Human gut microviridae SH-CHD8

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9Q118|A0A1X9Q118_9VIRU Putative VP4 OS=Human gut microviridae SH-CHD8 OX=1986033 PE=4 SV=1
MM1 pKa = 7.56SNFSKK6 pKa = 10.49ISLGISTKK14 pKa = 10.22RR15 pKa = 11.84YY16 pKa = 5.98THH18 pKa = 7.45DD19 pKa = 3.13MSFDD23 pKa = 3.77NNTTLSFGVCQPLFCQILNDD43 pKa = 3.85KK44 pKa = 10.8DD45 pKa = 3.63KK46 pKa = 11.24LAGNIRR52 pKa = 11.84QLVRR56 pKa = 11.84LAPLPVPSFGRR67 pKa = 11.84MRR69 pKa = 11.84LVNKK73 pKa = 10.03LRR75 pKa = 11.84FVQMTDD81 pKa = 2.14ICPYY85 pKa = 9.3YY86 pKa = 10.59EE87 pKa = 4.58AMLSGAYY94 pKa = 10.58VNTSDD99 pKa = 5.82LNYY102 pKa = 10.48LPKK105 pKa = 10.55EE106 pKa = 4.05VLNDD110 pKa = 3.87SIKK113 pKa = 11.03VLTQMVLQYY122 pKa = 11.41SVFDD126 pKa = 3.91YY127 pKa = 10.77FKK129 pKa = 11.04VDD131 pKa = 3.61ASSSTPKK138 pKa = 10.47YY139 pKa = 10.02VYY141 pKa = 10.65QNVLTPSVLNEE152 pKa = 3.83ALSFYY157 pKa = 10.73HH158 pKa = 6.94LVVRR162 pKa = 11.84DD163 pKa = 3.66NTEE166 pKa = 3.14ISAAIPDD173 pKa = 4.37PKK175 pKa = 10.45PHH177 pKa = 6.6GGNVNTAISPDD188 pKa = 3.47SADD191 pKa = 3.66YY192 pKa = 11.0LISSDD197 pKa = 4.61SALICCRR204 pKa = 11.84LTEE207 pKa = 4.12RR208 pKa = 11.84GKK210 pKa = 10.39RR211 pKa = 11.84LRR213 pKa = 11.84NVLVGLGYY221 pKa = 11.04ALDD224 pKa = 3.99VSCTDD229 pKa = 3.55SVSLLPLFAYY239 pKa = 9.33FKK241 pKa = 10.81AWYY244 pKa = 9.5DD245 pKa = 3.23SYY247 pKa = 11.81APSRR251 pKa = 11.84EE252 pKa = 4.32TQWNQTNAFVLIDD265 pKa = 3.62ACFQNYY271 pKa = 7.33LTTLSGFTTANGSTQKK287 pKa = 9.86VQKK290 pKa = 10.51AVFGFFEE297 pKa = 4.81DD298 pKa = 5.47LANTWYY304 pKa = 10.23VAKK307 pKa = 10.25DD308 pKa = 3.79DD309 pKa = 4.05YY310 pKa = 11.88VSAHH314 pKa = 7.05RR315 pKa = 11.84LNAVSSNTDD324 pKa = 2.63SFGDD328 pKa = 3.72VQNKK332 pKa = 9.26IPSDD336 pKa = 3.83NNLTTLQGVKK346 pKa = 10.82YY347 pKa = 9.45NGNGNVPEE355 pKa = 4.19ATVVTDD361 pKa = 4.55FALQSLMRR369 pKa = 11.84ISKK372 pKa = 9.54FINKK376 pKa = 8.37DD377 pKa = 3.26TVIGKK382 pKa = 9.29KK383 pKa = 10.25VSTWLKK389 pKa = 8.83AHH391 pKa = 6.87FGADD395 pKa = 3.22VASSFFKK402 pKa = 10.89DD403 pKa = 3.83SVSLPDD409 pKa = 3.68VVVSCNINDD418 pKa = 3.84VFSTADD424 pKa = 3.07TSTSDD429 pKa = 3.86GSGEE433 pKa = 3.96QLGSYY438 pKa = 9.08AGKK441 pKa = 10.7GLGFGDD447 pKa = 4.03GSFSFTAPTFGYY459 pKa = 9.49FVVVSCLVPDD469 pKa = 3.83SKK471 pKa = 11.37YY472 pKa = 10.64FQGVDD477 pKa = 3.35PSLFGLDD484 pKa = 4.59RR485 pKa = 11.84FTLPNADD492 pKa = 4.43FDD494 pKa = 4.24ALSYY498 pKa = 10.79EE499 pKa = 3.92ITPCSFLCAHH509 pKa = 6.82NDD511 pKa = 3.46MYY513 pKa = 11.76VSGQKK518 pKa = 8.58STSDD522 pKa = 3.03KK523 pKa = 11.23GFGFVPRR530 pKa = 11.84YY531 pKa = 10.24SGFKK535 pKa = 8.1TRR537 pKa = 11.84RR538 pKa = 11.84NIVNGDD544 pKa = 3.15MSRR547 pKa = 11.84RR548 pKa = 11.84GTIASYY554 pKa = 10.88SPYY557 pKa = 10.58YY558 pKa = 9.88LDD560 pKa = 6.16KK561 pKa = 10.92ILTEE565 pKa = 3.71NSFRR569 pKa = 11.84SEE571 pKa = 3.84KK572 pKa = 10.63NADD575 pKa = 3.01GSYY578 pKa = 9.74TFVAVNNPLPLASEE592 pKa = 4.38VWRR595 pKa = 11.84FPTRR599 pKa = 11.84YY600 pKa = 9.26SWLGNFNRR608 pKa = 11.84IFYY611 pKa = 10.15NSGSAVFDD619 pKa = 4.3DD620 pKa = 4.76ASSSDD625 pKa = 3.62EE626 pKa = 4.23VNDD629 pKa = 3.76FTITPIDD636 pKa = 3.92DD637 pKa = 3.66NFIVQSVISMKK648 pKa = 9.61LTNALKK654 pKa = 9.86PIAMSYY660 pKa = 10.84DD661 pKa = 3.57VYY663 pKa = 11.58DD664 pKa = 5.13DD665 pKa = 5.67DD666 pKa = 5.59VDD668 pKa = 4.0NSSKK672 pKa = 10.09TITQEE677 pKa = 3.51

Molecular weight: 74.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9Q123|A0A1X9Q123_9VIRU Uncharacterized protein OS=Human gut microviridae SH-CHD8 OX=1986033 PE=4 SV=1
MM1 pKa = 7.8NYY3 pKa = 9.35PLPIVICLLILFMMNWSFSMGFCLNFLSKK32 pKa = 10.54FVMNKK37 pKa = 8.66EE38 pKa = 4.08VIKK41 pKa = 10.62IIIKK45 pKa = 9.18VVIYY49 pKa = 10.78ALGLIAAYY57 pKa = 10.46LGVTSLSSCTFSRR70 pKa = 11.84SSVAEE75 pKa = 3.92GRR77 pKa = 11.84ATIITTDD84 pKa = 3.26TTFISHH90 pKa = 6.38QGVLKK95 pKa = 10.18TKK97 pKa = 10.47

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1677

97

677

419.3

47.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.633 ± 1.96

1.968 ± 0.611

6.38 ± 0.79

3.637 ± 0.86

5.725 ± 0.919

5.605 ± 0.462

1.491 ± 0.488

4.651 ± 0.687

6.321 ± 0.737

9.064 ± 0.514

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.362

6.5 ± 0.288

3.399 ± 0.505

3.936 ± 1.055

4.055 ± 0.473

9.362 ± 1.001

5.486 ± 0.837

6.44 ± 1.238

0.894 ± 0.022

5.188 ± 0.885
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski