Sphaerospermopsis kisseleviana NIES-73

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Aphanizomenonaceae; Sphaerospermopsis; Sphaerospermopsis kisseleviana

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4UVJ0|A0A1Z4UVJ0_9CYAN Putative Na+-transporting ATP synthase OS=Sphaerospermopsis kisseleviana NIES-73 OX=1973480 GN=NIES73_45530 PE=4 SV=1
MM1 pKa = 7.69LLTSTDD7 pKa = 3.06AGKK10 pKa = 10.17IALEE14 pKa = 3.96FLLADD19 pKa = 3.35WNIAEE24 pKa = 5.05EE25 pKa = 4.12YY26 pKa = 10.12RR27 pKa = 11.84DD28 pKa = 3.31WFTIINSQLMGEE40 pKa = 3.97YY41 pKa = 9.52WYY43 pKa = 10.02IVEE46 pKa = 4.77LGVEE50 pKa = 4.62GFPDD54 pKa = 2.69KK55 pKa = 10.86WFIQVYY61 pKa = 8.43DD62 pKa = 4.23TGACDD67 pKa = 3.46PNYY70 pKa = 10.3TFMSPISGSEE80 pKa = 3.98GYY82 pKa = 9.24MDD84 pKa = 6.0LKK86 pKa = 10.93NLPNILADD94 pKa = 3.51VLVAEE99 pKa = 4.73RR100 pKa = 11.84NSRR103 pKa = 3.43

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4UPM2|A0A1Z4UPM2_9CYAN NAD(P) transhydrogenase subunit beta OS=Sphaerospermopsis kisseleviana NIES-73 OX=1973480 GN=NIES73_24440 PE=3 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MQSPTGRR28 pKa = 11.84RR29 pKa = 11.84VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.08KK37 pKa = 9.6GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5038

0

5038

1454114

29

4109

288.6

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.382 ± 0.042

0.997 ± 0.013

4.884 ± 0.023

6.599 ± 0.037

4.006 ± 0.024

6.34 ± 0.034

1.844 ± 0.019

7.394 ± 0.03

5.575 ± 0.035

10.801 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.017

4.903 ± 0.031

4.603 ± 0.026

5.303 ± 0.033

4.682 ± 0.028

6.13 ± 0.03

5.691 ± 0.028

6.384 ± 0.032

1.414 ± 0.016

3.187 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski