Marinisporobacter balticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Marinisporobacter

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2KKW2|A0A4R2KKW2_9CLOT Septal ring factor EnvC (AmiA/AmiB activator) OS=Marinisporobacter balticus OX=2018667 GN=EV214_11629 PE=4 SV=1
MM1 pKa = 7.78PNNIVFNNVARR12 pKa = 11.84QLKK15 pKa = 8.26TEE17 pKa = 3.96IYY19 pKa = 10.22GNDD22 pKa = 3.35GTTTRR27 pKa = 11.84VVAVDD32 pKa = 3.45SSGKK36 pKa = 9.83ILIGNLDD43 pKa = 4.13TINTISYY50 pKa = 9.14IATVDD55 pKa = 3.42TVNYY59 pKa = 8.53VAAVDD64 pKa = 3.87TVDD67 pKa = 3.46YY68 pKa = 10.42VAAVDD73 pKa = 4.17TVDD76 pKa = 3.63EE77 pKa = 4.42VANVSSVDD85 pKa = 3.62TVDD88 pKa = 3.49EE89 pKa = 4.37VVNVSSVDD97 pKa = 3.58TVDD100 pKa = 3.49EE101 pKa = 4.37VVNVSSVDD109 pKa = 3.58TVDD112 pKa = 3.49EE113 pKa = 4.37VVNVSSVDD121 pKa = 3.46TVDD124 pKa = 3.33YY125 pKa = 10.56VAAVDD130 pKa = 4.17TVDD133 pKa = 3.63EE134 pKa = 4.42VANVSSVDD142 pKa = 3.67TVDD145 pKa = 3.73EE146 pKa = 4.31VANVSSVDD154 pKa = 3.41TVDD157 pKa = 3.78YY158 pKa = 10.04VASVHH163 pKa = 5.36TVTHH167 pKa = 5.1VTDD170 pKa = 3.6TVDD173 pKa = 3.5VKK175 pKa = 10.78IVSNDD180 pKa = 3.31YY181 pKa = 9.51TEE183 pKa = 4.45SSSVVVLAGDD193 pKa = 3.93ASAAVLSMDD202 pKa = 3.24TSEE205 pKa = 4.73KK206 pKa = 11.05NMYY209 pKa = 9.73SYY211 pKa = 11.51YY212 pKa = 9.92IASSGAATVIVQISPTTTNSYY233 pKa = 10.77FIDD236 pKa = 4.58DD237 pKa = 3.72ATSAVMTANSTTVLVPEE254 pKa = 4.96RR255 pKa = 11.84FLKK258 pKa = 8.91YY259 pKa = 8.89TRR261 pKa = 11.84LYY263 pKa = 9.16LTAGSGGATASVYY276 pKa = 10.82YY277 pKa = 10.29NSHH280 pKa = 5.19NN281 pKa = 3.45

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2KR54|A0A4R2KR54_9CLOT Dihydrolipoyl dehydrogenase OS=Marinisporobacter balticus OX=2018667 GN=EV214_10957 PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.14KK4 pKa = 10.1SLKK7 pKa = 9.8VKK9 pKa = 10.03QSRR12 pKa = 11.84TQKK15 pKa = 10.4YY16 pKa = 6.9STRR19 pKa = 11.84EE20 pKa = 3.73YY21 pKa = 10.59NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.75AYY33 pKa = 9.43LRR35 pKa = 11.84KK36 pKa = 9.69FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.81LAYY49 pKa = 10.0KK50 pKa = 10.86GEE52 pKa = 4.16IPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3681

0

3681

1099301

26

2848

298.6

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.084 ± 0.043

1.121 ± 0.019

5.376 ± 0.032

7.545 ± 0.049

4.263 ± 0.035

6.634 ± 0.047

1.756 ± 0.019

9.835 ± 0.057

9.031 ± 0.043

8.865 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.022

5.424 ± 0.037

2.979 ± 0.023

2.865 ± 0.024

3.559 ± 0.027

5.511 ± 0.033

5.002 ± 0.03

6.568 ± 0.038

0.724 ± 0.014

3.837 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski