Prochlorococcus phage P-SSP7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Tiamatvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q58N24|Q58N24_BPPRP T7-like internal core protein OS=Prochlorococcus phage P-SSP7 OX=268748 GN=16 PE=4 SV=1
MM1 pKa = 7.35ATTEE5 pKa = 4.13HH6 pKa = 7.26FYY8 pKa = 10.66TGNGSTTSFAFTFPYY23 pKa = 9.73LANVDD28 pKa = 3.97VKK30 pKa = 11.51VEE32 pKa = 4.08LDD34 pKa = 3.42NVLKK38 pKa = 10.13TEE40 pKa = 4.11NSSGQTNNDD49 pKa = 3.22YY50 pKa = 10.45TISNTNIVFNSAPGSGVIIHH70 pKa = 7.45IYY72 pKa = 10.55RR73 pKa = 11.84NTNVDD78 pKa = 3.51TAQATYY84 pKa = 10.68AAGSSIRR91 pKa = 11.84AVDD94 pKa = 4.0LNNNQTQVLYY104 pKa = 9.38STQEE108 pKa = 3.73AQSQIIRR115 pKa = 11.84TTDD118 pKa = 2.92IKK120 pKa = 11.17DD121 pKa = 3.33GAVNSTKK128 pKa = 10.03IEE130 pKa = 4.07NNTIVNADD138 pKa = 3.63INSSAAIDD146 pKa = 3.98GSKK149 pKa = 9.75IQASSGSNSGTMSAANFTKK168 pKa = 10.63LGGIEE173 pKa = 4.05TGATADD179 pKa = 3.29QTAAEE184 pKa = 4.19IRR186 pKa = 11.84TLVEE190 pKa = 3.87SASDD194 pKa = 3.71SNVFTDD200 pKa = 4.89ADD202 pKa = 3.74HH203 pKa = 6.75TKK205 pKa = 11.05LNGIEE210 pKa = 4.22SGATADD216 pKa = 3.93QSVSEE221 pKa = 4.64IKK223 pKa = 9.84TLIAGSPLSNTHH235 pKa = 6.02IAADD239 pKa = 3.6ADD241 pKa = 3.7IAHH244 pKa = 6.66SKK246 pKa = 9.97LADD249 pKa = 3.52VTSSQVLIGNASNVPTATSLSGDD272 pKa = 3.49VTLNNAGVVTIANDD286 pKa = 3.36AVEE289 pKa = 4.65IGMIGCEE296 pKa = 3.77QTTITDD302 pKa = 3.88SDD304 pKa = 3.8SHH306 pKa = 7.77LPTSGAVVDD315 pKa = 4.31YY316 pKa = 10.88VSSQLAPIGGLEE328 pKa = 4.14VIANEE333 pKa = 4.01VSFPNTQPAAGVVISITDD351 pKa = 3.2AGGVVFNGSGSSTTGRR367 pKa = 11.84TLGGATVTINNAPTALNSEE386 pKa = 4.48TLVAGAGLMVSSTGSSNIYY405 pKa = 9.88NYY407 pKa = 10.44HH408 pKa = 6.08KK409 pKa = 10.87LFVKK413 pKa = 10.59EE414 pKa = 3.92SDD416 pKa = 3.79VVQLSDD422 pKa = 5.99DD423 pKa = 3.66INDD426 pKa = 3.2FNSRR430 pKa = 11.84YY431 pKa = 9.69RR432 pKa = 11.84IAASAPTSNNDD443 pKa = 3.14EE444 pKa = 4.01GDD446 pKa = 3.89LYY448 pKa = 11.4FDD450 pKa = 3.67TAANKK455 pKa = 9.1MFVYY459 pKa = 10.4DD460 pKa = 4.23GSAWGQVTSTGEE472 pKa = 3.91FKK474 pKa = 10.85ILGIKK479 pKa = 10.56DD480 pKa = 2.97NGQAHH485 pKa = 6.54NGSGPTFNGSNDD497 pKa = 3.32RR498 pKa = 11.84FDD500 pKa = 4.68LFEE503 pKa = 4.38NASAADD509 pKa = 3.54ISQASQLLVVLNGVIQKK526 pKa = 10.23PNDD529 pKa = 3.4GSFSGTEE536 pKa = 3.35EE537 pKa = 4.51GYY539 pKa = 11.33YY540 pKa = 10.53LDD542 pKa = 4.04GTDD545 pKa = 5.5GIRR548 pKa = 11.84FCDD551 pKa = 4.27PPASGSTLFITKK563 pKa = 9.73QGSATQINTPADD575 pKa = 3.56NTITQAKK582 pKa = 8.36IAVDD586 pKa = 3.38AVDD589 pKa = 3.7EE590 pKa = 4.25QRR592 pKa = 11.84LQVSNAPTNGYY603 pKa = 9.02YY604 pKa = 10.56LQAQSGNTGGLTWAAVPNPDD624 pKa = 3.94LTQLSASNLTSGTIPDD640 pKa = 3.53ARR642 pKa = 11.84FPSTLPAVSGANLTGLPPSCTGGGSDD668 pKa = 4.77EE669 pKa = 4.49IFWEE673 pKa = 4.17NGQTVTTNYY682 pKa = 9.98TITNNKK688 pKa = 8.86NAMSAGPITINNGIAVTIGTGEE710 pKa = 3.76NWTIVV715 pKa = 3.21

Molecular weight: 73.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q58N12|Q58N12_BPPRP Gp49 OS=Prochlorococcus phage P-SSP7 OX=268748 GN=PCPG_00046 PE=4 SV=1
MM1 pKa = 7.75AGGAKK6 pKa = 9.35YY7 pKa = 10.99ANGNYY12 pKa = 9.8KK13 pKa = 9.95SQQKK17 pKa = 10.55AYY19 pKa = 10.0NKK21 pKa = 8.3TKK23 pKa = 10.35KK24 pKa = 10.57GLALRR29 pKa = 11.84VNANAINRR37 pKa = 11.84AKK39 pKa = 9.59GTYY42 pKa = 10.39GNGDD46 pKa = 3.76GKK48 pKa = 10.61DD49 pKa = 3.45VAHH52 pKa = 7.32KK53 pKa = 10.43KK54 pKa = 10.32GKK56 pKa = 9.93QNSKK60 pKa = 9.46KK61 pKa = 10.8AKK63 pKa = 10.0DD64 pKa = 3.46ATLQSPSKK72 pKa = 10.23NRR74 pKa = 11.84KK75 pKa = 8.58SRR77 pKa = 11.84LKK79 pKa = 10.06IRR81 pKa = 11.84KK82 pKa = 8.67

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

14200

26

1245

244.8

27.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.113 ± 0.383

1.113 ± 0.172

6.148 ± 0.242

6.113 ± 0.396

3.711 ± 0.175

6.803 ± 0.36

1.775 ± 0.2

6.042 ± 0.214

6.859 ± 0.569

8.197 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.161

5.387 ± 0.368

4.148 ± 0.234

4.204 ± 0.21

4.451 ± 0.264

6.803 ± 0.401

7.211 ± 0.484

5.901 ± 0.307

1.303 ± 0.109

3.479 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski