Geobacillus phage GBK2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8ECW0|W8ECW0_9CAUD Holin OS=Geobacillus phage GBK2 OX=1458842 GN=GBK2_23 PE=4 SV=1
MM1 pKa = 7.65INFEE5 pKa = 5.29LFTQLIEE12 pKa = 3.86VDD14 pKa = 3.34MSKK17 pKa = 10.56KK18 pKa = 9.95VDD20 pKa = 4.13EE21 pKa = 4.83IEE23 pKa = 4.71IDD25 pKa = 3.66LQIYY29 pKa = 10.17DD30 pKa = 3.83CTGEE34 pKa = 4.19VNITDD39 pKa = 3.4IMLQGGSIATVWTGHH54 pKa = 6.03PSEE57 pKa = 5.22LRR59 pKa = 11.84WSHH62 pKa = 7.35DD63 pKa = 3.51YY64 pKa = 11.33

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8ECX3|W8ECX3_9CAUD Recombinase OS=Geobacillus phage GBK2 OX=1458842 GN=GBK2_38 PE=4 SV=1
MM1 pKa = 7.45LNARR5 pKa = 11.84AKK7 pKa = 9.52INKK10 pKa = 8.46RR11 pKa = 11.84RR12 pKa = 11.84AGLRR16 pKa = 11.84ILLLMRR22 pKa = 11.84VFGKK26 pKa = 10.26IFSVFSAIFFDD37 pKa = 3.74LGVYY41 pKa = 9.31KK42 pKa = 10.73VNKK45 pKa = 9.37IVYY48 pKa = 9.22NKK50 pKa = 10.57

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12314

44

988

198.6

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.634 ± 0.447

0.958 ± 0.161

5.392 ± 0.311

7.902 ± 0.45

4.036 ± 0.238

6.797 ± 0.258

1.559 ± 0.151

7.171 ± 0.271

8.226 ± 0.488

7.78 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.102 ± 0.246

5.66 ± 0.241

3.987 ± 0.215

3.898 ± 0.218

5.051 ± 0.257

3.874 ± 0.232

5.222 ± 0.355

5.944 ± 0.217

1.6 ± 0.111

4.207 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski