uncultured phage_MedDCM-OCT-S37-C6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Oinezvirus; Oinezvirus S37C6

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PGQ3|A0A6S4PGQ3_9CAUD Gp161 OS=uncultured phage_MedDCM-OCT-S37-C6 OX=2740804 PE=4 SV=1
MM1 pKa = 7.16PVSYY5 pKa = 9.18STHH8 pKa = 5.27TVASTATGSALQFAVSYY25 pKa = 9.17PYY27 pKa = 10.39ILRR30 pKa = 11.84SHH32 pKa = 5.96VKK34 pKa = 9.12VYY36 pKa = 10.21YY37 pKa = 10.78GRR39 pKa = 11.84DD40 pKa = 3.16ILAGTHH46 pKa = 5.77TSLLVDD52 pKa = 3.35GTDD55 pKa = 4.0YY56 pKa = 11.7NWTTDD61 pKa = 3.45TQITLSAAPSAQSTLTIIRR80 pKa = 11.84EE81 pKa = 4.45TPTSAQLVPWQDD93 pKa = 3.01GSNLIAEE100 pKa = 5.22DD101 pKa = 4.15LNKK104 pKa = 10.07SDD106 pKa = 4.05KK107 pKa = 10.51QNLYY111 pKa = 10.61AVQEE115 pKa = 4.37VQDD118 pKa = 4.09KK119 pKa = 10.57NQLASDD125 pKa = 4.26DD126 pKa = 3.74ATAASTAANAATTAANAATTAANNANTAATAATADD161 pKa = 3.4SAAAVTTANAASATANTANTNATAAQAAATAAQTSATNAATDD203 pKa = 3.32AAAAQTSATAAQTSATAAQTSATAAQTSATSAATDD238 pKa = 3.36AASAISTANAASTTATTALNNSRR261 pKa = 11.84QSDD264 pKa = 3.98GQGGFTTAISLANTANTTANSATSTANTASTNASSAVTTANAASATASAASAAVANAAFYY324 pKa = 11.29SPVAALANLPSSPANEE340 pKa = 4.25DD341 pKa = 3.09RR342 pKa = 11.84VEE344 pKa = 4.44VIDD347 pKa = 3.72STGVQSSSAVSGVPSGFTGSTNLTVRR373 pKa = 11.84LQYY376 pKa = 9.69STSSTKK382 pKa = 9.96WEE384 pKa = 3.81WQQYY388 pKa = 7.93FAADD392 pKa = 3.55PEE394 pKa = 4.2NRR396 pKa = 11.84YY397 pKa = 8.09ATSYY401 pKa = 11.05LPVIKK406 pKa = 10.55GDD408 pKa = 3.86GTSSGQVGKK417 pKa = 8.7ITLNCSNNNHH427 pKa = 5.77GVSIQSPAHH436 pKa = 5.76SAGATYY442 pKa = 10.27TLTLPVNTGSANQALTTNGQGVLSWADD469 pKa = 3.24AGSPTIDD476 pKa = 3.04GGNFNSGGSLVTTTQTIDD494 pKa = 3.09GGSFNN499 pKa = 4.79

Molecular weight: 49.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PM50|A0A6S4PM50_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S37-C6 OX=2740804 PE=4 SV=1
MM1 pKa = 7.63CFGGGGRR8 pKa = 11.84ATITKK13 pKa = 9.33PDD15 pKa = 3.39YY16 pKa = 10.52NAYY19 pKa = 10.28DD20 pKa = 3.62KK21 pKa = 11.43QFDD24 pKa = 3.96LQKK27 pKa = 10.85EE28 pKa = 4.51AIQNSMDD35 pKa = 3.48SNLRR39 pKa = 11.84LKK41 pKa = 9.92QQEE44 pKa = 4.22LQASLGDD51 pKa = 3.4KK52 pKa = 10.97ADD54 pKa = 3.26IQRR57 pKa = 11.84QLNEE61 pKa = 3.9QIRR64 pKa = 11.84IQAEE68 pKa = 4.04NTNAQAMRR76 pKa = 11.84LAQVIGPPPPEE87 pKa = 4.74KK88 pKa = 10.33SAQAPVIGSKK98 pKa = 10.05RR99 pKa = 11.84KK100 pKa = 9.41KK101 pKa = 9.98LGKK104 pKa = 9.12EE105 pKa = 3.42RR106 pKa = 11.84LRR108 pKa = 11.84IRR110 pKa = 11.84RR111 pKa = 11.84AQSPTSSASGAGLNITANRR130 pKa = 3.81

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12361

48

1231

274.7

30.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.075 ± 0.669

0.947 ± 0.206

5.752 ± 0.213

5.639 ± 0.484

3.236 ± 0.236

7.629 ± 0.377

1.634 ± 0.199

4.126 ± 0.173

4.668 ± 0.438

8.438 ± 0.373

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.29

4.652 ± 0.462

4.53 ± 0.29

5.129 ± 0.541

5.679 ± 0.444

6.626 ± 0.682

6.925 ± 0.65

6.448 ± 0.258

1.432 ± 0.211

2.92 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski