Coraliomargarita sp. CAG:312

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Coraliomargarita; environmental samples

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R7L6V1|R7L6V1_9BACT UPF0365 protein BN601_00101 OS=Coraliomargarita sp. CAG:312 OX=1262865 GN=BN601_00101 PE=3 SV=1
MM1 pKa = 7.58TKK3 pKa = 10.49NKK5 pKa = 10.18DD6 pKa = 3.48DD7 pKa = 4.8LGFLSIMADD16 pKa = 3.08KK17 pKa = 11.23NEE19 pKa = 4.0KK20 pKa = 10.73NEE22 pKa = 3.89FNVPGKK28 pKa = 10.45FYY30 pKa = 11.21VDD32 pKa = 3.76SQCIGCALCASTAEE46 pKa = 4.17GLFAISDD53 pKa = 3.65TGCAYY58 pKa = 10.12VAKK61 pKa = 10.37QPASDD66 pKa = 4.68EE67 pKa = 4.13EE68 pKa = 4.44TALCTEE74 pKa = 4.36AMEE77 pKa = 4.56SCPVQAIGDD86 pKa = 3.97DD87 pKa = 4.36GEE89 pKa = 4.11

Molecular weight:
9.42 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R7LEZ6|R7LEZ6_9BACT ATP-binding protein Uup OS=Coraliomargarita sp. CAG:312 OX=1262865 GN=uup PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84RR3 pKa = 11.84LNGVQSVPLYY13 pKa = 11.0FNMLRR18 pKa = 11.84GNFFFIQRR26 pKa = 11.84ILPNNSHH33 pKa = 5.36AWKK36 pKa = 9.93RR37 pKa = 11.84ANSYY41 pKa = 9.97KK42 pKa = 10.62VNNAKK47 pKa = 7.29TQKK50 pKa = 9.77NARR53 pKa = 11.84LRR55 pKa = 11.84ICLHH59 pKa = 6.26FAPILPHH66 pKa = 5.51GACRR70 pKa = 11.84EE71 pKa = 4.12SFRR74 pKa = 11.84ACGDD78 pKa = 3.03WKK80 pKa = 10.7FRR82 pKa = 11.84ARR84 pKa = 11.84QAYY87 pKa = 9.39AKK89 pKa = 10.42AHH91 pKa = 6.39SNYY94 pKa = 8.66FWQVFPALNSGLDD107 pKa = 3.5VSWQRR112 pKa = 11.84EE113 pKa = 3.99QEE115 pKa = 4.12SAAEE119 pKa = 4.06FEE121 pKa = 4.64CGSWHH126 pKa = 4.9TTQLNPLWSPVGVRR140 pKa = 11.84SSQEE144 pKa = 3.83RR145 pKa = 11.84AWRR148 pKa = 11.84SQLGEE153 pKa = 3.88

Molecular weight:
17.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2060

0

2060

677430

29

3629

328.8

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.101 ± 0.068

1.546 ± 0.021

5.563 ± 0.042

6.175 ± 0.043

4.988 ± 0.037

7.323 ± 0.044

1.449 ± 0.019

6.872 ± 0.047

7.049 ± 0.058

8.581 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.025

4.534 ± 0.048

4.123 ± 0.034

2.548 ± 0.029

4.914 ± 0.039

7.422 ± 0.053

4.421 ± 0.047

6.261 ± 0.044

1.178 ± 0.021

3.311 ± 0.041

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski