Cylindrospermopsis raciborskii virus RM-2018a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346A690|A0A346A690_9CAUD Uncharacterized protein OS=Cylindrospermopsis raciborskii virus RM-2018a OX=2293728 GN=CrV_gp125 PE=4 SV=1
MM1 pKa = 7.77SNQQNLTQVLSDD13 pKa = 4.53LYY15 pKa = 11.48LNGIDD20 pKa = 3.83TTNMTLEE27 pKa = 3.97EE28 pKa = 4.84AMEE31 pKa = 4.01KK32 pKa = 8.32WQEE35 pKa = 3.86VVLQYY40 pKa = 10.78QSEE43 pKa = 4.36EE44 pKa = 3.92

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346A668|A0A346A668_9CAUD Uncharacterized protein OS=Cylindrospermopsis raciborskii virus RM-2018a OX=2293728 GN=CrV_gp088 PE=4 SV=1
MM1 pKa = 7.86PNQEE5 pKa = 3.88TLNRR9 pKa = 11.84KK10 pKa = 8.49KK11 pKa = 10.62ARR13 pKa = 11.84KK14 pKa = 9.8AEE16 pKa = 4.08VARR19 pKa = 11.84LLLAGMTQTAIAQIYY34 pKa = 8.44GLSVSCISLYY44 pKa = 10.71AKK46 pKa = 10.21EE47 pKa = 3.97MQIPCKK53 pKa = 10.26ADD55 pKa = 2.76WYY57 pKa = 9.74VSRR60 pKa = 11.84NKK62 pKa = 10.2ILDD65 pKa = 3.29LHH67 pKa = 5.95RR68 pKa = 11.84QGLRR72 pKa = 11.84QSEE75 pKa = 4.51IARR78 pKa = 11.84KK79 pKa = 8.8LALSRR84 pKa = 11.84QRR86 pKa = 11.84VSEE89 pKa = 4.23IIKK92 pKa = 10.56KK93 pKa = 9.49FAA95 pKa = 4.12

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

26231

41

4416

262.3

29.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.342 ± 0.345

0.923 ± 0.163

4.826 ± 0.183

6.306 ± 0.319

4.068 ± 0.156

6.618 ± 0.303

1.243 ± 0.132

6.847 ± 0.177

6.328 ± 0.473

8.947 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.107

6.157 ± 0.255

4.277 ± 0.172

4.232 ± 0.3

4.479 ± 0.157

7.243 ± 0.303

6.999 ± 0.451

6.401 ± 0.196

1.407 ± 0.204

3.698 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski