Leucobacter sp. UCD-THU

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061LXI6|A0A061LXI6_9MICO Glutamine synthetase OS=Leucobacter sp. UCD-THU OX=1292023 GN=H490_0112870 PE=3 SV=1
MM1 pKa = 7.82SSRR4 pKa = 11.84TVSARR9 pKa = 11.84AALPAILAASLLTLVACSGSGGGKK33 pKa = 9.25NASDD37 pKa = 3.99DD38 pKa = 3.91EE39 pKa = 5.22GPLSEE44 pKa = 4.64YY45 pKa = 10.85LSSLSSAEE53 pKa = 4.14TEE55 pKa = 3.81SEE57 pKa = 3.97EE58 pKa = 4.14FYY60 pKa = 11.4EE61 pKa = 4.57RR62 pKa = 11.84INAKK66 pKa = 10.14RR67 pKa = 11.84EE68 pKa = 3.92EE69 pKa = 4.2LVAEE73 pKa = 4.49CMAGEE78 pKa = 4.39GFDD81 pKa = 3.92YY82 pKa = 10.98QPNSQNGGTSTLGDD96 pKa = 4.63DD97 pKa = 4.45GIEE100 pKa = 3.92WGSLEE105 pKa = 4.08FAEE108 pKa = 4.38QYY110 pKa = 11.16GYY112 pKa = 11.36GIVDD116 pKa = 3.47WPGLAEE122 pKa = 4.22MEE124 pKa = 4.41QQSEE128 pKa = 5.08EE129 pKa = 4.24YY130 pKa = 10.6VDD132 pKa = 4.05PNQGYY137 pKa = 9.1VEE139 pKa = 4.29SLSEE143 pKa = 4.23SEE145 pKa = 3.86QQAYY149 pKa = 10.12YY150 pKa = 10.1EE151 pKa = 4.67ALSGPQPSEE160 pKa = 3.48EE161 pKa = 4.4DD162 pKa = 3.14MAAMEE167 pKa = 4.83DD168 pKa = 5.02GAYY171 pKa = 9.7EE172 pKa = 4.05WDD174 pKa = 3.43WTQSGCYY181 pKa = 9.13GAADD185 pKa = 3.89HH186 pKa = 6.73EE187 pKa = 4.72VQDD190 pKa = 4.41EE191 pKa = 4.52YY192 pKa = 11.55GSAMAAYY199 pKa = 7.87EE200 pKa = 4.12DD201 pKa = 4.41PEE203 pKa = 3.99FAEE206 pKa = 5.16LFEE209 pKa = 4.77SMNAVWSEE217 pKa = 3.83LYY219 pKa = 10.9NYY221 pKa = 9.0DD222 pKa = 4.87DD223 pKa = 5.28PGAPSPNDD231 pKa = 3.37DD232 pKa = 3.35VAKK235 pKa = 10.59LDD237 pKa = 4.2RR238 pKa = 11.84EE239 pKa = 4.21WSEE242 pKa = 4.2CMADD246 pKa = 4.14AGYY249 pKa = 9.08TDD251 pKa = 4.88LVSPQTAMDD260 pKa = 4.13GLNEE264 pKa = 4.07EE265 pKa = 4.73WNTLQTPPEE274 pKa = 4.21GTGDD278 pKa = 4.82DD279 pKa = 3.65LAGWSGPSDD288 pKa = 3.44RR289 pKa = 11.84QKK291 pKa = 11.3SEE293 pKa = 3.65FQQRR297 pKa = 11.84EE298 pKa = 3.5IEE300 pKa = 4.24MAVTDD305 pKa = 4.55WKK307 pKa = 11.17CKK309 pKa = 10.52DD310 pKa = 3.58GIDD313 pKa = 3.9YY314 pKa = 7.68EE315 pKa = 4.48QQVRR319 pKa = 11.84TVQFDD324 pKa = 3.66LEE326 pKa = 4.11QRR328 pKa = 11.84FVDD331 pKa = 3.5EE332 pKa = 5.35HH333 pKa = 6.25RR334 pKa = 11.84AEE336 pKa = 4.35LDD338 pKa = 3.22ALIAKK343 pKa = 9.96HH344 pKa = 6.41GADD347 pKa = 3.29QKK349 pKa = 11.46KK350 pKa = 10.08SS351 pKa = 3.35

Molecular weight: 38.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061LYY7|A0A061LYY7_9MICO Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase OS=Leucobacter sp. UCD-THU OX=1292023 GN=lgt PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2878

0

2878

968228

36

5399

336.4

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.622 ± 0.082

0.545 ± 0.01

5.8 ± 0.045

6.392 ± 0.045

3.126 ± 0.03

9.293 ± 0.041

1.947 ± 0.02

4.538 ± 0.033

1.87 ± 0.034

10.292 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.815 ± 0.017

1.872 ± 0.026

5.476 ± 0.031

2.994 ± 0.026

7.579 ± 0.053

5.578 ± 0.028

5.474 ± 0.038

8.496 ± 0.039

1.409 ± 0.021

1.88 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski