Klebsiella phage 4LV2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Klebsiella virus 4LV2017

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2FB28|A0A2Z2FB28_9CAUD Uncharacterized protein OS=Klebsiella phage 4LV2017 OX=1960658 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84FSVSTQSFYY11 pKa = 11.54DD12 pKa = 3.63DD13 pKa = 4.03NYY15 pKa = 10.54EE16 pKa = 3.9EE17 pKa = 4.16NAIVDD22 pKa = 4.47DD23 pKa = 4.94LPSDD27 pKa = 3.71VQAIDD32 pKa = 3.22NEE34 pKa = 4.38QYY36 pKa = 11.57ARR38 pKa = 11.84FFNAINSDD46 pKa = 3.39RR47 pKa = 11.84VVYY50 pKa = 10.08LVADD54 pKa = 4.94KK55 pKa = 11.56YY56 pKa = 11.24NISQPRR62 pKa = 11.84PDD64 pKa = 5.1KK65 pKa = 10.12YY66 pKa = 10.74HH67 pKa = 6.21SWDD70 pKa = 3.25AAGNTWVMTDD80 pKa = 3.06AAVTRR85 pKa = 11.84KK86 pKa = 9.93SADD89 pKa = 3.73LIADD93 pKa = 3.69AEE95 pKa = 4.36LRR97 pKa = 11.84RR98 pKa = 11.84STLLSEE104 pKa = 4.7AGTAISPLQDD114 pKa = 3.24AVEE117 pKa = 4.65LDD119 pKa = 3.39MATDD123 pKa = 4.05EE124 pKa = 4.38EE125 pKa = 4.78KK126 pKa = 10.8SRR128 pKa = 11.84YY129 pKa = 8.91DD130 pKa = 2.56AWRR133 pKa = 11.84KK134 pKa = 9.36YY135 pKa = 9.71RR136 pKa = 11.84VLLMRR141 pKa = 11.84VDD143 pKa = 3.71TSLAPDD149 pKa = 3.88INWPEE154 pKa = 3.98PPKK157 pKa = 10.8DD158 pKa = 3.32

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2FG11|A0A2Z2FG11_9CAUD Uncharacterized protein OS=Klebsiella phage 4LV2017 OX=1960658 PE=4 SV=1
MM1 pKa = 8.05AEE3 pKa = 4.1LQKK6 pKa = 10.84VDD8 pKa = 3.78DD9 pKa = 4.36WLSALLANLEE19 pKa = 3.93PAARR23 pKa = 11.84SRR25 pKa = 11.84MMRR28 pKa = 11.84QLAQEE33 pKa = 4.22LRR35 pKa = 11.84RR36 pKa = 11.84TQQQNIRR43 pKa = 11.84MQRR46 pKa = 11.84NPDD49 pKa = 3.11GSSYY53 pKa = 10.51EE54 pKa = 3.9PRR56 pKa = 11.84RR57 pKa = 11.84VTARR61 pKa = 11.84SKK63 pKa = 10.21KK64 pKa = 10.29GRR66 pKa = 11.84IKK68 pKa = 10.6RR69 pKa = 11.84QMFTKK74 pKa = 10.55LRR76 pKa = 11.84TTKK79 pKa = 10.31YY80 pKa = 10.39LKK82 pKa = 9.46TAASADD88 pKa = 3.74SASVQFEE95 pKa = 4.16GKK97 pKa = 6.93VQRR100 pKa = 11.84IARR103 pKa = 11.84VHH105 pKa = 6.27HH106 pKa = 5.92YY107 pKa = 10.35GLRR110 pKa = 11.84DD111 pKa = 3.15RR112 pKa = 11.84VSRR115 pKa = 11.84KK116 pKa = 9.9GPEE119 pKa = 3.28VRR121 pKa = 11.84YY122 pKa = 10.33AEE124 pKa = 4.04RR125 pKa = 11.84RR126 pKa = 11.84LLGVNDD132 pKa = 4.35DD133 pKa = 4.08VEE135 pKa = 5.45AMTRR139 pKa = 11.84DD140 pKa = 5.0IILQWLAGG148 pKa = 3.74

Molecular weight: 17.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

9591

38

1025

252.4

28.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.801 ± 0.95

1.001 ± 0.173

5.411 ± 0.335

6.152 ± 0.347

3.608 ± 0.324

6.277 ± 0.424

1.814 ± 0.247

4.953 ± 0.392

5.495 ± 0.346

9.999 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.167

4.463 ± 0.227

4.066 ± 0.164

4.087 ± 0.23

6.235 ± 0.431

6.652 ± 0.264

6.12 ± 0.429

6.61 ± 0.353

1.554 ± 0.133

2.951 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski