Enterobacteria phage f1 (Bacteriophage f1)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Inovirus; Escherichia virus M13

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7BJW6|A7BJW6_BPF1 IX OS=Enterobacteria phage f1 OX=10863 GN=IX PE=4 SV=1
MM1 pKa = 7.07EE2 pKa = 4.16QVADD6 pKa = 4.42FDD8 pKa = 4.64TIYY11 pKa = 10.92QAMIQISVVLCFALGIIAGGQRR33 pKa = 3.18

Molecular weight: 3.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7BJX1|A7BJX1_BPF1 Gene 4 protein OS=Enterobacteria phage f1 OX=10863 GN=IV PE=3 SV=1
MM1 pKa = 7.44AVYY4 pKa = 9.51FVTGKK9 pKa = 10.48LGSGKK14 pKa = 8.07TLVSVGKK21 pKa = 9.86IQDD24 pKa = 4.07KK25 pKa = 10.55IVAGCKK31 pKa = 9.34IATNLDD37 pKa = 3.28LRR39 pKa = 11.84LQNLPQVGRR48 pKa = 11.84FAKK51 pKa = 9.44TPRR54 pKa = 11.84VLRR57 pKa = 11.84IPDD60 pKa = 3.64KK61 pKa = 11.0PSISDD66 pKa = 3.71LLAIGRR72 pKa = 11.84GNDD75 pKa = 3.55SYY77 pKa = 12.11DD78 pKa = 3.49EE79 pKa = 4.31NKK81 pKa = 10.66NGLLVLDD88 pKa = 4.72EE89 pKa = 4.86CGTWFNTRR97 pKa = 11.84SWNDD101 pKa = 3.27KK102 pKa = 9.17EE103 pKa = 4.09RR104 pKa = 11.84QPIIDD109 pKa = 3.83WFLHH113 pKa = 4.82ARR115 pKa = 11.84KK116 pKa = 9.63LGWDD120 pKa = 3.69IIFLVQDD127 pKa = 3.69LSIVDD132 pKa = 3.64KK133 pKa = 9.99QARR136 pKa = 11.84SALAEE141 pKa = 3.81NVVYY145 pKa = 10.39CRR147 pKa = 11.84RR148 pKa = 11.84LDD150 pKa = 4.36RR151 pKa = 11.84ITLPFVGTLYY161 pKa = 11.3SLITGSKK168 pKa = 8.19MPLPKK173 pKa = 10.44LHH175 pKa = 6.43VGVVKK180 pKa = 11.1YY181 pKa = 10.67GDD183 pKa = 3.9SQLSPTVEE191 pKa = 3.26RR192 pKa = 11.84WLYY195 pKa = 8.69TGKK198 pKa = 9.91NLYY201 pKa = 9.14NAYY204 pKa = 8.11DD205 pKa = 3.67TKK207 pKa = 11.05QAFSSNYY214 pKa = 9.25DD215 pKa = 3.42SGVYY219 pKa = 10.07SYY221 pKa = 8.2LTPYY225 pKa = 9.99LSHH228 pKa = 6.25GRR230 pKa = 11.84YY231 pKa = 8.62FKK233 pKa = 10.56PLNLGQKK240 pKa = 8.84MKK242 pKa = 9.46LTKK245 pKa = 9.88IYY247 pKa = 10.03LKK249 pKa = 10.39KK250 pKa = 10.56FSRR253 pKa = 11.84VLCLAIGFASAFTYY267 pKa = 10.44SYY269 pKa = 9.01ITQPKK274 pKa = 8.51PEE276 pKa = 4.15VKK278 pKa = 10.17KK279 pKa = 11.04VVSQTYY285 pKa = 10.61DD286 pKa = 3.05FDD288 pKa = 4.36KK289 pKa = 10.7FTIDD293 pKa = 3.03SSQRR297 pKa = 11.84LNLSYY302 pKa = 10.63RR303 pKa = 11.84YY304 pKa = 9.78VFKK307 pKa = 10.71DD308 pKa = 3.41SKK310 pKa = 11.38GKK312 pKa = 10.47LINSDD317 pKa = 4.1DD318 pKa = 4.08LQKK321 pKa = 10.71QGYY324 pKa = 8.1SLTYY328 pKa = 9.83IDD330 pKa = 5.34LCTVSIKK337 pKa = 10.46KK338 pKa = 10.48GNSNEE343 pKa = 3.96IVKK346 pKa = 10.79CNN348 pKa = 3.35

Molecular weight: 39.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

2056

32

426

205.6

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.08 ± 0.937

1.216 ± 0.316

5.642 ± 0.398

3.599 ± 0.644

5.447 ± 0.362

7.879 ± 1.623

0.827 ± 0.229

5.593 ± 0.711

5.447 ± 0.872

9.776 ± 1.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.347

5.642 ± 0.852

4.426 ± 0.463

3.94 ± 0.315

3.745 ± 0.496

10.117 ± 0.841

5.545 ± 0.322

7.636 ± 0.799

0.973 ± 0.198

4.037 ± 0.694
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski