Sophora japonica powdery mildew-associated partitivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3IZM6|A0A1B3IZM6_9VIRU Putative coat protein OS=Sophora japonica powdery mildew-associated partitivirus OX=1891704 PE=4 SV=1
MM1 pKa = 7.38SGKK4 pKa = 7.88STKK7 pKa = 9.9KK8 pKa = 10.09SSSSKK13 pKa = 10.52SRR15 pKa = 11.84DD16 pKa = 3.26FRR18 pKa = 11.84KK19 pKa = 9.84EE20 pKa = 3.32AGRR23 pKa = 11.84PSLKK27 pKa = 10.24KK28 pKa = 10.65EE29 pKa = 3.85PVLSSGEE36 pKa = 4.18SSDD39 pKa = 4.25DD40 pKa = 3.62SSDD43 pKa = 4.09AEE45 pKa = 4.22IEE47 pKa = 4.1QALDD51 pKa = 3.33IPVKK55 pKa = 10.6DD56 pKa = 3.92DD57 pKa = 3.2SRR59 pKa = 11.84RR60 pKa = 11.84SKK62 pKa = 9.17STKK65 pKa = 9.05GKK67 pKa = 8.8QRR69 pKa = 11.84SVASRR74 pKa = 11.84EE75 pKa = 4.09KK76 pKa = 10.41KK77 pKa = 9.83QKK79 pKa = 10.07QKK81 pKa = 10.89PSVILADD88 pKa = 5.12DD89 pKa = 4.24DD90 pKa = 4.29TDD92 pKa = 5.7DD93 pKa = 4.85EE94 pKa = 6.02ANDD97 pKa = 3.85DD98 pKa = 4.02LAKK101 pKa = 10.6KK102 pKa = 10.21SEE104 pKa = 4.49SKK106 pKa = 10.44VAPHH110 pKa = 6.06MVLITGSTYY119 pKa = 10.49HH120 pKa = 6.55FGFPPVTHH128 pKa = 7.42PATSTFVPWCGNFFSVLFFMGEE150 pKa = 4.18SLCNSTLVQEE160 pKa = 4.87SIPDD164 pKa = 3.45YY165 pKa = 8.19FTPVLYY171 pKa = 9.58WYY173 pKa = 10.67GSMIFILQILRR184 pKa = 11.84ARR186 pKa = 11.84EE187 pKa = 3.54AAGAITRR194 pKa = 11.84DD195 pKa = 3.56EE196 pKa = 4.2RR197 pKa = 11.84TVLQMIIKK205 pKa = 8.33TYY207 pKa = 10.13PLEE210 pKa = 3.93QWTVLSPMIGFLQSLGSVKK229 pKa = 9.35PTDD232 pKa = 3.77PMYY235 pKa = 11.3SWICPAFPPFTGFTALHH252 pKa = 7.14SYY254 pKa = 10.65RR255 pKa = 11.84GMSTVPGIQRR265 pKa = 11.84MPPILAYY272 pKa = 10.28QKK274 pKa = 10.68FIFNFGAHH282 pKa = 4.74TTQTRR287 pKa = 11.84DD288 pKa = 3.0AFVYY292 pKa = 7.26PQPTITPQNQFVGLQGFGANDD313 pKa = 4.61DD314 pKa = 4.91DD315 pKa = 5.13FQCLTNMYY323 pKa = 9.44GWVSPPEE330 pKa = 4.22TGSLITVTDD339 pKa = 4.38LNLKK343 pKa = 9.67IRR345 pKa = 11.84TIRR348 pKa = 11.84RR349 pKa = 11.84WAVPDD354 pKa = 3.42VPNNLTVTNLKK365 pKa = 9.15TFMGLSDD372 pKa = 4.93DD373 pKa = 6.07LSLSWMFRR381 pKa = 11.84VLRR384 pKa = 11.84QMNSLSNFFPDD395 pKa = 3.34STNLANISPTTGLSSIADD413 pKa = 3.57VDD415 pKa = 3.46IGRR418 pKa = 11.84APARR422 pKa = 11.84IATDD426 pKa = 2.25SWYY429 pKa = 10.39RR430 pKa = 11.84GRR432 pKa = 11.84KK433 pKa = 9.23GITINILSPAGFDD446 pKa = 4.37DD447 pKa = 4.4SSQLRR452 pKa = 11.84LSAATCFNASLADD465 pKa = 3.88STHH468 pKa = 6.35SPLGRR473 pKa = 11.84QISSLQSGPYY483 pKa = 9.1FVNDD487 pKa = 3.78DD488 pKa = 4.24EE489 pKa = 4.76EE490 pKa = 4.69HH491 pKa = 6.69VSIPLIQYY499 pKa = 9.1EE500 pKa = 4.43SGARR504 pKa = 11.84TDD506 pKa = 3.32PVLRR510 pKa = 11.84FSEE513 pKa = 4.46IIEE516 pKa = 4.18SKK518 pKa = 10.89LFDD521 pKa = 3.91RR522 pKa = 11.84NGGRR526 pKa = 11.84KK527 pKa = 8.78

Molecular weight: 58.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3IZM6|A0A1B3IZM6_9VIRU Putative coat protein OS=Sophora japonica powdery mildew-associated partitivirus OX=1891704 PE=4 SV=1
MM1 pKa = 7.85ASTNLLRR8 pKa = 11.84LGKK11 pKa = 9.84IPRR14 pKa = 11.84SKK16 pKa = 10.5KK17 pKa = 10.19HH18 pKa = 6.25NDD20 pKa = 3.24PLSRR24 pKa = 11.84SLRR27 pKa = 11.84INRR30 pKa = 11.84IRR32 pKa = 11.84QGIIKK37 pKa = 9.97RR38 pKa = 11.84AIYY41 pKa = 9.36KK42 pKa = 9.74ICPINLARR50 pKa = 11.84QVIFGFKK57 pKa = 9.87RR58 pKa = 11.84SEE60 pKa = 4.15GSDD63 pKa = 3.3DD64 pKa = 3.61VAEE67 pKa = 4.17TDD69 pKa = 4.05FLRR72 pKa = 11.84SDD74 pKa = 3.5VPYY77 pKa = 10.93FDD79 pKa = 4.25MKK81 pKa = 10.94RR82 pKa = 11.84DD83 pKa = 3.66FHH85 pKa = 6.41YY86 pKa = 10.99LRR88 pKa = 11.84ALRR91 pKa = 11.84VCEE94 pKa = 3.73RR95 pKa = 11.84LFRR98 pKa = 11.84PSRR101 pKa = 11.84TLHH104 pKa = 6.58PIAFPDD110 pKa = 3.46LRR112 pKa = 11.84FYY114 pKa = 10.48PWSLSVSAEE123 pKa = 3.9APFSVEE129 pKa = 4.12KK130 pKa = 10.64KK131 pKa = 9.59RR132 pKa = 11.84STLIRR137 pKa = 11.84SRR139 pKa = 11.84QSDD142 pKa = 3.97GEE144 pKa = 4.22DD145 pKa = 3.19LDD147 pKa = 4.85GRR149 pKa = 11.84LTFHH153 pKa = 6.8NLYY156 pKa = 10.95NEE158 pKa = 3.79IFEE161 pKa = 4.97LNRR164 pKa = 11.84NLIHH168 pKa = 6.89QIKK171 pKa = 10.33EE172 pKa = 3.78GDD174 pKa = 3.21KK175 pKa = 11.3SFWNKK180 pKa = 10.72DD181 pKa = 3.45GTPRR185 pKa = 11.84PYY187 pKa = 10.04WYY189 pKa = 7.9NTLHH193 pKa = 5.92TRR195 pKa = 11.84PHH197 pKa = 6.0LVKK200 pKa = 10.71SSEE203 pKa = 3.95PDD205 pKa = 2.76KK206 pKa = 11.12LRR208 pKa = 11.84AVFGVPKK215 pKa = 10.18LLLMAEE221 pKa = 4.35NMFIWSLQKK230 pKa = 10.61EE231 pKa = 4.39YY232 pKa = 11.0LNQKK236 pKa = 8.42IQSPMLWGFEE246 pKa = 4.18TFKK249 pKa = 11.3GGWLKK254 pKa = 10.15IWNRR258 pKa = 11.84MYY260 pKa = 10.96SKK262 pKa = 10.55KK263 pKa = 10.54CSTFLSADD271 pKa = 2.53WSGFDD276 pKa = 3.3RR277 pKa = 11.84FALFEE282 pKa = 4.35CVDD285 pKa = 4.78DD286 pKa = 3.73IHH288 pKa = 8.73RR289 pKa = 11.84MWRR292 pKa = 11.84NWFDD296 pKa = 3.41FSKK299 pKa = 10.96YY300 pKa = 9.8EE301 pKa = 3.87PTIAEE306 pKa = 4.26SGPPGIQLSYY316 pKa = 10.26PKK318 pKa = 10.63SKK320 pKa = 9.87TNPQKK325 pKa = 10.36IEE327 pKa = 4.07RR328 pKa = 11.84LWNWMCYY335 pKa = 8.52CSKK338 pKa = 9.36YY339 pKa = 9.54TPIKK343 pKa = 9.1GQSGQLYY350 pKa = 7.49QWQYY354 pKa = 10.61NGIASGYY361 pKa = 8.53QQTQLIGSFVNSIYY375 pKa = 10.44MLTCLSDD382 pKa = 3.4LGINIEE388 pKa = 4.17SDD390 pKa = 2.99NFQLFVQGDD399 pKa = 3.63DD400 pKa = 3.54SLTEE404 pKa = 3.99FSEE407 pKa = 5.06IIQKK411 pKa = 10.76DD412 pKa = 4.02DD413 pKa = 3.62LPKK416 pKa = 10.68FLTNLAKK423 pKa = 10.26VAKK426 pKa = 10.2SRR428 pKa = 11.84FNANLSVQKK437 pKa = 7.84TTAGEE442 pKa = 4.1SLNDD446 pKa = 3.76VEE448 pKa = 4.8VLSYY452 pKa = 11.59NNTYY456 pKa = 10.88GIAFRR461 pKa = 11.84DD462 pKa = 3.7EE463 pKa = 4.62AEE465 pKa = 4.33LLAHH469 pKa = 7.07LLYY472 pKa = 10.28PEE474 pKa = 5.03RR475 pKa = 11.84FQTLEE480 pKa = 4.07ATVSCCIGIAYY491 pKa = 10.22ASMGCSQYY499 pKa = 11.19VDD501 pKa = 3.99DD502 pKa = 4.95TCLDD506 pKa = 3.53AYY508 pKa = 11.36NFLTTQFKK516 pKa = 10.69VKK518 pKa = 9.77PDD520 pKa = 3.62SNFLQDD526 pKa = 3.16FFRR529 pKa = 11.84IRR531 pKa = 11.84GSPLTDD537 pKa = 3.93DD538 pKa = 4.0FHH540 pKa = 8.6NPRR543 pKa = 11.84FPTKK547 pKa = 10.47DD548 pKa = 2.68EE549 pKa = 4.58CFNQNYY555 pKa = 7.11EE556 pKa = 3.88VRR558 pKa = 11.84SRR560 pKa = 11.84SDD562 pKa = 3.24SEE564 pKa = 4.3KK565 pKa = 9.91QRR567 pKa = 11.84LWPSIPTGDD576 pKa = 3.79YY577 pKa = 10.1GFHH580 pKa = 7.6FINEE584 pKa = 4.0

Molecular weight: 68.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1111

527

584

555.5

63.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.041 ± 0.594

1.53 ± 0.411

6.571 ± 0.184

4.41 ± 0.569

6.301 ± 0.429

5.491 ± 0.411

1.62 ± 0.206

6.031 ± 0.029

5.581 ± 0.457

8.821 ± 0.601

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.28

4.59 ± 0.696

5.851 ± 0.692

4.32 ± 0.103

6.121 ± 0.436

9.991 ± 1.119

6.031 ± 0.967

4.23 ± 0.631

1.98 ± 0.326

3.42 ± 0.673
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski