Streptococcus pneumoniae SP14-BS69

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus pneumoniae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5M946|A5M946_STREE Probable GTP-binding protein EngB OS=Streptococcus pneumoniae SP14-BS69 OX=406560 GN=engB PE=3 SV=1
MM1 pKa = 7.87EE2 pKa = 4.51EE3 pKa = 3.56TTYY6 pKa = 11.03DD7 pKa = 3.82IIAKK11 pKa = 9.74SLDD14 pKa = 4.0RR15 pKa = 11.84ISMEE19 pKa = 3.74LHH21 pKa = 5.91QADD24 pKa = 4.33EE25 pKa = 4.59NNDD28 pKa = 3.14FLEE31 pKa = 4.3YY32 pKa = 10.95GFYY35 pKa = 10.31QDD37 pKa = 3.87NN38 pKa = 3.58

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5MAD2|A5MAD2_STREE DNA polymerase IV OS=Streptococcus pneumoniae SP14-BS69 OX=406560 GN=dinB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.24QPSKK9 pKa = 9.82LRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.57HH16 pKa = 4.75GFRR19 pKa = 11.84NRR21 pKa = 11.84MSTKK25 pKa = 9.22NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2767

0

2767

614891

13

1883

222.2

25.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.056

0.643 ± 0.015

5.522 ± 0.046

7.279 ± 0.051

4.651 ± 0.046

6.474 ± 0.052

1.939 ± 0.022

7.233 ± 0.051

7.005 ± 0.047

10.181 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.021

4.425 ± 0.039

3.255 ± 0.027

4.087 ± 0.042

4.151 ± 0.038

6.219 ± 0.035

5.482 ± 0.038

6.926 ± 0.046

0.969 ± 0.019

3.818 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski