Ralstonia phage RSB2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Kelmasvirus; Ralstonia virus RSB2

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5RV05|E5RV05_9CAUD Putative ssDNA binding protein OS=Ralstonia phage RSB2 OX=913183 GN=ORF25 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.47FKK4 pKa = 10.99LDD6 pKa = 3.26GAAWLVTEE14 pKa = 4.68RR15 pKa = 11.84CKK17 pKa = 11.09APNIDD22 pKa = 4.68LAFVWAEE29 pKa = 3.97EE30 pKa = 4.23FCDD33 pKa = 3.68ANHH36 pKa = 6.35GFEE39 pKa = 3.92VDD41 pKa = 4.33YY42 pKa = 10.97IAEE45 pKa = 4.15LL46 pKa = 3.65

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5RV03|E5RV03_9CAUD Uncharacterized protein ORF23 OS=Ralstonia phage RSB2 OX=913183 GN=ORF23 PE=4 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84EE4 pKa = 3.64LRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84DD9 pKa = 3.32NGRR12 pKa = 11.84KK13 pKa = 9.12LVVVPCSRR21 pKa = 11.84TVDD24 pKa = 3.66CVRR27 pKa = 11.84NANGNRR33 pKa = 11.84KK34 pKa = 9.64AEE36 pKa = 4.21ATFEE40 pKa = 4.39PPFKK44 pKa = 10.41TRR46 pKa = 11.84AVKK49 pKa = 10.69LGTDD53 pKa = 3.88WYY55 pKa = 11.34LLTQPVYY62 pKa = 11.22AEE64 pKa = 4.16ALDD67 pKa = 3.89NKK69 pKa = 9.44RR70 pKa = 11.84TTLTEE75 pKa = 3.63YY76 pKa = 9.56TGRR79 pKa = 11.84FPRR82 pKa = 11.84VAALWINLKK91 pKa = 10.53EE92 pKa = 4.1KK93 pKa = 10.62FKK95 pKa = 11.09RR96 pKa = 3.66

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12182

33

1290

234.3

25.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.885 ± 0.504

0.952 ± 0.17

6.189 ± 0.214

6.255 ± 0.262

3.965 ± 0.177

8.324 ± 0.356

2.028 ± 0.237

4.909 ± 0.193

5.492 ± 0.316

7.88 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.14

3.727 ± 0.218

4.449 ± 0.196

4.425 ± 0.295

5.82 ± 0.223

4.95 ± 0.243

6.099 ± 0.393

7.298 ± 0.244

1.445 ± 0.149

2.848 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski