11.84YY160 pKa = 9.91YY161 pKa = 10.81DD162 pKa = 5.2DD163 pKa = 5.33NDD165 pKa = 3.49VTVDD169 pKa = 3.44GLGIGMQVNFF179 pKa = 4.47

Molecular weight: 18.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4CRF6|A0A2T4CRF6_9ALTE Tripartite tricarboxylate transporter TctB family protein OS=Marinobacter vinifirmus OX=355591 GN=C9985_00865 PE=4 SV=1
MM1 pKa = 7.5IMHH4 pKa = 7.85LFRR7 pKa = 11.84VSPDD11 pKa = 3.36DD12 pKa = 3.92DD13 pKa = 3.99ALRR16 pKa = 11.84GALLMTGAAILFAAMAVLIRR36 pKa = 11.84YY37 pKa = 6.59ITRR40 pKa = 11.84EE41 pKa = 3.73VHH43 pKa = 5.55PFEE46 pKa = 5.03AAFFRR51 pKa = 11.84NLFGLIPMIPWMLRR65 pKa = 11.84DD66 pKa = 3.56RR67 pKa = 11.84LAGLRR72 pKa = 11.84TLKK75 pKa = 10.77LKK77 pKa = 10.63LHH79 pKa = 7.21FIRR82 pKa = 11.84AVIGLAGMLCLFSALAIAPAAQVIAINFTVPILTTILAALILHH125 pKa = 5.3EE126 pKa = 4.4TVRR129 pKa = 11.84ARR131 pKa = 11.84RR132 pKa = 11.84WTAVALGFIGAMIIVRR148 pKa = 11.84PIGQTLEE155 pKa = 3.96TGAILALLATLFTAFAVTTVKK176 pKa = 9.92MLSRR180 pKa = 11.84SEE182 pKa = 4.06SANAIVTWMGMIMTPLSLIPALFFWQNPTWEE213 pKa = 4.15QLGVLLAIAVLATAGQQLFVRR234 pKa = 11.84ANRR237 pKa = 11.84TADD240 pKa = 3.11QSYY243 pKa = 10.79VMAFDD248 pKa = 4.27FLRR251 pKa = 11.84LPFVAALAFIMFGEE265 pKa = 4.71TVDD268 pKa = 3.87FWTWAGASLIIGSTLYY284 pKa = 9.74IARR287 pKa = 11.84RR288 pKa = 11.84EE289 pKa = 3.91AVLARR294 pKa = 11.84KK295 pKa = 8.29EE296 pKa = 3.81KK297 pKa = 10.14RR298 pKa = 11.84RR299 pKa = 11.84RR300 pKa = 11.84RR301 pKa = 11.84MPTTAAADD309 pKa = 3.69SQAIPVTKK317 pKa = 10.21AKK319 pKa = 9.81TINPPEE325 pKa = 4.25YY326 pKa = 10.15PEE328 pKa = 4.44GTPEE332 pKa = 4.29SNTGNKK338 pKa = 8.12TGRR341 pKa = 11.84IDD343 pKa = 3.13AA344 pKa = 4.83

Molecular weight: 37.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

263

0

263

78148

25

1690

297.1

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.298 ± 0.174

0.909 ± 0.049

5.908 ± 0.16

6.023 ± 0.173

4.137 ± 0.13

8.118 ± 0.168

2.07 ± 0.075

6.063 ± 0.125

3.751 ± 0.125

10.112 ± 0.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.077

3.291 ± 0.075

4.714 ± 0.1

3.404 ± 0.095

6.222 ± 0.138

5.675 ± 0.111

5.266 ± 0.084

7.449 ± 0.14

1.319 ± 0.068

2.523 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski