Asterias forbesi associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus; unclassified Circovirus

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4FV52|A0A0H4FV52_9CIRC Putative capsid protein OS=Asterias forbesi associated circular virus OX=1676988 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 10.16SRR4 pKa = 11.84NWIFTINNEE13 pKa = 4.84LIWKK17 pKa = 8.84PEE19 pKa = 3.32NHH21 pKa = 6.89LIKK24 pKa = 10.59FVVYY28 pKa = 10.11QLEE31 pKa = 4.14EE32 pKa = 4.34GEE34 pKa = 4.44KK35 pKa = 10.37EE36 pKa = 4.23KK37 pKa = 11.18VPHH40 pKa = 5.29IQGYY44 pKa = 10.18LEE46 pKa = 4.46LKK48 pKa = 9.95QPRR51 pKa = 11.84ALSWMKK57 pKa = 10.66KK58 pKa = 9.87LNSKK62 pKa = 9.41AHH64 pKa = 5.01WEE66 pKa = 3.78IRR68 pKa = 11.84KK69 pKa = 9.7GNRR72 pKa = 11.84VQALQYY78 pKa = 7.97VTKK81 pKa = 10.59EE82 pKa = 4.09EE83 pKa = 4.14TRR85 pKa = 11.84MDD87 pKa = 3.87APWILNCGQWSQYY100 pKa = 10.43DD101 pKa = 3.28ASTWTRR107 pKa = 11.84VEE109 pKa = 3.89DD110 pKa = 3.56WLNTVIPGVWKK121 pKa = 10.65DD122 pKa = 3.6GSKK125 pKa = 8.59STKK128 pKa = 10.02SIRR131 pKa = 11.84LSEE134 pKa = 4.26IKK136 pKa = 10.6SKK138 pKa = 11.1LDD140 pKa = 3.81DD141 pKa = 4.29GCSSEE146 pKa = 5.43VIADD150 pKa = 3.62NYY152 pKa = 9.98FEE154 pKa = 4.8DD155 pKa = 3.62WVRR158 pKa = 11.84HH159 pKa = 3.64YY160 pKa = 10.89RR161 pKa = 11.84AFEE164 pKa = 3.98KK165 pKa = 11.09YY166 pKa = 10.52LLIKK170 pKa = 10.31SKK172 pKa = 10.44PRR174 pKa = 11.84DD175 pKa = 3.4YY176 pKa = 10.38PVRR179 pKa = 11.84VVVVIGPTGTGKK191 pKa = 10.38SRR193 pKa = 11.84FCMEE197 pKa = 4.71QYY199 pKa = 10.85PDD201 pKa = 4.6AYY203 pKa = 9.31WKK205 pKa = 10.09QRR207 pKa = 11.84SQWWDD212 pKa = 3.42GYY214 pKa = 9.88VRR216 pKa = 11.84HH217 pKa = 5.82KK218 pKa = 9.83TIILDD223 pKa = 4.02EE224 pKa = 4.48YY225 pKa = 10.88YY226 pKa = 11.01GWLPFDD232 pKa = 4.48LLLRR236 pKa = 11.84LCDD239 pKa = 4.37RR240 pKa = 11.84YY241 pKa = 10.68PLMLEE246 pKa = 4.36TKK248 pKa = 10.34GGQCNCEE255 pKa = 3.87ANTIVITSNNRR266 pKa = 11.84PDD268 pKa = 3.19SWYY271 pKa = 10.39KK272 pKa = 10.1NCYY275 pKa = 8.81FPAFEE280 pKa = 4.34RR281 pKa = 11.84RR282 pKa = 11.84VDD284 pKa = 3.18TFIIMKK290 pKa = 9.59EE291 pKa = 3.98LGSSFIFKK299 pKa = 9.88KK300 pKa = 9.94WSDD303 pKa = 3.79AEE305 pKa = 4.17KK306 pKa = 9.33HH307 pKa = 5.35TDD309 pKa = 3.61PLILNN314 pKa = 4.52

Molecular weight: 37.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4FV52|A0A0H4FV52_9CIRC Putative capsid protein OS=Asterias forbesi associated circular virus OX=1676988 PE=4 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84NTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84TGRR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84TLRR19 pKa = 11.84RR20 pKa = 11.84GYY22 pKa = 10.67RR23 pKa = 11.84KK24 pKa = 8.34FTGGRR29 pKa = 11.84GITKK33 pKa = 9.77EE34 pKa = 3.6HH35 pKa = 7.21DD36 pKa = 3.22RR37 pKa = 11.84TNIYY41 pKa = 9.25YY42 pKa = 10.13KK43 pKa = 10.7KK44 pKa = 10.37RR45 pKa = 11.84MPRR48 pKa = 11.84FKK50 pKa = 10.23KK51 pKa = 9.92RR52 pKa = 11.84RR53 pKa = 11.84WRR55 pKa = 11.84RR56 pKa = 11.84FSKK59 pKa = 10.3KK60 pKa = 8.4VQNVSEE66 pKa = 4.66KK67 pKa = 10.99DD68 pKa = 3.21LGTQTVVFNDD78 pKa = 3.62QIEE81 pKa = 4.35TGDD84 pKa = 3.79FFNSDD89 pKa = 3.41GLITGSATLCLYY101 pKa = 11.08SMNGSEE107 pKa = 5.18VPYY110 pKa = 10.94NDD112 pKa = 4.5LAVMMNNLNFAAPTVDD128 pKa = 5.26LGTTKK133 pKa = 10.47YY134 pKa = 8.25RR135 pKa = 11.84TSKK138 pKa = 10.95VIFKK142 pKa = 10.67SAVFDD147 pKa = 3.61LTLTNTSYY155 pKa = 11.67DD156 pKa = 3.61RR157 pKa = 11.84IGGVNNIEE165 pKa = 3.83AAYY168 pKa = 9.88SLEE171 pKa = 4.1VDD173 pKa = 2.92IHH175 pKa = 5.07TVVIKK180 pKa = 10.71KK181 pKa = 9.39GARR184 pKa = 11.84DD185 pKa = 3.35LDD187 pKa = 4.64FISGTLAEE195 pKa = 5.18KK196 pKa = 10.56GLKK199 pKa = 9.56TIDD202 pKa = 3.39KK203 pKa = 8.95MIEE206 pKa = 3.86VAEE209 pKa = 4.25KK210 pKa = 9.6EE211 pKa = 4.11VRR213 pKa = 11.84PIGGDD218 pKa = 3.16DD219 pKa = 3.42SKK221 pKa = 11.49IFNTTKK227 pKa = 10.72AKK229 pKa = 10.03FNRR232 pKa = 11.84GGTLWDD238 pKa = 3.69VPHH241 pKa = 6.87ALSLFGMKK249 pKa = 9.74ILSKK253 pKa = 10.42KK254 pKa = 10.36KK255 pKa = 9.55YY256 pKa = 8.96FIPGGATITYY266 pKa = 8.86QMRR269 pKa = 11.84DD270 pKa = 2.87AKK272 pKa = 10.36RR273 pKa = 11.84RR274 pKa = 11.84VMVPQFNMLGVNKK287 pKa = 10.08PGWTKK292 pKa = 10.4FLYY295 pKa = 8.68ITYY298 pKa = 10.17KK299 pKa = 10.39LVPGVGVIGQDD310 pKa = 3.29EE311 pKa = 4.27NVPTQIRR318 pKa = 11.84TRR320 pKa = 11.84LSVGVTRR327 pKa = 11.84KK328 pKa = 9.94YY329 pKa = 10.24SYY331 pKa = 10.61KK332 pKa = 10.05IEE334 pKa = 4.29GQNDD338 pKa = 2.67VRR340 pKa = 11.84DD341 pKa = 4.1NYY343 pKa = 11.34NPDD346 pKa = 3.03LL347 pKa = 4.52

Molecular weight: 39.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

661

314

347

330.5

38.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.782 ± 0.191

1.21 ± 0.699

5.9 ± 0.115

5.144 ± 1.278

4.085 ± 0.399

6.808 ± 1.394

1.362 ± 0.377

6.354 ± 0.229

8.926 ± 0.006

7.262 ± 0.48

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.35

5.446 ± 0.459

3.933 ± 0.36

2.874 ± 0.432

7.716 ± 1.142

5.598 ± 0.53

6.959 ± 1.498

6.657 ± 0.634

2.874 ± 1.524

4.69 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski