Flavilitoribacter nigricans DSM 23189 = NBRC 102662

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; Flavilitoribacter; Flavilitoribacter nigricans

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0N4K6|A0A2D0N4K6_9BACT ATP phosphoribosyltransferase OS=Flavilitoribacter nigricans DSM 23189 = NBRC 102662 OX=1122177 GN=hisG PE=3 SV=1
MM1 pKa = 7.64TILEE5 pKa = 4.53KK6 pKa = 11.08YY7 pKa = 9.75NYY9 pKa = 8.96PIKK12 pKa = 9.96TLFAALILVGGFSCTRR28 pKa = 11.84DD29 pKa = 3.47LDD31 pKa = 4.07EE32 pKa = 6.75LEE34 pKa = 4.96LATFPTTAEE43 pKa = 3.86VFIDD47 pKa = 4.31GFSSGLNYY55 pKa = 10.6AAFGGSKK62 pKa = 6.79TTAFNVVDD70 pKa = 3.95DD71 pKa = 4.42EE72 pKa = 5.16GYY74 pKa = 10.84NNSAVMRR81 pKa = 11.84FDD83 pKa = 5.1IPDD86 pKa = 3.88ADD88 pKa = 4.05DD89 pKa = 4.4PGGGFAAGIFFTDD102 pKa = 3.28VARR105 pKa = 11.84DD106 pKa = 3.41LSGYY110 pKa = 10.31NVLTFWAKK118 pKa = 10.2ASEE121 pKa = 4.34SVDD124 pKa = 3.2VDD126 pKa = 3.34EE127 pKa = 6.15LGFGFTFQSEE137 pKa = 4.9KK138 pKa = 10.09YY139 pKa = 9.08RR140 pKa = 11.84SAIKK144 pKa = 9.71EE145 pKa = 4.06IPVTTNWKK153 pKa = 9.93KK154 pKa = 10.8YY155 pKa = 8.79YY156 pKa = 10.11IPIADD161 pKa = 3.48ASKK164 pKa = 8.89LTEE167 pKa = 4.16EE168 pKa = 4.47NGMFYY173 pKa = 10.76YY174 pKa = 10.54VDD176 pKa = 3.99SPDD179 pKa = 4.06DD180 pKa = 4.02GEE182 pKa = 6.28GYY184 pKa = 8.29TFWIDD189 pKa = 3.32EE190 pKa = 4.44VKK192 pKa = 10.77FEE194 pKa = 4.48NLSTLARR201 pKa = 11.84PGVRR205 pKa = 11.84ILAGQDD211 pKa = 3.19QSITAEE217 pKa = 4.11TGNQLNIGEE226 pKa = 4.24ISYY229 pKa = 8.16TANLPTGIDD238 pKa = 3.33QSVDD242 pKa = 2.44ISAAYY247 pKa = 8.47LTFTSSNSGVATVDD261 pKa = 3.1EE262 pKa = 4.58TGKK265 pKa = 8.65VTILDD270 pKa = 3.84AGNTVITATLGDD282 pKa = 4.2LEE284 pKa = 4.55ATGSLTIEE292 pKa = 4.27SSGDD296 pKa = 3.35PLLPATAAPVPTVGQDD312 pKa = 3.37SVISLFSNAYY322 pKa = 10.24DD323 pKa = 4.22DD324 pKa = 5.17VPVDD328 pKa = 3.06TWNPFWQFSTAEE340 pKa = 4.0VSDD343 pKa = 3.82VKK345 pKa = 10.78IGEE348 pKa = 4.78DD349 pKa = 3.85DD350 pKa = 3.77LKK352 pKa = 11.04RR353 pKa = 11.84YY354 pKa = 10.35NNLNFVGILTEE365 pKa = 4.12SAPIDD370 pKa = 3.87ASEE373 pKa = 4.1MTHH376 pKa = 5.27FHH378 pKa = 6.31IDD380 pKa = 2.35IWTPDD385 pKa = 3.47PTAPPATFKK394 pKa = 11.21VLLVDD399 pKa = 5.83FGADD403 pKa = 3.35GNFDD407 pKa = 3.79GGDD410 pKa = 3.76DD411 pKa = 3.94SSHH414 pKa = 6.33EE415 pKa = 4.17LTFTSPTLTTGSWVSLDD432 pKa = 3.4IPLSDD437 pKa = 3.64FTGLANRR444 pKa = 11.84NHH446 pKa = 6.87IAQLVLSGDD455 pKa = 3.92LPNLFVDD462 pKa = 4.19NVYY465 pKa = 10.2FYY467 pKa = 10.7QAGVVPVAEE476 pKa = 4.36PAEE479 pKa = 4.29AAPEE483 pKa = 3.94PTAAASDD490 pKa = 4.0VLSVFSDD497 pKa = 3.86AYY499 pKa = 11.43SNIEE503 pKa = 3.96GTDD506 pKa = 3.68LNPNWGQATVVSQVDD521 pKa = 3.21IAGNNTLLYY530 pKa = 10.75SGLNYY535 pKa = 10.27QGIQLGSNQDD545 pKa = 3.23VSGMEE550 pKa = 4.37FLHH553 pKa = 7.25IDD555 pKa = 3.14YY556 pKa = 7.68WTANSSGLNTFLISRR571 pKa = 11.84GPVEE575 pKa = 3.77TAYY578 pKa = 10.65ALPVPTTGWASVDD591 pKa = 3.12IPLSDD596 pKa = 4.53FAPVDD601 pKa = 3.88LTDD604 pKa = 4.37VIQLKK609 pKa = 10.55FDD611 pKa = 3.94GNGNIYY617 pKa = 10.33LDD619 pKa = 3.79NIYY622 pKa = 10.24FYY624 pKa = 9.67KK625 pKa = 10.32TGGGGMEE632 pKa = 4.34PAVSAPQPTEE642 pKa = 3.52AASEE646 pKa = 4.44VIALFSDD653 pKa = 4.36AYY655 pKa = 10.75ADD657 pKa = 3.63VVVDD661 pKa = 3.37TWRR664 pKa = 11.84TDD666 pKa = 2.31WSAAMLEE673 pKa = 4.35DD674 pKa = 4.04VSVAGNAVKK683 pKa = 10.29KK684 pKa = 10.54YY685 pKa = 10.73SNLDD689 pKa = 3.27FVGIEE694 pKa = 4.24TVANTVDD701 pKa = 3.51ASGMTHH707 pKa = 6.17FRR709 pKa = 11.84MDD711 pKa = 2.66VWSPDD716 pKa = 3.14FTFFGIKK723 pKa = 10.18LVDD726 pKa = 4.28FGTDD730 pKa = 3.15GAFGGGDD737 pKa = 3.43DD738 pKa = 5.13VEE740 pKa = 4.36HH741 pKa = 6.55QINFEE746 pKa = 4.24APAQGQWISYY756 pKa = 8.29DD757 pKa = 3.22IPLSDD762 pKa = 4.22FAGLTTRR769 pKa = 11.84SNIAQYY775 pKa = 10.95ILVGQPTGTTTVYY788 pKa = 10.5VDD790 pKa = 3.17NVYY793 pKa = 10.43FYY795 pKa = 11.43NSEE798 pKa = 3.92GGNNATEE805 pKa = 4.62PGLAAPTPTEE815 pKa = 4.22DD816 pKa = 3.51ASDD819 pKa = 4.04VISLFSEE826 pKa = 5.09AYY828 pKa = 10.47SNVAVDD834 pKa = 3.19TWRR837 pKa = 11.84TDD839 pKa = 2.3WSAAMLEE846 pKa = 4.35DD847 pKa = 4.04VSVAGNAVKK856 pKa = 10.29KK857 pKa = 10.54YY858 pKa = 10.73SNLDD862 pKa = 3.27FVGIEE867 pKa = 4.19TVANPIDD874 pKa = 4.17ASGMTYY880 pKa = 9.57FHH882 pKa = 7.07IDD884 pKa = 2.69VWSSDD889 pKa = 3.44FTFFGIKK896 pKa = 10.18LVDD899 pKa = 4.74FGADD903 pKa = 3.24GAFGGGDD910 pKa = 3.43DD911 pKa = 5.13VEE913 pKa = 4.36HH914 pKa = 6.55QINFEE919 pKa = 4.24APAQGQWISYY929 pKa = 8.36DD930 pKa = 3.17IPLDD934 pKa = 3.9DD935 pKa = 5.23FVGLTTRR942 pKa = 11.84SNMAQYY948 pKa = 10.75ILVGQPIGATTVYY961 pKa = 10.07VDD963 pKa = 2.98NVYY966 pKa = 10.29FHH968 pKa = 7.41KK969 pKa = 10.95

Molecular weight: 104.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0NFV0|A0A2D0NFV0_9BACT Uncharacterized protein OS=Flavilitoribacter nigricans DSM 23189 = NBRC 102662 OX=1122177 GN=CRP01_06400 PE=4 SV=1
MM1 pKa = 7.52HH2 pKa = 7.31SGFGAEE8 pKa = 4.07VLLIARR14 pKa = 11.84MPKK17 pKa = 10.13PMLRR21 pKa = 11.84QCSCSSAWFRR31 pKa = 11.84LPKK34 pKa = 10.34AEE36 pKa = 4.39PRR38 pKa = 11.84GLLIRR43 pKa = 11.84WINIQAPYY51 pKa = 10.56ASEE54 pKa = 4.51LLGQATIRR62 pKa = 11.84KK63 pKa = 7.92NHH65 pKa = 6.05FSALQILPFLFF76 pKa = 4.38

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8179

0

8179

3270642

38

8758

399.9

44.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.554 ± 0.025

0.919 ± 0.022

6.168 ± 0.038

6.573 ± 0.026

4.715 ± 0.021

7.336 ± 0.029

1.919 ± 0.016

6.331 ± 0.021

4.659 ± 0.039

9.888 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.015

4.603 ± 0.022

4.499 ± 0.023

4.163 ± 0.022

5.267 ± 0.036

6.046 ± 0.025

5.706 ± 0.063

6.166 ± 0.03

1.451 ± 0.011

3.914 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski