Microbacterium sp.

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4036 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519H6U1|A0A519H6U1_9MICO ATP-binding cassette domain-containing protein OS=Microbacterium sp. OX=51671 GN=EOO67_01315 PE=4 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84RR4 pKa = 11.84IVPALAATALAVLVLAGCATPAPGAGASDD33 pKa = 4.22APTPADD39 pKa = 3.39PAGQTVPEE47 pKa = 4.37GTGDD51 pKa = 4.2LDD53 pKa = 4.58VDD55 pKa = 4.15AAWLHH60 pKa = 5.83GGSMIAVVTYY70 pKa = 10.51GSSTCLPGVDD80 pKa = 4.96DD81 pKa = 4.61AVLDD85 pKa = 3.94EE86 pKa = 4.63GVLVVSLTPPTGDD99 pKa = 3.1ACTDD103 pKa = 3.68DD104 pKa = 4.15LVPRR108 pKa = 11.84ALAVGAPEE116 pKa = 5.73GIDD119 pKa = 3.22PSAGLEE125 pKa = 4.22VQVLLSDD132 pKa = 4.06QIGTATLAPYY142 pKa = 10.52AGGPAEE148 pKa = 4.82EE149 pKa = 4.59YY150 pKa = 10.79APSAGWAGAEE160 pKa = 4.45HH161 pKa = 6.91IAILTWGSSSCAPSVEE177 pKa = 4.28STTVVSPTEE186 pKa = 3.93VAVTFATPRR195 pKa = 11.84ADD197 pKa = 3.8QVCTMDD203 pKa = 3.41MAPRR207 pKa = 11.84VAVATVDD214 pKa = 3.92GEE216 pKa = 4.4VSRR219 pKa = 11.84DD220 pKa = 3.24ATLTLGGLSLGSPGDD235 pKa = 3.86APASAIPIGG244 pKa = 3.63

Molecular weight: 23.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519GWN5|A0A519GWN5_9MICO DUF3048 domain-containing protein OS=Microbacterium sp. OX=51671 GN=EOO67_08700 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.12TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4036

0

4036

970863

22

1615

240.6

25.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.279 ± 0.047

0.556 ± 0.01

6.524 ± 0.041

5.48 ± 0.037

3.062 ± 0.026

8.929 ± 0.041

2.152 ± 0.019

4.252 ± 0.03

1.981 ± 0.029

10.037 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.016

1.87 ± 0.02

5.39 ± 0.029

2.734 ± 0.02

7.484 ± 0.044

5.483 ± 0.03

6.172 ± 0.032

9.208 ± 0.037

1.549 ± 0.019

2.063 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski