Mycobacterium phage Onyinye

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L7K5|A0A6B9L7K5_9CAUD DNA primase/polymerase OS=Mycobacterium phage Onyinye OX=2686235 GN=107 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 6.18IINPGYY8 pKa = 10.19HH9 pKa = 4.85EE10 pKa = 5.98HH11 pKa = 6.78RR12 pKa = 11.84PQWEE16 pKa = 4.4GNMKK20 pKa = 9.61VCQDD24 pKa = 3.12CRR26 pKa = 11.84QIIFEE31 pKa = 5.28PPTQDD36 pKa = 4.08NNQLLADD43 pKa = 4.42PLEE46 pKa = 4.61TDD48 pKa = 3.65HH49 pKa = 6.75PTGRR53 pKa = 11.84DD54 pKa = 3.29FDD56 pKa = 3.96SLYY59 pKa = 11.24GMRR62 pKa = 11.84VPLDD66 pKa = 3.36YY67 pKa = 11.41LLIRR71 pKa = 11.84MTTEE75 pKa = 4.15LDD77 pKa = 3.06IDD79 pKa = 3.83TPDD82 pKa = 4.2YY83 pKa = 11.17IDD85 pKa = 4.15ILNEE89 pKa = 4.05EE90 pKa = 4.37YY91 pKa = 10.89CGDD94 pKa = 3.65EE95 pKa = 4.08EE96 pKa = 4.27QAEE99 pKa = 4.46NSVPPHH105 pKa = 6.56TYY107 pKa = 10.43NGTVRR112 pKa = 11.84WWVSEE117 pKa = 3.85

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LD05|A0A6B9LD05_9CAUD Uncharacterized protein OS=Mycobacterium phage Onyinye OX=2686235 GN=32 PE=4 SV=1
MM1 pKa = 7.44SKK3 pKa = 10.62SKK5 pKa = 10.8AKK7 pKa = 10.24KK8 pKa = 8.78AAKK11 pKa = 8.62KK12 pKa = 8.39TGSRR16 pKa = 11.84PFASKK21 pKa = 10.33AQARR25 pKa = 11.84LFFANKK31 pKa = 9.9RR32 pKa = 11.84LRR34 pKa = 11.84KK35 pKa = 8.06YY36 pKa = 10.47AKK38 pKa = 9.78KK39 pKa = 9.76KK40 pKa = 8.98AHH42 pKa = 5.57ATGMHH47 pKa = 5.43SAITKK52 pKa = 10.16RR53 pKa = 11.84VGHH56 pKa = 5.88SPAYY60 pKa = 9.7RR61 pKa = 11.84ALPARR66 pKa = 11.84KK67 pKa = 8.96SARR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 9.82ARR74 pKa = 11.84GIKK77 pKa = 9.71KK78 pKa = 10.19

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

23031

36

1741

215.2

23.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.782 ± 0.364

0.877 ± 0.115

6.617 ± 0.269

6.587 ± 0.317

3.243 ± 0.131

8.567 ± 0.415

2.071 ± 0.139

4.854 ± 0.215

5.414 ± 0.517

7.277 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.116

3.903 ± 0.159

5.645 ± 0.19

3.695 ± 0.18

6.274 ± 0.41

5.223 ± 0.185

5.523 ± 0.201

6.813 ± 0.204

1.963 ± 0.115

3.126 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski