Achromatium sp. WMS3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Achromatium; unclassified Achromatium

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M1JBI1|A0A0M1JBI1_9GAMM Glutamine-synthetase adenylyltransferase (Fragment) OS=Achromatium sp. WMS3 OX=1604836 GN=TI05_13615 PE=4 SV=1
MM1 pKa = 7.63LALDD5 pKa = 3.36QTGPYY10 pKa = 9.53LFQVRR15 pKa = 11.84HH16 pKa = 5.3GVALATGQDD25 pKa = 3.6DD26 pKa = 4.98VIDD29 pKa = 4.31GGAGNDD35 pKa = 3.82TLIGGPGNDD44 pKa = 4.03TIAGGSGWDD53 pKa = 3.33TAVYY57 pKa = 9.81SGNYY61 pKa = 9.49DD62 pKa = 3.35EE63 pKa = 5.01YY64 pKa = 11.25QITEE68 pKa = 4.24TDD70 pKa = 3.39TGWTVKK76 pKa = 10.6DD77 pKa = 3.8NRR79 pKa = 11.84QDD81 pKa = 3.71SPDD84 pKa = 3.62GTDD87 pKa = 3.4TISDD91 pKa = 3.57DD92 pKa = 3.98VEE94 pKa = 4.13NLEE97 pKa = 5.1FADD100 pKa = 3.67QTVSLVPTAEE110 pKa = 4.21EE111 pKa = 3.72IFIAEE116 pKa = 4.32GNDD119 pKa = 3.4GLRR122 pKa = 11.84NNEE125 pKa = 4.03AYY127 pKa = 7.96FTAGMMRR134 pKa = 11.84VMADD138 pKa = 3.31FMKK141 pKa = 10.64ASYY144 pKa = 10.61SLEE147 pKa = 3.77EE148 pKa = 4.18SEE150 pKa = 4.71NRR152 pKa = 11.84VINDD156 pKa = 3.38EE157 pKa = 4.19NGGADD162 pKa = 3.25TALTEE167 pKa = 5.51LYY169 pKa = 10.47DD170 pKa = 4.15QGWQPVFDD178 pKa = 5.33LIIPGVEE185 pKa = 3.51ATTILTVEE193 pKa = 4.95DD194 pKa = 3.77LTASYY199 pKa = 10.74QEE201 pKa = 4.11EE202 pKa = 4.22DD203 pKa = 3.66AEE205 pKa = 4.34QSGLASQMVSNGFSQEE221 pKa = 3.62TGLFTNGNAAALILRR236 pKa = 11.84SGDD239 pKa = 3.66SLVLSFRR246 pKa = 11.84GTNDD250 pKa = 2.89TGSPNDD256 pKa = 4.06DD257 pKa = 3.35SGILNTSDD265 pKa = 4.06PNNIIYY271 pKa = 9.46PDD273 pKa = 4.34RR274 pKa = 11.84IHH276 pKa = 6.87WGGAASIFDD285 pKa = 3.8SWSEE289 pKa = 3.94WEE291 pKa = 4.3GRR293 pKa = 11.84DD294 pKa = 4.13ANMSDD299 pKa = 3.97HH300 pKa = 6.48YY301 pKa = 11.84ALFAPLITAIEE312 pKa = 4.31NYY314 pKa = 9.89VGNADD319 pKa = 3.3NGINHH324 pKa = 6.12VYY326 pKa = 9.13ATGHH330 pKa = 5.61SLGGAMAIEE339 pKa = 4.68FTSQHH344 pKa = 6.03EE345 pKa = 4.38DD346 pKa = 3.2MVDD349 pKa = 3.3QTITFAAPAFTNEE362 pKa = 3.7NLSRR366 pKa = 11.84EE367 pKa = 4.16EE368 pKa = 5.79FEE370 pKa = 6.0DD371 pKa = 4.84LDD373 pKa = 3.88NLTQVEE379 pKa = 4.42ILFDD383 pKa = 4.47PVPMTWDD390 pKa = 2.96SLGGVNRR397 pKa = 11.84PGQLIRR403 pKa = 11.84LAGDD407 pKa = 3.61GTSTPIDD414 pKa = 3.58EE415 pKa = 4.85NFDD418 pKa = 4.19NILGADD424 pKa = 3.82SVNSNHH430 pKa = 5.17STNYY434 pKa = 9.76YY435 pKa = 9.03RR436 pKa = 11.84TITDD440 pKa = 4.92AIDD443 pKa = 3.52SVSWQAIINGTDD455 pKa = 3.06AQDD458 pKa = 3.14IYY460 pKa = 11.01LDD462 pKa = 3.54GMRR465 pKa = 11.84EE466 pKa = 3.93VEE468 pKa = 3.98NRR470 pKa = 11.84EE471 pKa = 3.65EE472 pKa = 4.32SFVVSVNDD480 pKa = 4.74DD481 pKa = 3.86VITYY485 pKa = 9.78DD486 pKa = 4.08PNWNDD491 pKa = 2.99FFDD494 pKa = 5.4GYY496 pKa = 11.06FGDD499 pKa = 4.04EE500 pKa = 4.47FGFDD504 pKa = 3.42AYY506 pKa = 11.38YY507 pKa = 10.89GGNGNDD513 pKa = 3.59HH514 pKa = 6.22LTGGEE519 pKa = 3.99EE520 pKa = 4.16GDD522 pKa = 3.62ILFGGGDD529 pKa = 3.79NDD531 pKa = 3.59RR532 pKa = 11.84LAGRR536 pKa = 11.84GGDD539 pKa = 3.72DD540 pKa = 2.82IFYY543 pKa = 10.58GGSGNDD549 pKa = 3.65RR550 pKa = 11.84IHH552 pKa = 7.29GGEE555 pKa = 4.03GTDD558 pKa = 3.29TVVYY562 pKa = 10.5ASSADD567 pKa = 3.57NYY569 pKa = 11.46DD570 pKa = 3.55FAIEE574 pKa = 3.86NWALTVKK581 pKa = 10.36PHH583 pKa = 6.64DD584 pKa = 3.98TNEE587 pKa = 3.76TDD589 pKa = 2.98TLYY592 pKa = 10.95SIEE595 pKa = 3.83QLGFGGEE602 pKa = 4.25QPVSVASEE610 pKa = 3.71LSYY613 pKa = 11.48TNNQLLHH620 pKa = 6.4MYY622 pKa = 10.74APILAMNADD631 pKa = 4.42DD632 pKa = 4.24YY633 pKa = 11.79VPTRR637 pKa = 11.84IEE639 pKa = 3.85AFLDD643 pKa = 3.35HH644 pKa = 7.44AILFDD649 pKa = 4.17VVWGNNPLAVGNNISGIVYY668 pKa = 10.33EE669 pKa = 5.19EE670 pKa = 4.15GVQYY674 pKa = 10.92NINTFVTGTSSIEE687 pKa = 3.88NAEE690 pKa = 4.14LVDD693 pKa = 4.5DD694 pKa = 4.4VSNLLSEE701 pKa = 4.66EE702 pKa = 4.15FHH704 pKa = 6.51SHH706 pKa = 8.01LIRR709 pKa = 11.84SNDD712 pKa = 3.25YY713 pKa = 10.3YY714 pKa = 11.64LDD716 pKa = 4.88FISGTEE722 pKa = 3.92NSATRR727 pKa = 11.84VDD729 pKa = 4.05PQTEE733 pKa = 4.33VPKK736 pKa = 10.8SYY738 pKa = 11.14GRR740 pKa = 11.84MDD742 pKa = 4.01TNPGSTGSGEE752 pKa = 3.93WADD755 pKa = 3.65SNEE758 pKa = 4.16NQFSFDD764 pKa = 3.38PTVIDD769 pKa = 3.88DD770 pKa = 3.61QYY772 pKa = 10.74GASVYY777 pKa = 10.57GRR779 pKa = 11.84VVTPEE784 pKa = 4.22AGDD787 pKa = 4.25AIYY790 pKa = 10.08LQYY793 pKa = 11.4YY794 pKa = 8.41FFYY797 pKa = 11.07LEE799 pKa = 4.18NHH801 pKa = 4.83WTSPISRR808 pKa = 11.84GFHH811 pKa = 4.81EE812 pKa = 5.25ADD814 pKa = 2.99WEE816 pKa = 4.39FMQIEE821 pKa = 4.47LDD823 pKa = 4.04KK824 pKa = 11.13DD825 pKa = 3.63TLLPDD830 pKa = 3.61TFMSSIHH837 pKa = 7.05LDD839 pKa = 3.19HH840 pKa = 6.74SQNKK844 pKa = 7.86PQYY847 pKa = 8.91PNQIHH852 pKa = 4.45QQSYY856 pKa = 9.43RR857 pKa = 11.84FQGLQSVYY865 pKa = 9.99HH866 pKa = 5.87YY867 pKa = 9.19PHH869 pKa = 6.8HH870 pKa = 6.8HH871 pKa = 5.81QLHH874 pKa = 6.29HH875 pKa = 6.68PVLL878 pKa = 4.79

Molecular weight: 96.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M1JJG4|A0A0M1JJG4_9GAMM Uncharacterized protein OS=Achromatium sp. WMS3 OX=1604836 GN=TI05_01120 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.12RR3 pKa = 11.84YY4 pKa = 9.47IHH6 pKa = 6.89LLVAWLFIITAQQTYY21 pKa = 9.6AADD24 pKa = 3.62VGGSIPNTTEE34 pKa = 3.17ATHH37 pKa = 6.75AVTTRR42 pKa = 11.84KK43 pKa = 9.3IVLDD47 pKa = 3.64PCIKK51 pKa = 9.01TRR53 pKa = 11.84KK54 pKa = 9.04IVFNGSKK61 pKa = 9.05TPCRR65 pKa = 11.84RR66 pKa = 11.84YY67 pKa = 10.11KK68 pKa = 10.49RR69 pKa = 11.84PKK71 pKa = 7.74TSSRR75 pKa = 11.84PSCYY79 pKa = 10.13ARR81 pKa = 11.84GPRR84 pKa = 11.84TRR86 pKa = 11.84KK87 pKa = 9.38IVWGKK92 pKa = 8.89EE93 pKa = 3.65YY94 pKa = 10.92CRR96 pKa = 4.24

Molecular weight: 11.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3099

0

3099

775224

26

3350

250.2

28.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.061 ± 0.054

1.075 ± 0.022

5.139 ± 0.053

5.471 ± 0.046

3.904 ± 0.036

6.194 ± 0.048

2.395 ± 0.024

7.909 ± 0.045

5.584 ± 0.047

10.687 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.024

4.863 ± 0.042

4.377 ± 0.032

4.994 ± 0.046

4.887 ± 0.042

5.65 ± 0.04

6.181 ± 0.06

5.8 ± 0.043

1.359 ± 0.02

3.324 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski