Vibrio phage NF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J178|A0A6B9J178_9CAUD Putative exonuclease OS=Vibrio phage NF OX=2686202 PE=4 SV=1
MM1 pKa = 7.74SEE3 pKa = 4.02QLKK6 pKa = 10.55SDD8 pKa = 3.58LSIFIPYY15 pKa = 9.6CLLGISWAYY24 pKa = 10.0GVVSLFEE31 pKa = 3.89WAIEE35 pKa = 3.96LGVII39 pKa = 4.71

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J049|A0A6B9J049_9CAUD BIG2 domain-containing protein OS=Vibrio phage NF OX=2686202 PE=4 SV=1
MM1 pKa = 7.78SKK3 pKa = 10.27RR4 pKa = 11.84KK5 pKa = 9.43SNKK8 pKa = 8.7RR9 pKa = 11.84VKK11 pKa = 10.78QMICDD16 pKa = 3.81VLVSSSKK23 pKa = 10.26LSKK26 pKa = 10.26VRR28 pKa = 11.84YY29 pKa = 8.53GYY31 pKa = 11.0YY32 pKa = 9.34RR33 pKa = 11.84VKK35 pKa = 10.23FCNVRR40 pKa = 11.84GDD42 pKa = 3.94FVQSINYY49 pKa = 9.15GRR51 pKa = 11.84FHH53 pKa = 7.25FFNSGRR59 pKa = 11.84YY60 pKa = 8.35VSLTASEE67 pKa = 4.21LRR69 pKa = 11.84GG70 pKa = 3.48

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13027

39

1174

176.0

19.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.024 ± 0.511

1.428 ± 0.177

6.502 ± 0.289

7.001 ± 0.35

3.815 ± 0.158

7.569 ± 0.317

1.85 ± 0.187

6.586 ± 0.226

7.154 ± 0.48

7.131 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.22

5.205 ± 0.236

3.454 ± 0.234

4.13 ± 0.255

4.629 ± 0.206

6.87 ± 0.342

5.028 ± 0.347

6.563 ± 0.291

1.842 ± 0.158

3.531 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski