Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316) (Mycobacterium thermoresistibile)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium thermoresistibile

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7CNR1|G7CNR1_MYCT3 Oxidoreductase 2-nitropropane dioxygenase OS=Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316) OX=1078020 GN=KEK_23306 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.21AVRR5 pKa = 11.84DD6 pKa = 4.06HH7 pKa = 7.15LPPGLPPDD15 pKa = 4.57PFADD19 pKa = 4.41DD20 pKa = 4.67PYY22 pKa = 11.51DD23 pKa = 3.6PARR26 pKa = 11.84ALDD29 pKa = 4.01EE30 pKa = 4.96LEE32 pKa = 4.8PGQPLDD38 pKa = 3.58PQEE41 pKa = 4.26RR42 pKa = 11.84LAVEE46 pKa = 4.55ADD48 pKa = 3.56LADD51 pKa = 3.99LAVYY55 pKa = 8.38EE56 pKa = 4.79ALLSPKK62 pKa = 9.71GVRR65 pKa = 11.84GLVVCCDD72 pKa = 3.57EE73 pKa = 4.53CQQDD77 pKa = 4.66HH78 pKa = 5.22YY79 pKa = 11.02HH80 pKa = 6.67DD81 pKa = 4.0WDD83 pKa = 3.57MLRR86 pKa = 11.84ANLLQLLVDD95 pKa = 4.02GTVRR99 pKa = 11.84PHH101 pKa = 6.27EE102 pKa = 4.31PAYY105 pKa = 10.66DD106 pKa = 4.13PEE108 pKa = 6.04PDD110 pKa = 5.08AYY112 pKa = 9.99VTWDD116 pKa = 3.35YY117 pKa = 11.62CRR119 pKa = 11.84GYY121 pKa = 11.36ADD123 pKa = 4.13AVLNEE128 pKa = 4.06ATSDD132 pKa = 3.68DD133 pKa = 3.85FRR135 pKa = 6.62

Molecular weight: 15.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7CIQ3|G7CIQ3_MYCT3 Peptidyl-prolyl cis-trans isomerase OS=Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316) OX=1078020 GN=KEK_10823 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSNRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.46GRR42 pKa = 11.84RR43 pKa = 11.84ALTAA47 pKa = 3.88

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4613

0

4613

1469702

30

3089

318.6

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.932 ± 0.048

0.8 ± 0.01

6.438 ± 0.025

5.626 ± 0.033

2.959 ± 0.018

8.904 ± 0.036

2.198 ± 0.019

4.195 ± 0.025

1.952 ± 0.025

9.776 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.014

2.087 ± 0.019

6.15 ± 0.034

2.877 ± 0.019

7.868 ± 0.04

4.851 ± 0.02

6.086 ± 0.022

8.696 ± 0.031

1.485 ± 0.016

2.106 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski