Mycobacterium phage Makemake

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1PCB6|A0A1B1PCB6_9CAUD Integrase OS=Mycobacterium phage Makemake OX=1873889 GN=38 PE=4 SV=1
MM1 pKa = 7.59GFGLNSICWGAWQGWVGNLGGYY23 pKa = 9.1IAVIFYY29 pKa = 10.54NSEE32 pKa = 4.22DD33 pKa = 3.66GSVIAVTLNNFSAHH47 pKa = 5.36VDD49 pKa = 3.51AVDD52 pKa = 4.11LFYY55 pKa = 11.13QIAYY59 pKa = 9.85LLNPEE64 pKa = 4.45STGHH68 pKa = 6.67RR69 pKa = 11.84DD70 pKa = 2.94WIFRR74 pKa = 11.84PDD76 pKa = 3.55PAEE79 pKa = 4.45DD80 pKa = 3.6ADD82 pKa = 4.07EE83 pKa = 4.53VRR85 pKa = 11.84DD86 pKa = 3.62PTLYY90 pKa = 9.89LTVEE94 pKa = 4.33STGDD98 pKa = 3.38NQIPADD104 pKa = 3.87VPFEE108 pKa = 3.98II109 pKa = 5.71

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1PC87|A0A1B1PC87_9CAUD Uncharacterized protein OS=Mycobacterium phage Makemake OX=1873889 GN=8 PE=4 SV=1
MM1 pKa = 7.23GWEE4 pKa = 3.86SSDD7 pKa = 3.24RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.33RR11 pKa = 11.84LPADD15 pKa = 3.28WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.46RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.29PDD37 pKa = 3.72ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.06VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.5EE54 pKa = 4.68SSPLQAACRR63 pKa = 11.84SCHH66 pKa = 4.97GRR68 pKa = 11.84KK69 pKa = 8.93SAQEE73 pKa = 4.02GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.87PGRR95 pKa = 11.84RR96 pKa = 11.84SNN98 pKa = 3.52

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16662

23

903

179.2

19.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.903 ± 0.405

0.804 ± 0.117

6.626 ± 0.193

6.536 ± 0.256

3.223 ± 0.143

8.918 ± 0.607

1.903 ± 0.175

4.801 ± 0.24

4.111 ± 0.225

8.546 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.017 ± 0.128

3.205 ± 0.147

5.528 ± 0.203

3.247 ± 0.143

6.182 ± 0.343

6.008 ± 0.292

6.416 ± 0.279

7.268 ± 0.208

1.963 ± 0.125

2.797 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski