Sphingomonas sp. WZY 27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369VZ37|A0A369VZ37_9SPHN Permease OS=Sphingomonas sp. WZY 27 OX=2283317 GN=DVW87_05275 PE=4 SV=1
MM1 pKa = 7.82ALPNYY6 pKa = 10.43DD7 pKa = 3.53FLQYY11 pKa = 11.04ILTTSDD17 pKa = 2.85IVIQDD22 pKa = 3.43FVRR25 pKa = 11.84RR26 pKa = 11.84LCMAAISDD34 pKa = 3.76DD35 pKa = 3.73DD36 pKa = 4.12AAVFGWSTVLQLVKK50 pKa = 10.84AGWVPAADD58 pKa = 4.32LPSNDD63 pKa = 3.41IACVIANLCLAPTQTDD79 pKa = 3.57DD80 pKa = 5.69ADD82 pKa = 3.75MANCPDD88 pKa = 3.93RR89 pKa = 11.84GDD91 pKa = 3.59GRR93 pKa = 11.84LIPHH97 pKa = 7.4

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369W4Z8|A0A369W4Z8_9SPHN Spore coat U domain-containing protein OS=Sphingomonas sp. WZY 27 OX=2283317 GN=DVW87_05655 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3410

0

3410

1085316

28

3492

318.3

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.228 ± 0.058

0.733 ± 0.011

5.745 ± 0.03

5.341 ± 0.039

3.411 ± 0.026

9.067 ± 0.042

1.915 ± 0.022

4.338 ± 0.027

2.567 ± 0.035

10.129 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.022

2.386 ± 0.029

5.534 ± 0.037

3.146 ± 0.023

7.929 ± 0.051

4.945 ± 0.034

5.435 ± 0.039

7.455 ± 0.034

1.446 ± 0.018

2.094 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski