Prauserella muralis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Prauserella

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V4AM13|A0A2V4AM13_9PSEU Uncharacterized protein OS=Prauserella muralis OX=588067 GN=BAY60_28255 PE=3 SV=1
MM1 pKa = 7.33PAKK4 pKa = 10.59DD5 pKa = 3.4EE6 pKa = 5.04GIAMTYY12 pKa = 9.66PPQPGQQPGWNGYY25 pKa = 9.13GGQPPQGGCPQGYY38 pKa = 6.89QQHH41 pKa = 6.62GGHH44 pKa = 6.73PHH46 pKa = 5.51QGGYY50 pKa = 9.22PPPGQVPPYY59 pKa = 9.87GYY61 pKa = 9.17PQGGGGWGGPPPPKK75 pKa = 10.25SRR77 pKa = 11.84AGLWAGLGAGALAVVAFVITAFVAPGFLLGDD108 pKa = 4.18DD109 pKa = 4.56SGDD112 pKa = 3.66PPGGAGAQAGGQGGGNSAEE131 pKa = 4.4AFAQGIVAAFNNSDD145 pKa = 3.82TNTLNAAMCPGAEE158 pKa = 4.0PDD160 pKa = 3.34VSEE163 pKa = 5.07VIGQASVVEE172 pKa = 4.02NMKK175 pKa = 10.65LGAVKK180 pKa = 10.37SVSDD184 pKa = 3.75TEE186 pKa = 4.17ATAEE190 pKa = 4.14VSLTAAGSPMTAVAALKK207 pKa = 10.89NEE209 pKa = 4.02GGNWCWDD216 pKa = 4.07GITMGSAGSSPSVGASEE233 pKa = 4.87SSSAPAPGTGGGDD246 pKa = 3.4PDD248 pKa = 3.85GVVSRR253 pKa = 11.84FVEE256 pKa = 4.32AVNNGDD262 pKa = 3.5KK263 pKa = 10.49NAAMSLVCPDD273 pKa = 3.15QEE275 pKa = 4.61YY276 pKa = 8.39TVGTDD281 pKa = 2.94VEE283 pKa = 4.33RR284 pKa = 11.84AIGDD288 pKa = 4.0SAKK291 pKa = 9.66LTAGPAEE298 pKa = 4.18QDD300 pKa = 3.17GSVYY304 pKa = 9.19TAEE307 pKa = 4.24VTGTDD312 pKa = 2.98DD313 pKa = 4.03DD314 pKa = 4.36GRR316 pKa = 11.84VEE318 pKa = 4.22GAVGVSDD325 pKa = 4.43DD326 pKa = 3.86GCVIGLVLFF335 pKa = 4.81

Molecular weight: 32.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V4ADL0|A0A2V4ADL0_9PSEU FAD-dependent oxidoreductase OS=Prauserella muralis OX=588067 GN=BAY60_34510 PE=4 SV=1
MM1 pKa = 6.49STVRR5 pKa = 11.84FRR7 pKa = 11.84WRR9 pKa = 11.84KK10 pKa = 4.76TLRR13 pKa = 11.84WGPIRR18 pKa = 11.84LNTHH22 pKa = 6.06NGRR25 pKa = 11.84ATSWSFTLGRR35 pKa = 11.84LSWNSRR41 pKa = 11.84TGWHH45 pKa = 7.22LDD47 pKa = 3.37TPGPGSVHH55 pKa = 6.41IGHH58 pKa = 7.92PGRR61 pKa = 11.84TRR63 pKa = 11.84PRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 3.13

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7018

0

7018

2183359

29

4480

311.1

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.622 ± 0.048

0.784 ± 0.008

5.909 ± 0.023

5.853 ± 0.028

2.792 ± 0.015

9.41 ± 0.028

2.281 ± 0.015

3.301 ± 0.018

1.758 ± 0.02

10.675 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.011

1.738 ± 0.013

5.915 ± 0.026

2.775 ± 0.014

8.334 ± 0.031

4.843 ± 0.022

5.905 ± 0.018

8.93 ± 0.021

1.496 ± 0.012

1.992 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski