Bhargavaea cecembensis DSE10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Bhargavaea; Bhargavaea cecembensis

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7NDL7|M7NDL7_9BACL Rhodanese domain-containing protein OS=Bhargavaea cecembensis DSE10 OX=1235279 GN=C772_01191 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84KK3 pKa = 9.69GLLVTLVALLCGAILIFSYY22 pKa = 9.38LTYY25 pKa = 10.62QNRR28 pKa = 11.84LEE30 pKa = 3.82EE31 pKa = 4.17AARR34 pKa = 11.84VKK36 pKa = 10.04TDD38 pKa = 3.09EE39 pKa = 4.18EE40 pKa = 5.14TVTNEE45 pKa = 3.82PEE47 pKa = 4.07EE48 pKa = 4.36NTTSEE53 pKa = 4.22PEE55 pKa = 3.94QNGEE59 pKa = 4.16EE60 pKa = 4.24NTDD63 pKa = 3.5SVSIKK68 pKa = 9.95QEE70 pKa = 3.73EE71 pKa = 4.37LEE73 pKa = 4.11ILSANTDD80 pKa = 3.06AKK82 pKa = 10.07VQEE85 pKa = 4.23VLLARR90 pKa = 11.84LEE92 pKa = 4.43AGEE95 pKa = 4.07PAQMVVIGSGEE106 pKa = 3.79IQGIADD112 pKa = 4.36RR113 pKa = 11.84LADD116 pKa = 4.26AVNEE120 pKa = 4.19AYY122 pKa = 10.52GDD124 pKa = 3.97FLTVDD129 pKa = 3.85AFAFDD134 pKa = 3.59GTSAEE139 pKa = 4.32FVEE142 pKa = 4.73QGVPNLAWTNEE153 pKa = 3.83YY154 pKa = 10.87DD155 pKa = 3.31IVLYY159 pKa = 10.7EE160 pKa = 4.76PFTLNNNGIVVIEE173 pKa = 4.93DD174 pKa = 3.32EE175 pKa = 4.61HH176 pKa = 8.03AHH178 pKa = 5.75IAQVEE183 pKa = 4.29EE184 pKa = 4.16RR185 pKa = 11.84AMEE188 pKa = 4.22AVQDD192 pKa = 3.99SAFLITPSHH201 pKa = 6.84PIEE204 pKa = 4.14NAGYY208 pKa = 9.81YY209 pKa = 7.97LTQIDD214 pKa = 4.01SLKK217 pKa = 11.03NYY219 pKa = 10.25AEE221 pKa = 4.18QQGIPYY227 pKa = 9.92VDD229 pKa = 3.57TWNNWPMDD237 pKa = 4.04EE238 pKa = 4.51LDD240 pKa = 4.83SYY242 pKa = 11.58LDD244 pKa = 3.69EE245 pKa = 5.53NGVPTEE251 pKa = 3.88QGLDD255 pKa = 3.17VWADD259 pKa = 3.26ALIQYY264 pKa = 8.1FIAQQ268 pKa = 3.44

Molecular weight: 29.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7NCQ3|M7NCQ3_9BACL Spore cortex biosynthesis protein YabQ OS=Bhargavaea cecembensis DSE10 OX=1235279 GN=C772_02969 PE=4 SV=1
MM1 pKa = 6.76NQRR4 pKa = 11.84RR5 pKa = 11.84LIEE8 pKa = 4.12EE9 pKa = 3.58LWGRR13 pKa = 11.84EE14 pKa = 4.02KK15 pKa = 11.02YY16 pKa = 10.06RR17 pKa = 11.84VMYY20 pKa = 10.2HH21 pKa = 6.23SRR23 pKa = 11.84AHH25 pKa = 7.28DD26 pKa = 3.42DD27 pKa = 4.23RR28 pKa = 11.84IRR30 pKa = 11.84KK31 pKa = 6.45TLKK34 pKa = 10.35ADD36 pKa = 4.02PSPADD41 pKa = 2.98IEE43 pKa = 4.43TRR45 pKa = 11.84SGKK48 pKa = 10.15RR49 pKa = 11.84SGSRR53 pKa = 11.84PTPVRR58 pKa = 11.84QNFCFWSASS67 pKa = 3.39

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3168

0

3168

941780

30

1916

297.3

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.866 ± 0.045

0.584 ± 0.014

5.577 ± 0.044

7.864 ± 0.056

4.301 ± 0.035

8.297 ± 0.039

2.031 ± 0.023

6.618 ± 0.04

5.1 ± 0.038

9.619 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.021

3.241 ± 0.026

4.214 ± 0.026

3.051 ± 0.026

5.468 ± 0.046

5.545 ± 0.025

5.359 ± 0.029

7.282 ± 0.037

1.03 ± 0.017

3.048 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski