Tortoise microvirus 90

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W7V6|A0A4P8W7V6_9VIRU DNA pilot protein OS=Tortoise microvirus 90 OX=2583199 PE=4 SV=1
MM1 pKa = 7.15STTFALGEE9 pKa = 3.95QSTDD13 pKa = 3.32LEE15 pKa = 4.36KK16 pKa = 11.18SGGISQTVPDD26 pKa = 3.66QSLSLRR32 pKa = 11.84EE33 pKa = 3.88LVTRR37 pKa = 11.84QSQGLPIPMKK47 pKa = 10.6HH48 pKa = 6.69PIFSDD53 pKa = 3.67QEE55 pKa = 3.88IPDD58 pKa = 4.03LKK60 pKa = 10.97KK61 pKa = 10.73LDD63 pKa = 3.76LAEE66 pKa = 4.1IQEE69 pKa = 4.53LKK71 pKa = 10.43EE72 pKa = 3.99VAASQVNSLRR82 pKa = 11.84QKK84 pKa = 10.11LQQAEE89 pKa = 4.11LAEE92 pKa = 4.32RR93 pKa = 11.84QAVLAEE99 pKa = 4.14KK100 pKa = 10.42QKK102 pKa = 11.09NLDD105 pKa = 3.64KK106 pKa = 10.98AASAANQPPPAPPAQNSGVAATT128 pKa = 4.33

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WAB2|A0A4P8WAB2_9VIRU Major capsid protein OS=Tortoise microvirus 90 OX=2583199 PE=3 SV=1
MM1 pKa = 7.54ACDD4 pKa = 4.43FPYY7 pKa = 10.48SIQNPTWPLRR17 pKa = 11.84SKK19 pKa = 9.76TKK21 pKa = 10.5FIPVPCGKK29 pKa = 10.33CPEE32 pKa = 4.24CCMRR36 pKa = 11.84RR37 pKa = 11.84SNNWQFRR44 pKa = 11.84LLQQEE49 pKa = 4.44KK50 pKa = 9.71ASSSASFVTLTYY62 pKa = 9.88NTDD65 pKa = 3.33HH66 pKa = 7.4VPMSKK71 pKa = 9.84RR72 pKa = 11.84GFMTLCKK79 pKa = 9.89TDD81 pKa = 3.79FQDD84 pKa = 3.21FMKK87 pKa = 10.52RR88 pKa = 11.84LRR90 pKa = 11.84YY91 pKa = 8.1YY92 pKa = 9.9HH93 pKa = 6.92PKK95 pKa = 9.28GAKK98 pKa = 8.65LRR100 pKa = 11.84YY101 pKa = 8.39YY102 pKa = 10.97AVGEE106 pKa = 4.24YY107 pKa = 10.57GSKK110 pKa = 8.61TFRR113 pKa = 11.84PHH115 pKa = 4.42FHH117 pKa = 7.28AIIFDD122 pKa = 4.28LDD124 pKa = 3.67RR125 pKa = 11.84EE126 pKa = 4.28QLPKK130 pKa = 10.7AWTLGDD136 pKa = 3.2IHH138 pKa = 8.54IGDD141 pKa = 3.62VSGASVAYY149 pKa = 4.99TTKK152 pKa = 10.71YY153 pKa = 6.42MHH155 pKa = 7.07KK156 pKa = 10.04GKK158 pKa = 9.65MIPVHH163 pKa = 6.83SNDD166 pKa = 3.15DD167 pKa = 4.19RR168 pKa = 11.84IPEE171 pKa = 4.02FSLMSKK177 pKa = 10.63KK178 pKa = 10.63LGANYY183 pKa = 7.76LTPQMVAYY191 pKa = 9.45HH192 pKa = 5.65QADD195 pKa = 3.59LTRR198 pKa = 11.84NYY200 pKa = 8.46VTLEE204 pKa = 4.13GGVKK208 pKa = 9.82VAMPRR213 pKa = 11.84YY214 pKa = 8.29YY215 pKa = 10.1RR216 pKa = 11.84EE217 pKa = 4.13KK218 pKa = 10.15IYY220 pKa = 11.01SEE222 pKa = 4.08EE223 pKa = 3.87QRR225 pKa = 11.84NLQNLHH231 pKa = 5.49NEE233 pKa = 4.04KK234 pKa = 10.15LAKK237 pKa = 9.96SALADD242 pKa = 3.54AEE244 pKa = 4.26EE245 pKa = 4.89SYY247 pKa = 10.97HH248 pKa = 6.71RR249 pKa = 11.84RR250 pKa = 11.84TGTSDD255 pKa = 3.43FEE257 pKa = 4.44RR258 pKa = 11.84ADD260 pKa = 3.6FEE262 pKa = 4.22ARR264 pKa = 11.84KK265 pKa = 10.03AKK267 pKa = 10.61LEE269 pKa = 3.92NFRR272 pKa = 11.84KK273 pKa = 9.78RR274 pKa = 11.84ARR276 pKa = 11.84DD277 pKa = 3.42GRR279 pKa = 11.84KK280 pKa = 9.9SII282 pKa = 4.29

Molecular weight: 32.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1235

128

519

308.8

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.854 ± 1.069

0.567 ± 0.427

5.668 ± 0.24

5.911 ± 0.415

3.968 ± 1.076

6.316 ± 1.04

2.51 ± 0.816

5.263 ± 0.767

5.911 ± 0.82

8.259 ± 1.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.277

5.02 ± 0.785

5.668 ± 0.709

5.425 ± 1.477

5.83 ± 0.899

7.935 ± 1.212

5.182 ± 0.36

4.858 ± 0.644

0.81 ± 0.272

4.372 ± 0.976
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski