Pneumococcus phage Dp-1 (Bacteriophage Dp-1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7DN88|E7DN88_BPDP1 RNA polymerase sigma 28 subunit OS=Pneumococcus phage Dp-1 OX=59241 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.86RR3 pKa = 11.84IKK5 pKa = 10.12TLFHH9 pKa = 6.4VIYY12 pKa = 10.85ANGTHH17 pKa = 6.69LEE19 pKa = 4.13VAALFDD25 pKa = 4.17TVDD28 pKa = 4.44DD29 pKa = 4.24YY30 pKa = 12.23DD31 pKa = 5.62DD32 pKa = 4.62VIEE35 pKa = 5.62DD36 pKa = 3.32IQGYY40 pKa = 9.98IDD42 pKa = 4.8TPDD45 pKa = 4.17LYY47 pKa = 10.14NQRR50 pKa = 11.84SIRR53 pKa = 11.84MAPYY57 pKa = 10.39NPDD60 pKa = 3.14INGDD64 pKa = 4.32AIATDD69 pKa = 3.11ILLRR73 pKa = 11.84LDD75 pKa = 4.43DD76 pKa = 5.23IIYY79 pKa = 10.74VDD81 pKa = 4.3ATCEE85 pKa = 3.95TIKK88 pKa = 10.94YY89 pKa = 8.51EE90 pKa = 4.21EE91 pKa = 5.37PIAA94 pKa = 5.0

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O03977|O03977_BPDP1 Uncharacterized protein OS=Pneumococcus phage Dp-1 OX=59241 GN=orf5 PE=4 SV=1
MM1 pKa = 7.2VKK3 pKa = 10.24KK4 pKa = 10.12LKK6 pKa = 10.84SKK8 pKa = 9.91IYY10 pKa = 8.89SVAYY14 pKa = 9.4IILVVIANLVTIYY27 pKa = 10.45FEE29 pKa = 4.1PLNVKK34 pKa = 10.35GILIPPSSWFMGFTFLLINLISKK57 pKa = 8.76YY58 pKa = 9.82EE59 pKa = 3.92KK60 pKa = 10.3PKK62 pKa = 10.66FAGSLIWVGLFLTSLICFMQNLPQSLVVASGVAFWISQKK101 pKa = 11.01ASVFIFDD108 pKa = 4.24KK109 pKa = 11.02LSNKK113 pKa = 9.71LDD115 pKa = 3.78SKK117 pKa = 10.6IANALSSNIGSIIDD131 pKa = 3.43ATIWISLGLSPLGIGTVAYY150 pKa = 9.63IDD152 pKa = 3.81IPSAVLGQVLVQFILQSIASRR173 pKa = 11.84YY174 pKa = 8.62LKK176 pKa = 10.58KK177 pKa = 10.85

Molecular weight: 19.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

17684

47

1230

245.6

27.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.142 ± 0.471

0.927 ± 0.12

5.938 ± 0.281

7.866 ± 0.398

4.156 ± 0.169

6.418 ± 0.367

1.504 ± 0.124

6.854 ± 0.274

7.266 ± 0.342

8.414 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.165

4.925 ± 0.25

3.252 ± 0.159

4.111 ± 0.226

4.552 ± 0.212

6.492 ± 0.28

5.728 ± 0.21

6.514 ± 0.204

1.284 ± 0.129

3.975 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski