Mycolicibacterium agri (Mycobacterium agri)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A7N8R5|A0A2A7N8R5_MYCAG Hydrogenase maturation factor HypA OS=Mycolicibacterium agri OX=36811 GN=hypA PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84LPRR5 pKa = 11.84AVVAAAAAVLLAAAGCTTEE24 pKa = 4.05VTGTAVQDD32 pKa = 3.67LRR34 pKa = 11.84QPPLGLSEE42 pKa = 4.93DD43 pKa = 3.71GSGIVAGYY51 pKa = 9.56PDD53 pKa = 5.05APVQLEE59 pKa = 4.8VYY61 pKa = 9.42TEE63 pKa = 4.08PQCTHH68 pKa = 6.52CADD71 pKa = 4.38FQAEE75 pKa = 4.22FGDD78 pKa = 5.0DD79 pKa = 2.67IHH81 pKa = 8.79RR82 pKa = 11.84YY83 pKa = 9.21INLGQLAVTYY93 pKa = 10.41RR94 pKa = 11.84PLTFLDD100 pKa = 3.18EE101 pKa = 5.18GYY103 pKa = 9.39TDD105 pKa = 3.4GHH107 pKa = 5.54SARR110 pKa = 11.84VANAMFLAVGVPAEE124 pKa = 4.19AADD127 pKa = 3.98GDD129 pKa = 4.64FATGPEE135 pKa = 4.07FQRR138 pKa = 11.84FVQEE142 pKa = 3.68LWANQDD148 pKa = 3.3PGGPGPSDD156 pKa = 4.36ADD158 pKa = 3.46MADD161 pKa = 3.37MAKK164 pKa = 10.4AAGMPDD170 pKa = 3.71DD171 pKa = 3.95VAAQIGDD178 pKa = 3.9GEE180 pKa = 4.43VAPNVDD186 pKa = 3.06IAEE189 pKa = 4.23AAIFNYY195 pKa = 10.0GALIGVDD202 pKa = 4.66PITTGTPTVYY212 pKa = 10.75DD213 pKa = 3.9LDD215 pKa = 3.82MQEE218 pKa = 5.35KK219 pKa = 10.58INIQDD224 pKa = 4.67DD225 pKa = 3.77DD226 pKa = 4.08WLDD229 pKa = 3.62TLMSSAA235 pKa = 4.71

Molecular weight: 24.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A7N5W8|A0A2A7N5W8_MYCAG Cytoplasmic protein OS=Mycolicibacterium agri OX=36811 GN=CQY20_09775 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSNRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.47GRR42 pKa = 11.84RR43 pKa = 11.84ALSAA47 pKa = 3.69

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6452

0

6452

2059546

24

7553

319.2

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.766 ± 0.046

0.846 ± 0.008

6.304 ± 0.029

5.481 ± 0.028

3.102 ± 0.019

8.596 ± 0.029

2.188 ± 0.013

4.473 ± 0.02

2.386 ± 0.022

9.769 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.013

2.321 ± 0.014

5.786 ± 0.028

2.97 ± 0.016

7.263 ± 0.03

5.296 ± 0.019

5.96 ± 0.022

8.68 ± 0.031

1.503 ± 0.012

2.202 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski